DURING THE UPCOMING BUDGET PERIOD, THE FLOW CYTOMETRY CORE WILL TRANSITION TO THE NEW BASIC AND TRANSLATIONAL SCIENCES CORE. SERVICES OF THE FORMER VIROLOGY AND GENOMICS CORES WILL BE INCLUDED. CHECK BACK FOR INFORMATION REGARDING THESE CHANGES.
The UAB Flow Cytometry Facility is comprised of two locations as a result of a consolidation between the flow cytometry cores of the Center for AIDS Research and the Comprehensive Arthritis, Musculoskeletal and Autoimmunity Center. These locations include BBRB (Bevill Biomedical Research Building, room 557) and Shelby (Shelby Biomedical Research Building, room 271). Our mission is to provide state-of-the-art cell sorting and analytical flow cytometry services to support research programs. The facility is made available to all Investigators at UAB and encourages collaborations and synergy. Specific services and equipment at each location is listed below. Most services are provided at either location. Training, consultation, assisted- and self-serve equipment usage is available.
|The BBRB location provides the following equipment and services:
||The Shelby location provides the following equipment and services:
First Time Users:
Please contact Jena L. Reeves at email@example.com to be set up as a user.
Once Ms. Reeves confirms that you have been set up, you will receive an email invitation with a link to the Priority Software online scheduling site. This link will provide you with instructions on how to set your password and log in to the scheduler.
You now have Web Access to the BBRB Flow Cytometry Facility or the Shelby Flow Cytometry Facility. Your logon email address is: firstname.lastname@example.org
If you have any questions or problems with the login, please contact Ms. Reeves by email or phone: 205-934-7753.
Contact the facilities for questions related to capabilties, training, equipment or scheduling.
In 2010, the CAMAC (Comprehensive Arthritis, Musculoskeletal and Autoimmunity Center) and the CFAR (Center for AIDS Research) Flow Cytometry Cores officially established a partnership through a federal core consolidation grant. This funding was provided by an ARRA supplemental award (3P30AI027767-22S1, PI: Dr. Michael Saag) to the UAB CFAR. These efforts lead to the purchase of a new 4-laser - 14 parameter FACSAria sorter to the CAMAC Flow Core and an upgrade of the existing CFAR Flow Core FACSAria II sorter. As a result of this consolidation, expanded equipment and services are now provided to all researchers at UAB and beyond. Please see what is available to support your studies below or contact us for more information.
|The BD Accuri C6 Cytometer system is equipped with a blue and red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of FITC, PE, PerCP, and APC. A compact optical design, fixed alignment, and pre-optimized detector settings make the system easier to use.
Location: BBRB 557
|The HyperCytautosampler allows for high throughput flow cytometric analysis. Samples from 96- or 384 well plates are analyzed in a time resolved manner. The HyperCytautosampler is currently connected to a FACSCalibur, which allows for 3 color analysis.
HTS flow cytometry ideally suited for:
|The FACSAria is a state of the art cell 4-laser sorter; 4 way sorting of up to 14 parameters is possible. The two machines have different filter and lasers set-ups.
Location: BBRB 557 and SHEL 271
The BioPlex suspension array system offers protein and nucleic acid researchers a reliable multiplex assay solution that permits analysis of up to 30biomolecules in a single sample.
|The LSR II is a state of the art 4-laser flow cytometer. Analysis can be up to 18 parameters; quantum-dot enabled.
Location: BBRB 557 and SHEL 271*
* An HTS system is available in SHEL 271
|FACSCalibur is a 2-laser flow cytometer that can detect up to four colors such as FITC, PE, PE-Cy5, or APC. A carousel system is available for automatic sample collection.
Location: SHEL 271
|FACScan is a user friendly flow cytometer that can detect up to three colors such, as FITC, PE, and PerCP or PE-Cy5.
Location: SHEL 271
|The autoMACS™ Separator is a bench top instrument for high-speed automated cell sorting (Miltenyi) using magnetic beads. Positive and negative selection possible.
Location: SHEL 271
All Assisted Services Fees: $75 / hour
Self-Service Equipment Costs:
LSR II: $50 / hour
FACScan: $35 / hour
FACSCalibur: $35 / hour
BioPlex analysis: $50 / analyzed plate
HTS fees are negotiated on a per-project basis
Training Fees: Not all training has associated costs. Costs depend on the proficiency of the user and the type of equipment.
Contact the facility directly for more information or to schedule training for specific equipment.
