Analytic Imaging and Immunoreagent Core (AIIC) - Imaging Capabilities
- Last Updated on June 05, 2013
The AIIC-Imaging Component, estableshed in 1998, is a cutting-edge facility providing a variety of microscopy services for RDCC, CAMAC and UAB Scientists. Investigators conducting experiments that require the use of high end digital microscopes can benefit from the facility’s diverse applications. Fluorescence Microscopy, 2d and 3d Confocal Laser Microscopy, Time Lapse, FLIM, FRAP, FRET, Electron Microscopy, Cryo-EM Microscopy and Light Microscopy are just a few examples of techniques available in the core. Consultation and Training are available for all users of the facility by a staff of highly trained professionals.
If you are a
Assistance in Instrumentation or Sample Preparation
Basic Digital Imaging/Deconvolution/ Calcium Imaging -- Olympus IX70
Basic Digital Imaging –Leitz Orthoplan, Nikon Diaphot
Confocal Laser Scanning Microscopy – Leica SP1
Confocal Laser Scanning Microscopy with UV Laser – Leica
Confocal Laser Scanning Microscopy with UV Laser and FLIM / FRAP / FRET 3D capabilities - Zeiss LSM 710
Digital Imaging / Timelapse / FRET / Histology Imaging – Nikon Eclipse
FRET / FRAP Confocal Laser Scanning Microscopy – Leica SP2
2 Large Specimen/Tissue Dissection Imaging Nikon Stereoscope
Data Archival Service Fee (includes blank CD)
High Resolution Printing: Inkjet
Transmission Electron Microscopy--- FEI Tecnai T12 Spirit (Assisted and Unassisted Options)
Ultra-thin and semi-thin sectioning per sample with post stain
Negative Staining per Grid
Cryo-EM Usage Technai F20 (Full and Reduced Service Access Options Available)
Cryo-EM Grid for Sample Preparation
EDUCATION AND OUTREACH
While providing resources via specialized instrumentation and expertise are critical functions of a research core, of even greater importance are the intellectual scientific interactions that take place between core personnel and RDCC & CAMAC investigators wherein there is an active exchange of information relating to the biological processes under study. In addition to individual meetings, the AGTC offers more formal “brainstorming” sessions called Individualized Design of Experiments & Analyses Session (IDEAs). On a bi-weekly basis, the Director and Co-Directors will meet with individual AIIC users and their staff to review research projects in detail by examining primary data and experimental designs, and provide advice on future courses of action. Importantly, the IDEAs meetings provide investigators the opportunity to obtain feedback on a regular basis from 3 investigators highly experienced in imgaging and immunoreagent capabilities.
Additionally, the AIIC frequently sponsors on-campus seminars, lectures and workshops describing state-of-the-art imaging strategies and immunoreagent methodologies.
Professional Societies and Meetings
- Information on Fluorophores
- MPE Tutorial and Glossary
- Photonics Dictionary
- Cellular and Moleular Biology Dictionary
- Medical Dictionary
- On-line atlas of the rat brain
- Links to Key Researchers in the Imaging Field
- University of Wisconscin MPE Education Page
Please contact Dr. Keyser or Shawn Williams for current pricing.
Kent Keyser, PhD,
For questions pertaining to Light Microscopy, Fluorescent Microscopy, Confocal Microscopy, FLIM, FRET, FRAP, or general questions about the facility or microscopy please contact the following personnel
For questions pertaining to Electron Microscopy and EM Sample Preparation please contact the following personnel.