Core FC

Core H redThe UAB Flow Cytometry Facility is comprised of two locations as a result of a consolidation between the flow cytometry cores of the Center for AIDS Research and the Comprehensive Arthritis, Musculoskeletal and Autoimmunity Center. These locations include BBRB (Bevill Biomedical Research Building, room 557) and Shelby (Shelby Biomedical Research Building, room 271). Our mission is to provide state-of-the-art cell sorting and analytical flow cytometry services to support research programs. The facility is made available to all Investigators at UAB and encourages collaborations and synergy.  Specific services and equipment at each location is listed below. Most services are provided at either location. Training, consultation, assisted- and self-serve equipment usage is available.


cfarcoreThe BBRB location provides the following equipment and services:

  • Newest Instrumentation
    • BD Accuri C6 Flow Cytometer
  • Cell sorting
    • BD FACS Aria
  • Cell analysis
    • BD LSR II GUAVA EasyCyte
  • Bioplex analysis
  • High throughput flow cytometry
    • Hypercyt autosampler
apcfcoreThe Shelby location provides the following equipment and services:

  • Cell sorting
    • BD FACS Aria
  • Cell analysis
    • BD LSR II
    • FACS Calibur
    • FACS Scan
  • AutoMacs
    • MACS Sorter



First Time Users:
Please contact Theresa Newton at thenson@uab.edu to be set up as a user.

Once Ms. Newton confirms that you have been set up, you will receive an email invitation with a link to the Priority Software online scheduling site. This link will provide you with instructions on how to set your password and log in to the scheduler 

You now have Web Access to the BBRB Flow Cytometry Facility or the Shelby Flow Cytometry Facility. Your logon email address is:  blazerid@uab.edu


If you have any questions or problems with the login, please contact Ms. Newton by email or phone: 205-975-7048.

Contact the facilities for questions related to capabilties, training, equipment or scheduling.

Flow Cytometry Web Scheduling


In 2010, the CAMAC (Comprehensive Arthritis, Musculoskeletal and Autoimmunity Center) and the CFAR (Center for AIDS Research) Flow Cytometry Cores officially established a partnership through a federal core consolidation grant.  This funding was provided by an ARRA supplemental award (3P30AI027767-22S1, PI: Dr. Michael Saag) to the UAB CFAR. These efforts lead to the purchase of a new 4-laser - 14 parameter FACSAria sorter to the CAMAC Flow Core and an upgrade of the existing CFAR Flow Core FACSAria II sorter. As a result of this consolidation, expanded equipment and services are now provided to all researchers at UAB and beyond. Please see what is available to support your studies below or contact us for more information.

accuri overview The BD Accuri C6 Cytometer system is equipped with a blue and red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of FITC, PE, PerCP, and APC. A compact optical design, fixed alignment, and pre-optimized detector settings make the system easier to use. 

Location: BBRB 557

The HyperCytautosampler allows for high throughput flow cytometric analysis. Samples from 96- or 384 well plates are analyzed in a time resolved manner. The HyperCytautosampler is currently connected to a FACSCalibur, which allows for 3 color analysis.

HTS flow cytometry ideally suited for:
  • Large sample sets from patient studies
  • Hybridoma identification
  • Drug screening
Location: BBRB 557
srv-facsaria The FACSAria is a state of the art cell 4-laser sorter; 4 way sorting of up to 14 parameters is possible. The two machines have different filter and lasers set-ups.
Location: BBRB 557 and SHEL 271
The BioPlex suspension array system offers protein and nucleic acid researchers a reliable multiplex assay solution that permits analysis of up to 30biomolecules in a single sample.
BBRB 557
srv-bdlsr2 The LSR II is a state of the art 4-laser flow cytometer. Analysis can be up to 18 parameters; quantum-dot enabled.
Location: BBRB 557 and SHEL 271*
* An HTS system is available in SHEL 271

FACSCalibur is a 2-laser flow cytometer that can detect up to four colors such as FITC, PE, PE-Cy5, or APC. A carousel system is available for automatic sample collection.
Location: SHEL 271

FACScan is a user friendly flow cytometer that can detect up to three colors such, as FITC, PE, and PerCP or PE-Cy5.
Location: SHEL 271
srv-automacs The autoMACS™ Separator is a bench top instrument for high-speed automated cell sorting (Miltenyi) using magnetic beads. Positive and negative selection possible.
Location: SHEL 271


All Assisted Services Fees: $75 / hour

Self-Service Equipment Costs:
LSR II: $50 / hour
FACScan: $35 / hour
FACSCalibur: $35 / hour
BioPlex analysis: $50 / analyzed plate
HTS fees are negotiated on a per-project basis

Training Fees: Not all training has associated costs. Costs depend on the proficiency of the user and the type of equipment.

Contact the facility directly for more information or to schedule training for specific equipment.

