Mass Spectrometry (MS) Common to Proteomics Research:

Different Mass Spectrometry Types (i.e. MALDI vs. SELDI, Wiki Definitions)

MALDI-TOF-MS Profiling (Example MALDI Biotyper by Bruker Daltronics)

MALDI Vs. ESI (John Fenn Lecture)

MALDI-TOF/TOF-MS

ESI-LT-MS (Example of Linear Ion Trap by Thermo Finnigan)

FTICR-MS

Ion Mobility Mass Spectrometry (Overview of Synapt HDMS by Waters)

A Few MS Procedures Applications:

Tissue Imaging (hosted by Protein Discovery)

NCI videos explanation by Richard Caprioli, Ph.D. (Examples of Profiling/ Imaging MS)

NCI biomarker discovery (Various examples related to Biomarker Discovery)

Mass Spectrometry Video and Lecture Series from Bio-Alive

ITRAQ (quantitative MS)

SILAC (quantitative MS)

AQUA (quantitative MS)

Non-Tagged Comparative Proteomics

Video on Ultra thin MALDI MS

Common Ionization Processes in MS Driven Proteomics:

Collision Induced Dissociation (CID)

Electron Transfer Dissociation (ETD, Example from Thermo Finnigan)

Electron Capture Dissociation (ECD)

Infrared Multiple photon Dissociation (IRMPD)

Secondary Ion MS (SIMS)

Protein Extraction/ Separations/ Isolation Approaches:

Top-Down Vs. Bottom Up Overview

Video on 1D PAGE

2D PAGE Overview

Video on Protein PAGE Staining

Video on Western Blotting

Video on Laser Capture Micro-dissection (LCM)

Nano-HPLC definition

MuDPIT Overview

Shotgun Proteomics Definition

Ion Chromatography (IEC) Overview

General Chromatography Notes from GE