Sanger Sequencing 

The Genomics Core Laboratory of the Heflin Center for Genomic Science offers fee based fluorescent DNA sequencing 3730DNA2services for both UAB and external clients. The Core Lab accepts CsCl purified plasmids, mini-prep plasmids, single stranded DNA, PCR products, and BACs; the Core Lab processes customer samples according to Applied Biosystems Big Dye Terminator v3.1 Ready Reaction kit protocol. In addition to standard sequencing analysis, Factura analysis is also available.

Turnaround time for sequencing is 2-3 business days. Samples are submitted and data retrieved through our secure server. Sequencing results are provided in .AB1 file format which can be viewed using either the Chromas (for PC) or 4Peaks (for MAC) programs.

The price list for our service:

Item UAB Non-UAB
Individual sequencing reaction $6/reaction

Contact Mike Crowley
Complete 96-well plate $408/plate
Quantitation/Dilution/Resuspension $2/reaction

For optimal results, please follow the guidelines in the Sample Preparation and Management section.