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Core FC

Core H redThe UAB Flow Cytometry Facility is comprised of two locations as a result of a consolidation between the flow cytometry cores of the Center for AIDS Research and the Comprehensive Arthritis, Musculoskeletal and Autoimmunity Center. These locations include BBRB (Bevill Biomedical Research Building, room 557) and Shelby (Shelby Biomedical Research Building, room 271). Our mission is to provide state-of-the-art cell sorting and analytical flow cytometry services to support research programs. The facility is made available to all Investigators at UAB and encourages collaborations and synergy.  Specific services and equipment at each location is listed below. Most services are provided at either location. Training, consultation, assisted- and self-serve equipment usage is available.

Locations

cfarcoreThe BBRB location provides the following equipment and services:

  • Cell sorting
    • - BD FACS Aria
  • Cell analysis
    • BD LSR II GUAVA EasyCyte
  • Bioplex analysis
  • High throughput flow cytometry
    • Hypercyt autosampler
apcfcoreThe Shelby location provides the following equipment and services:

  • Cell sorting
    • BD FACS Aria
  • Cell analysis
    • BD LSR II
    • FACS Calibur
    • FACS Scan
  • AutoMacs
    • MACS Sorter

 

Scheduling


First Time Users:
Please contact Theresa Newton at This email address is being protected from spambots. You need JavaScript enabled to view it.  to be set up as a user.

Once Ms. Newton confirms that you have been set up, you will receive an email invitation with a link to the Priority Software online scheduling site. This link will provide you with instructions on how to set your password and log in to the scheduler 

You now have Web Access to the BBRB Flow Cytometry Facility or the Shelby Flow Cytometry Facility. Your logon email address is:  This email address is being protected from spambots. You need JavaScript enabled to view it.

Questions:

If you have any questions or problems with the login, please contact Ms. Newton by This email address is being protected from spambots. You need JavaScript enabled to view it.  or phone: 205-975-7048.

Contact the facilities for questions related to capabilties, training, equipment or scheduling.

Flow Cytometry Web Scheduling

Services


In 2010, the CAMAC (Comprehensive Arthritis, Musculoskeletal and Autoimmunity Center) and the CFAR (Center for AIDS Research) Flow Cytometry Cores officially established a partnership through a federal core consolidation grant.  This funding was provided by an ARRA supplemental award (3P30AI027767-22S1, PI: Dr. Michael Saag) to the UAB CFAR. These efforts lead to the purchase of a new 4-laser - 14 parameter FACSAria sorter to the CAMAC Flow Core and an upgrade of the existing CFAR Flow Core FACSAria II sorter. As a result of this consolidation, expanded equipment and services are now provided to all researchers at UAB and beyond. Please see what is available to support your studies below or contact us for more information.


srv-hypercytautosampler
The HyperCytautosampler allows for high throughput flow cytometric analysis. Samples from 96- or 384 well plates are analyzed in a time resolved manner. The HyperCytautosampler is currently connected to a FACSCalibur, which allows for 3 color analysis.

HTS flow cytometry ideally suited for:
  • Large sample sets from patient studies
  • Hybridoma identification
  • Drug screening
Location: BBRB 557
 
srv-facsaria The FACSAria is a state of the art cell 4-laser sorter; 4 way sorting of up to 14 parameters is possible. The two machines have different filter and lasers set-ups.
Location: BBRB 557 and SHEL 271
srv-bioplex
The BioPlex suspension array system offers protein and nucleic acid researchers a reliable multiplex assay solution that permits analysis of up to 30biomolecules in a single sample.
BBRB 557
srv-bdlsr2 The LSR II is a state of the art 4-laser flow cytometer. Analysis can be up to 18 parameters; quantum-dot enabled.
Location: BBRB 557 and SHEL 271*
* An HTS system is available in SHEL 271
 
srv-facscalibur

FACSCalibur is a 2-laser flow cytometer that can detect up to four colors such as FITC, PE, PE-Cy5, or APC. A carousel system is available for automatic sample collection.
Location: SHEL 271
srv-facscan

FACScan is a user friendly flow cytometer that can detect up to three colors such, as FITC, PE, and PerCP or PE-Cy5.
Location: SHEL 271
srv-automacs The autoMACS™ Separator is a bench top instrument for high-speed automated cell sorting (Miltenyi) using magnetic beads. Positive and negative selection possible.
Location: SHEL 271


Fees


All Assisted Services Fees: $75 / hour

Self-Service Equipment Costs:
LSR II: $50 / hour
FACScan: $35 / hour
FACSCalibur: $35 / hour
BioPlex analysis: $50 / analyzed plate
HTS fees are negotiated on a per-project basis

Training Fees: Not all training has associated costs. Costs depend on the proficiency of the user and the type of equipment.

Contact the facility directly for more information or to schedule training for specific equipment.

