My research interest is to study the mechanisms of transcriptional and epigenetic regulations using hematopoiesis as a model system. Hematopoiesis is a tightly programmed epigenetic process through interplay between extracellular signaling and transcriptional regulation. Abnormal hematopoiesis i.e. leukemogenesis is caused by mutation or translocations of tyrosine kinases or transcription factors, which result in blockage of terminal differentiation and unchecked proliferation. As the hematopoietic stem cell (HSC) is differentiated into different lineage cells, chromatin structures of lineage specific genes are altered accordingly through the recruitment of chromatin remodeling proteins by transcription factors. Among different histone modifications, histone is arginine methylated. Arginine methyl transferases (PRMT1, PRMT4 and 5) are crucial in normal hematopoiesis and leukemogenesis. Not only histone but also transcription factor (RUNX1) critical for hematopoiesis is methylated by arginine methyl transferases (Gene and Development 2008). RUNX1, which is not methylated in hematopoietic stem cell, is methylated by PRMT1 and becomes a transcriptional activator when the HSC is differentiated into myeloid lineage.
My lab will continue to focus on arginine methyl transferases, in particular PRMT5, for their roles in hematopoiesis using cutting edge biochemical approaches as well as genetic approaches. We would like to address the following questions:
Dr. Xinyang Zhao graduated from Fudan University in Shanghai, China with a B.S. degree in Microbiology in 1990. He then gained M.S. and Ph.D. degrees in Microbiology from SUNY at Buffalo in 1996. After a shot postdoctoral training at University of Toronto, he moved to Howard Hughes Medical School in Cold Spring Harbor Lab to study transcriptional regulation in Dr. Nouria Hernandez’s lab. In 2003, Dr. Zhao joined the research group of Dr. Stephen Nimer at Memorial Sloan Kettering Cancer Center in New York where he was promoted to senior research scientist position. Dr. Zhao studied protein arginine methyltransferases for their roles in epigenetic and transcriptional regulation using leukemia as disease model. He joined the Department of Biochemistry and Molecular Genetics and Stem Cell Institute at UAB in August, 2011.