|Anupam Agarwal, M.D.
Professor of Medicine, Biochemistry and Molecular Genetics, and Cell Biology
Director of the Nephrology Research and Training Center
Areas of Focus: Molecular regulation of the human heme oxygenase-1 (HO-1) gene in renal injury and in atherosclerosis
Dr. Agarwal's research efforts include three main areas.
(i) His laboratory is studying the molecular regulation of the human heme oxygenase-1 (HO-1) gene in renal injury, transplant rejection, diabetic vascular disease and in atherosclerosis. Studies are also exploring the cellular mechanisms involved in the protective effects of the HO-1 enzyme in renal and vascular injury. Induction of this gene occurs as an adaptive and beneficial response to injury and is protective in several clinically important conditions such as acute renal failure, transplant rejection and atherosclerosis. Studies are focused on identifying regulatory regions in the human HO-1 gene that mediate induction in response to stimuli such as heme, cytokines, nitric oxide, modified lipids and growth factors (eg. transforming growth factor-beta). The studies involve molecular biology techniques to study DNA-protein interactions using chromatin structure analysis, in vivo footprinting, site-directed mutagenesis and gel shift assays. His laboratory is also studying the molecular and biological effects of nitrated lipids on HO-1 gene expression in endothelial cells and in the vasculature.
(ii) The functional significance of HO-1 gene expression is also being evaluated using both in vitro and in vivo systems in transgenic animal models of acute kidney injury, immune-mediated injury in the setting of transplantation, diabetes and atherosclerosis.
(iii) His laboratory is actively pursuing gene delivery approaches in the kidney and the vasculature in animal models of transplantation and diabetes using recombinant adeno-associated viral vectors. Alternate serotypes and capsid mutants of adeno-associated viral vectors are being developed to maximize gene transfer to the otherwise resistant vascular compartment with a goal towards targeting diabetic micro and macrovascular disease.
Dr. Agarwal graduated with honors from Kasturba Medical College in India. He completed his residency at the Postgraduate Institute of Medical Education and Research in India, where he also completed a nephrology fellowship.
Dr. Agarwal was a nephrology fellow at the University of Minnesota Hospital and Clinic in Minneapolis. He then joined the Division of Nephrology, Hypertension, and Transplantation at the University of Florida in Gainesville as an assistant professor and was promoted to tenured associate professor. He also served as the interim chief for research, prior to joining UAB. He was promoted to the rank of Professor at UAB in October 2005 and was named the Thomas E. Andreoli Professor of Nephrology in September 2006.