Flow Cytometry Web Scheduling

Publications


Allogeneic Th1 Cells Home to Host Bone Marrow and Spleen and Mediate IFNγ-Dependent Aplasia. Chewning JH, Zhang W, Randolph DA, Swindle CS, Schoeb TR, Weaver CT. Biol Blood Marrow Transplant. 2013 Jun;19(6):876-887. PMID: 23523972

Retinoic acid hypersensitivity promotes peripheral tolerance in recent thymic emigrants. Bhaumik S, Giffon T, Bolinger D, Kirkman R, Lewis DB, Weaver CT, Randolph DA. J Immunol. 2013 Mar 15;190(6):2603-13. PMID: 23401586

Viral escape from neutralizing antibodies in early subtype A HIV-1 infection drives an increase in autologous neutralization breadth. Murphy MK, Yue L, Pan R, Boliar S, Sethi A, Tian J, Pfafferot K, Karita E, Allen SA, Cormier E, Goepfert PA, Borrow P, Robinson JE, Gnanakaran S, Hunter E, Kong XP, Derdeyn CA. PLoS Pathog. 2013 Feb;9(2):e1003173. PMID: 23468623

HIV-Specific CD8+ T Cells from Elite Controllers Are Primed for Survival. Yan J, Sabbaj S, Bansal A, Amatya N, Shacka JJ, Goepfert PA, Heath SL. J Virol. 2013 May;87(9):5170-81. PMID: 23449791

Hormonal contraception and HIV-1 infection: medroxyprogesterone acetate suppresses innate and adaptive immune mechanisms. Huijbregts RP, Helton ES, Michel KG, Sabbaj S, Richter HE, Goepfert PA, Hel Z. Endocrinology. 2013 Mar;154(3):1282-95. PMID: 23354099

Cutting Edge: Defective Follicular Exclusion of Apoptotic Antigens Due to Marginal Zone Macrophage Defects in Autoimmune BXD2 Mice. Li H, Wu Q, Li J, Yang P, Zhu Z, Luo B, Hsu HC, Mountz JD. J Immunol. 2013 May 1;190(9):4465-9. PMID: 23543760

Copper-boosting compounds: a novel concept for antimycobacterial drug discovery. Speer A, Shrestha TB, Bossmann SH, Basaraba RJ, Harber GJ, Michalek SM, Niederweis M, Kutsch O, Wolschendorf F. Antimicrob Agents Chemother. 2013 Feb;57(2):1089-91. PMID: 23254420

An AP-1 binding site in the enhancer/core element of the HIV-1 promoter controls the ability of HIV-1 to establish latent infection. Duverger A, Wolschendorf F, Zhang M, Wagner F, Hatcher B, Jones J, Cron RQ, van der Sluis RM, Jeeninga RE, Berkhout B, Kutsch O. J Virol. 2013 Feb;87(4):2264-77. PMID: 23236059

Estradiol Reduces Susceptibility of CD4(+) T Cells and Macrophages to HIV-Infection. Rodriguez-Garcia M, Biswas N, Patel MV, Barr FD, Crist SG, Ochsenbauer C, Fahey JV, Wira CR. PLoS One. 2013 Apr 17;8(4):e62069. PMID: 23614015

FCRL5 exerts binary and compartment-specific influence on innate-like B-cell receptor signaling. Zhu Z, Li R, Li H, Zhou T, Davis RS. Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):E1282-90. PMID: 23509253

AMP-activated protein kinase restricts IFN-γ signaling. Meares GP, Qin H, Liu Y, Holdbrooks AT, Benveniste EN. J Immunol. 2013 Jan 1;190(1):372-80. PMID: 23180823

The link between antibodies to OxLDL and natural protection against pneumococci depends on DH gene conservation. Vale AM, Kapoor P, Skibinski GA, Elgavish A, Mahmoud TI, Zemlin C, Zemlin M, Burrows PD, Nobrega A, Kearney JF, Briles DE, Schroeder HW Jr. J Exp Med. 2013 Apr 15. PMID: 23589567

Recirculating bone marrow B cells in C57BL/6 mice are more tolerant of highly hydrophobic and highly charged CDR-H3s than those in BALB/c mice. Khass M, Buckley K, Kapoor P, Schelonka RL, Watkins LS, Zhuang Y, Schroeder HW Jr. Eur J Immunol. 2013 Mar;43(3):629-40. PMID: 23225217

Lymphotoxin α1β2 expression on B cells is required for follicular dendritic cell activation during the germinal center response. Myers RC, King RG, Carter RH, Justement LB. Eur J Immunol. 2013 Feb;43(2):348-59. PMID: 23112125

Regulation of Th1 cells and experimental autoimmune encephalomyelitis by glycogen synthase kinase-3. Beurel E, Kaidanovich-Beilin O, Yeh WI, Song L, Palomo V, Michalek SM, Woodgett JR, Harrington LE, Eldar-Finkelman H, Martinez A, Jope RS. J Immunol. 2013 May 15;190(10):5000-11. PMID: 23606540