Flow Cytometry Web Scheduling
1.) Moylan DC, Goepfert PA, Kempf MC, Saag MS, Richter HE, Mestecky J, Sabbaj S. Diminished CD103 (αEβ7) Expression on Resident T Cells from the Female Genital Tract of HIV-Positive Women. Pathog Immun. 2016 Fall-Winter;1(2):371-387. doi: 10.20411/pai.v1i2.166.
2.) Hel Z, Xu J, Denning WL, Helton ES, Huijbregts RP, Heath SL, Overton ET, Christmann BS, Elson CO, Goepfert PA, Mestecky J. Dysregulation of Systemic and Mucosal Humoral Responses to Microbial and Food Antigens as a Factor Contributing to Microbial Translocation and Chronic Inflammation in HIV-1 Infection. PLoS Pathog. 2017 Jan 26;13(1):e1006087. doi: 10.1371/journal.ppat.1006087.
3.) Prentice HA, Lu H, Price MA, Kamali A, Karita E, Lakhi S, Sanders EJ, Anzala O, Allen S, Goepfert PA, Hunter E, Gilmour J, Tang J. Dynamics and Correlates of CD8 T-Cell Counts in Africans with Primary Human Immunodeficiency Virus Type 1 Infection J Virol. 2016 Oct 28;90(22):10423-10430.
4.) Mónaco DC, Dilernia DA, Fiore-Gartland A, Yu T, Prince JL, Dennis KK, Qin K, Schaefer M, Claiborne DT, Kilembe W, Tang J, Price MA, Farmer P, Gilmour J, Bansal A, Allen S, Goepfert P, Hunter E. Balance between transmitted HLA preadapted and nonassociated polymorphisms is a major determinant of HIV-1 disease progression. J Exp Med. 2016 Sep 19;213(10):2049-63. doi: 10.1084/jem.20151984.
5.) Carlson JM, Du VY, Pfeifer N, Bansal A, Tan VY, Power K, Brumme CJ, Kreimer A, DeZiel CE, Fusi N, Schaefer M, Brockman MA, Gilmour J, Price MA, Kilembe W, Haubrich R, John M, Mallal S, Shapiro R, Frater J, Harrigan PR, Ndung'u T, Allen S, Heckerman D, Sidney J, Allen TM, Goulder PJ, Brumme ZL, Hunter E, Goepfert PA. Impact of pre-adapted HIV transmission. Nat Med. 2016 Jun;22(6):606-13. doi: 10.1038/nm.4100.
6.) Jordan SJ, Gupta K, Ogendi BM, Bakshi RK, Kapil R, Press CG, Sabbaj S, Lee JY, Geisler WM. The Predominant CD4+ Th1 Cytokine Elicited to Chlamydia trachomatis Infection in Women is Tumor Necrosis Factor-Alpha, not Interferon-Gamma. Clin Vaccine Immunol. 2017 Jan 18. pii: CVI.00010-17. doi: 10.1128/CVI.00010-17. [Epub ahead of print]
PMCID: Not assigned
7.) Matthews QL, Farrow AL, Rachakonda G, Gu L, Nde P, Krendelchtchikov A, Pratap S, Sakhare SS, Sabbaj S, Lima MF, Villalta F. Epitope Capsid-Incorporation: New Effective Approach for Vaccine Development for Chagas Disease. Pathog Immun. 2016 Fall-Winter;1(2):214-233.
8.) Schaaf K, Smith SR, Duverger A, Wagner F, Wolschendorf F, Westfall AO, Kutsch O, Sun J. Mycobacterium tuberculosis exploits the PPM1A signaling pathway to block host macrophage apoptosis. Sci Rep. 2017 Feb 8;7:42101. doi: 10.1038/srep42101.
9.) Schaaf K, Hayley V, Speer A, Wolschendorf F, Niederweis M, Kutsch O, Sun J. A Macrophage Infection Model to Predict Drug Efficacy Against Mycobacterium Tuberculosis. Assay Drug Dev Technol. 2016 Aug;14(6):345-54. doi: 10.1089/adt.2016.717.
10.) Yoder AC, Guo K, Dillon SM, Phang T, Lee EJ, Harper MS, Helm K, Kappes JC, Ochsenbauer C, McCarter MD, Wilson CC, Santiago ML. The transcriptome of HIV-1 infected intestinal CD4+ T cells exposed to enteric bacteria. PLoS Pathog. 2017 Feb 27;13(2):e1006226. doi: 10.1371/journal.ppat.1006226. [Epub ahead of print]
PMCID: Not assigned
11.) Rodriguez-Garcia M, Shen Z, Barr FD, Boesch AW, Ackerman ME, Kappes JC, Ochsenbauer C, Wira CR. Dendritic cells from the human female reproductive tract rapidly capture and respond to HIV. Mucosal Immunol. 2016 Aug 31. doi: 10.1038/mi.2016.72. [Epub ahead of print]
12.) Yang R, Masters AR, Fortner KA, Champagne DP, Yanguas-Casás N, Silberger DJ, Weaver CT, Haynes L, Rincon M. J IL-6 promotes the differentiation of a subset of naive CD8+ T cells into IL-21-producing B helper CD8+ T cells. Exp Med. 2016 Oct 17;213(11):2281-2291.
