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Bringing together molecular biology, biochemistry and biophysics, groups skilled in PROTEOMICS & STRUCTURAL BIOLOGY provide insight into the macromolecular structures and interactions of proteins and nucleic acids to appreciate the impact of such on their function within the cell. Shared research facilities provide central access to consultation and cutting-edge technologies, including high-field NMR, Mass-spectrometry, nanoscale fabrication, x-ray crystallography, kinomics, and surface plasmon resonance.
Central Alabama High Field NMR Facility (UAB)
This Facility provides advice and consultation useful in structural biology and drug design and discovery efforts including: quality control in synthetic and medicinal chemistry drug discovery/design; determination of 3D structures of proteins and their complexes; protein complexes with low molecular weight compounds; STD-NMR screening; fragment-based discovery and design (lead optimization); membrane proteins in solution; and NMR of cells.
Cryo-Electron Microscopy Core (UAB)
This Core provides high resolution electron microscopy and tomography of stained and unstained specimens. It is equipped with an FEI Tecnai F20 cryo-electron microscope and a Leica UC6 cryo-microtome for ultrathin sectioning of frozen samples. The instruments are available for use after training or as a core service.
Kinomics Core (UAB)
Kinomics is the study of kinase signaling within cellular or tissue lysates. It can help elucidate cellular signaling pathways altered by treatment, or for comparison of different phenotypes. The PamGene PamStation Kinomic Array platform measures the phosphorylation of 144 tyrosine or 144 serine/threonine kinase substrates that are imprinted on PamChip microarrays. Changes in individual peptide phosphorylation are imaged with FITC conjugated phosphospecific antibodies, and signal is computer quantified in BioNavigator. Lists of altered peptides are then exported and analyzed for probable upstream kinases with tools such as Kinexus Phosphonet, as well as advanced Pathway Analysis and network modeling using GeneGo MetaCore.
Mass Spectrometry/ Proteomics (MSP) Shared Facility (UAB)
The goal of this shared facility is to provide state-of-the-art capabilities and training in mass spectrometry, proteomics, and bioanalytic technologies. It is organized into three modules: 1) Bioanalytical Separation and Sample Preparation, 2) Proteomics, and 3) Data Analysis and Bioinformatics that are supported by Master’s level and Ph.D. scientists who are experts in mass spectrometry, bioanalytical chemistry, statistics, systems biology, and information handling.
Multidisciplinary Molecular Interaction Core (UAB)
This Core supports a GE Biacore T200 optical biosensor which employs surface plasmon resonance (SPR) technology for monitoring label-free real-time biomolecular interactions between macromolecules such as proteins, nucleic acids, carbohydrates, and lipids, as well as small molecules. The instrumentation provides determinations of binding specificities, kinetics, affinity, concentration determination, and epitope mapping. The Core is available on a fee-per-use basis.
Nanoscale Materials Fabrication and Characterization Core (UAB)
This Core features state-of-the-art nanofabrication and characterization facilities, including facilities for the fabrication of nanostructured thin film coatings, nanoparticle synthesis, nanofibrous materials, and nanoscale patterning of surfaces using lithography. It also offers instruments for characterization of nanoscale materials using atomic force microscopy, x-ray photoelectron spectroscopy, thin-film x-ray diffraction, Raman and Infrared spectroscopy. The mechanical testing includes nanoindentation hardness, nanoscale tribological testing, and wear simulators for evaluation of biomedical implants.
X-Ray Crystallography Shared Facility (UAB)
This Facility includes capacity in x-ray differentiation data and analysis of coordinates for three dimensional structure determination of proteins and protein drug complexes. Capabilities include x-ray diffraction; crystallography computing (which provides a central computing network system for data processing, data transfer, structure determination, graphics, and database management in conjunction with all the latest software modeling programs available); and dedicated access to two of the most powerful beamlines at the Argonne National Laboratory in Chicago through the Southeast Regional Collaborative Access Team (SERCAT).
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The UAB CCTS is supported through the
National Institutes of Health (NIH) Clinical and Translational Science Award (CTSA) program
, grant UL1 TR000165.The CTSA program is led by the NIH’s
National Center for Advancing Translational Sciences (NCATS)
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