The actin and tetraspanin networks organize receptor nanoclusters to regulate B cell receptor-mediated signaling.Mattila PK, Feest C, Depoil D, Treanor B, Montaner B, Otipoby KL, Carter R, Justement LB, Bruckbauer A, Batista FD. Immunity. 2013 Mar 21;38(3):461-74. PMID: 23499492

Lymphotoxin α1β2 expression on B cells is required for follicular dendritic cell activation during the germinal center response. Myers RC, King RG, Carter RH, Justement LB. Eur J Immunol. 2013 Feb;43(2):348-59. PMID: 23112125

Helicobacter pylori Infection Inhibits Phagocyte Clearance of Apoptotic Gastric Epithelial Cells. Bimczok D, Smythies LE, Waites KB, Grams JM, Stahl RD, Mannon PJ, Peter S, Wilcox CM, Harris PR, Das S, Ernst PB, Smith PD. J Immunol. 2013 May 17. PMID: 23686492

Downregulated Th17 responses are associated with reduced gastritis in Helicobacter pylori-infected children. Serrano C, Wright SW, Bimczok D, Shaffer CL, Cover TL, Venegas A, Salazar MG, Smythies LE, Harris PR, Smith PD. Mucosal Immunol. 2013 Jan 9. PMID: 23299619

PECAM1(+)/Sca1(+)/CD38(+) vascular cells transform into myofibroblast-like cells in skin wound repair. Etich J, Bergmeier V, Frie C, Kreft S, Bengestrate L, Eming S, Mauch C, Eckes B, Ulus H, Lund FE, Rappl G, Abken H, Paulsson M, Brachvogel B. PLoS One. 2013;8(1):e53262. PMID: 23308177

Interleukin-17-dependent CXCL13 mediates mucosal vaccine-induced immunity against tuberculosis. Gopal R, Rangel-Moreno J, Slight S, Lin Y, Nawar HF, Fallert Junecko BA, Reinhart TA, Kolls J, Randall TD, Connell TD, Khader SA. Mucosal Immunol. 2013 Jan 9.  PMID: 23299616

CXCR5? T helper cells mediate protective immunity against tuberculosis. Slight SR, Rangel-Moreno J, Gopal R, Lin Y, Fallert Junecko BA, Mehra S, Selman M, Becerril-Villanueva E, Baquera-Heredia J, Pavon L, Kaushal D, Reinhart TA, Randall TD, Khader SA. J Clin Invest. 2013 Feb 1;123(2):712-26. PMID: 23281399

Useful Links


A 12 minute video introduction to flow cytometry: A must see for beginners :
http://probes.invitrogen.com/resources/education/tutorials/4Intro_Flow/player.html 

Flow Cytometry – A Basic Introduction by Michael G. Ormerod
http://flowbook.denovosoftware.com/ 

Practical Flow Cytometry --- Howard Shapiro
http://www.beckmancoulter.com/wsrportal/wsr/research-and-discovery/products-and-services/flow-cytometry/practical-flow-cytometry/index.htm 

A useful Java applet put together by Joe Trotter of BD Biosciences. You can view the excitation and emission spectra of many common fluorescent dyes for help in panel design.
http://www.bdbiosciences.com/research/multicolor/tools/index.jsp 

An applet similar to the above that has all of the Invtrogen reagents, as well as common fluorchromes
http://www.invitrogen.com/site/us/en/home/support/Research-Tools/Fluorescence-SpectraViewer.html

For FlowJo users: http://flowjo.typepad.com/ 

Excellent information on flow cytometry applications, especially intracellular staining
http://maeckerlab.typepad.com/maeckerlab_weblog/maeckerlab_protocols_and_tools/ 

Invitrogen: The current home of the legendary Molecular Probes materials and lots of other useful information:
http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-and-Tissue-Analysis/Flow-Cytometry.html 

Cell Signaling
http://www.cellsignal.com/technologies/flow.html 

Millipore GUAVA
http://www.millipore.com/flowcytometry/fc/fchome&open&cid=U0021 

Intellicyt --- HyperCyt autosampler for high throughput flow cytometry (available in CFAR Core)
http://www.intellicyt.com/instruments/hypercyt-sampler

Contact Information


BBRB Location:

Director: Olaf Kutsch, PhD
Manager: Marion Spell, BS
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
Phone: (205) 975-3227

Bevill Basic Research Building
Room 557
845 19th Street South
Birmingham, Alabama, 35294

Shelby Location

Director: John D. Mountz, MD, PhD
Manager: Enid F. Keyser, BS
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.
(205) 934-1362

Shelby Biomedical Research Building
Room 271
1825 University Blvd
Birmingham, Alabama, 35294