13.) Clement M, Marsden M, Stacey MA, Abdul-Karim J, Gimeno Brias S, Costa Bento D, Scurr MJ, Ghazal P, Weaver CT, Carlesso G, Clare S, Jones SA, Godkin A, Jones GW, Humphreys IR. Cytomegalovirus-Specific IL-10-Producing CD4+ T Cells Are Governed by Type-I IFN-Induced IL-27 and Promote Virus Persistence. PLoS Pathog. 2016 Dec 7;12(12):e1006050. doi: 10.1371/journal.ppat.1006050.
14.) Dalecki AG, Malalasekera AP, Schaaf K, Kutsch O, Bossmann SH, Wolschendorf F. Combinatorial phenotypic screen uncovers unrecognized family of extended thiourea inhibitors with copper-dependent anti-staphylococcal activity. Metallomics. 2016 Apr;8(4):412-21. doi: 10.1039/c6mt00003g.
15.) Sun J, Schaaf K, Duverger A, Wolschendorf F, Speer A, Wagner F, Niederweis M, Kutsch O. Protein phosphatase, Mg2+/Mn2+-dependent 1A controls the innate antiviral and antibacterial response of macrophages during HIV-1 and Mycobacterium tuberculosis infection. Oncotarget. 2016 Mar 29;7(13):15394-409. doi: 10.18632/oncotarget.8190.
16.) Shah S, Dalecki AG, Malalasekera AP, Crawford CL, Michalek SM, Kutsch O, Sun J, Bossmann SH, Wolschendorf F. 8-Hydroxyquinolines Are Boosting Agents of Copper-Related Toxicity in Mycobacterium tuberculosis. Antimicrob Agents Chemother. 2016 Sep 23;60(10):5765-76. doi: 10.1128/AAC.00325-16.
17.) Munguía-Fuentes R, Yam-Puc JC, Silva-Sánchez A, Marcial-Juárez E, Gallegos-Hernández IA, Calderón-Amador J, Randall TD, Flores-Romo L. Immunization of Newborn Mice Accelerates the Architectural Maturation of Lymph Nodes, But AID-Dependent IgG Responses Are Still Delayed Compared to the Adult. Front Immunol. 2017 Jan 19;8:13. doi: 10.3389/fimmu.2017.00013.
18.) Silva-Sanchez A, Randall TD. Fugue G Minor: Getting the Lymph Node Ensemble Together with Circadian Rhythm. Immunity. 2017 Jan 17;46(1):6-8. doi: 10.1016/j.immuni.2017.01.001.
PMCID: Not assigned
19.) Ballesteros-Tato A, Randall TD, Lund FE, Spolski R, Leonard WJ, León B. T Follicular Helper Cell Plasticity Shapes Pathogenic T Helper 2 Cell-Mediated Immunity to Inhaled House Dust Mite. Immunity. 2016 Feb 16;44(2):259-73. doi: 10.1016/j.immuni.2015.11.017.
A 12 minute video introduction to flow cytometry: A must see for beginners :
Flow Cytometry – A Basic Introduction by Michael G. Ormerod
Practical Flow Cytometry --- Howard Shapiro
A useful Java applet put together by Joe Trotter of BD Biosciences. You can view the excitation and emission spectra of many common fluorescent dyes for help in panel design.
An applet similar to the above that has all of the Invtrogen reagents, as well as common fluorchromes
For FlowJo users: http://flowjo.typepad.com/
Excellent information on flow cytometry applications, especially intracellular staining
Invitrogen: The current home of the legendary Molecular Probes materials and lots of other useful information:
Intellicyt --- HyperCyt autosampler for high throughput flow cytometry (available in CFAR Core)
BBRB Location:Director: Olaf Kutsch, PhD
Manager: Marion Spell, BS
Phone: (205) 975-3227
Bevill Basic Research Building
845 19th Street South
Birmingham, Alabama, 35294
Shelby LocationDirector: John D. Mountz, MD, PhD
Manager: Enid F. Keyser, BS
Shelby Biomedical Research Building
1825 University Blvd
Birmingham, Alabama, 35294