chip-selectorSNP Genotyping

The Heflin Center for Genomic Sciences provides SNP genotyping on five different platforms depending on the size and scope of your project. The Illumina BeadXpress system provides cost-effective genotyping for small multiplexes from 48-386 SNPs, and the Illumina iScan and the Affymetrix GeneChip systems can accommodate genotyping for more than 1 million SNPs in a single assay. For low-level genotyping, Pyrosequencing can be used for small numbers of SNPs on a limited number of samples, and the TaqMan Allelic Discrimination can be used for more moderate numbers of SNPs with larger population sizes. All of the above methods work on known SNPs and are not intended for SNP discovery. The options for genotyping using these platforms are:

Illumina Affymetrix Other
Cardio-MetaboChip DMET Plus Premier Pack
Pyrosequencing
ImmunoChip Genome-Wide Human SNP Array 5.0, MTO
TaqMan
GoldenGate Genome-Wide Human SNP Array 6.0
VeraCode
HumanCytoSNP
HumanExome
Human Linkage-24

HumanOmniExpress-12
HumanOmniExpressExome
HumanOmni5Exome

HumanOmni1-Quad

HumanOmni5-Quad

HumanOmni5-Quad

HumanOmni1S-8

HumanOmni2.5-8

HumanOmni2.5S-8

Cow
Dog
Pig
Sheep
Maize

The Core Facility maintains the Roche LightCycler 480 Real Time PCR machine for allelic discrimination.