Flow Cytometry Web Scheduling


Ballesteros-Tato A, Stone SL, Lund FE. Innate IFNγ-producing B cells.
Cell Res. 2014 Feb;24(2):135-6
PMID number: 24343577
PMCID number: PMC3915907

Ballesteros-Tato A, Randall TD. Priming of T follicular helper cells by dendritic cells.
Immunol Cell Biol. 2014 Jan;92(1):22-7
PMID number: 24145854
PMCID number: PMC4052723

Ballesteros-Tato A, León B, Lee BO, Lund FE, Randall TD. Epitope-specific regulation of memory programming by differential duration of antigen presentation to influenza-specific CD8(+) T cells.
Immunity. 2014 Jul 17;41(1):127-40
PMID number: 25035957
PMCID number: PMC4233138

Basu R, Whitley SK, Bhaumik S, Zindl CL, Schoeb TR, Benveniste EN, Pear WS, Hatton RD, Weaver CT.
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PMID number: 25642823
PMCID number: in process

Baxter AE, Russell RA, Duncan CJ, Moore MD, Willberg CB, Pablos JL, Finzi A, Kaufmann DE, Ochsenbauer C, Kappes JC, Groot F, Sattentau QJ. Macrophage infection via selective capture of HIV-1-infected CD4+ T cells. Cell Host Microbe. 2014 Dec 10;16(6):711-21
PMID number: 25467409
PMCID number: PMC4271767

Bet A, Sterrett S, Sato A, Bansal A, Goepfert PA. Characterization of T-cell responses to cryptic epitopes in recipients of a noncodon-optimized HIV-1 vaccine.
J Acquir Immune Defic Syndr. 2014 Feb 1;65(2):142-50
PMID number: 24442221
PMCID number: PMC3896890

Bharaj P, Chahar HS, Alozie OK, Rodarte L, Bansal A, Goepfert PA, Dwivedi A, Manjunath N, Shankar P. Characterization of programmed death-1 homologue-1 (PD-1H) expression and function in normal and HIV infected individuals.
PLoS One. 2014 Oct 3;9(10):e109103
PMID number: 25279955
PMCID number: PMC4184823

Bimczok D, Kao JY, Zhang M, Cochrun S, Mannon P, Peter S, Wilcox CM, Mönkemüller KE, Harris PR, Grams JM, Stahl RD, Smith PD, Smythies LE. Human gastric epithelial cells contribute to gastric immune regulation by providing retinoic acid to dendritic cells.
Mucosal Immunol. 2014 Sep 24
PMID number: 25249167
PMCID number: in process

Bowers NL, Helton ES, Huijbregts RP, Goepfert PA, Heath SL, Hel Z. Immune suppression by neutrophils in HIV-1 infection: role of PD-L1/PD-1 pathway.
PLoS Pathog. 2014 Mar 13;10(3)
PMID number: 24626392
PMCID number: PMC3953441

Cao AT, Yao S, Stefka AT, Liu Z, Qin H, Liu H, Evans-Marin HL, Elson CO, Nagler CR, Cong Y. TLR4 regulates IFN-γ and IL-17 production by both thymic and induced Foxp3+ Tregs during intestinal inflammation. J Leukoc Biol. 2014 Nov;96(5):895-905.
PMID number: 25015957
PMCID number: PMC4197555

Carlson JM, Schaefer M, Monaco DC, Batorsky R, Claiborne DT, Prince J, Deymier MJ, Ende ZS, Klatt NR, DeZiel CE, Lin TH, Peng J, Seese AM, Shapiro R, Frater J, Ndung'u T, Tang J, Goepfert P, Gilmour J, Price MA, Kilembe W, Heckerman D, Goulder PJ, Allen TM, Allen S, Hunter E. HIV transmission. Selection bias at the heterosexual HIV-1 transmission bottleneck.
Science. 2014 Jul 11;345(6193)
PMID number: 25013080
PMCID number: PMC4289910

Chewning JH, Weaver CT. Development and survival of Th17 cells within the intestines: the influence of microbiome- and diet-derived signals. J Immunol. 2014 Nov 15;193(10):4769-77.
PMID number: 25381358
PMCID number: in process

Ding Y, Li J, Yang P, Luo B, Wu Q, Zajac AJ, Wildner O, Hsu HC, Mountz JD. Interleukin-21 promotes germinal center reaction by skewing the follicular regulatory T cell to follicular helper T cell balance in autoimmune BXD2 mice.
Arthritis Rheumatol. 2014 Sep;66(9):2601-12
PMID number: 24909430
PMCID number: PMC4146687

Duverger A, Wolschendorf F, Anderson JC, Wagner F, Bosque A, Shishido T, Jones J, Planelles V, Willey C, Cron RQ, Kutsch O. Kinase control of latent HIV-1 infection: PIM-1 kinase as a major contributor to HIV-1 reactivation.
J Virol. 2014 Jan;88(1):364-76
PMID number: 24155393
PMCID number: PMC3911731

Guedes AG, Jude JA, Paulin J, Rivero-Nava L, Kita H, Lund FE, Kannan MS. Airway responsiveness in CD38-deficient mice in allergic airway disease: studies with bone marrow chimeras.
Am J Physiol Lung Cell Mol Physiol. 2015 Jan 9
PMID number: 25575514
PMCID number: in process

Haeili M, Moore C, Davis CJ, Cochran JB, Shah S, Shrestha TB, Zhang Y, Bossmann SH, Benjamin WH, Kutsch O, Wolschendorf F. Copper complexation screen reveals compounds with potent antibiotic properties against methicillin-resistant Staphylococcus aureus.
Antimicrob Agents Chemother. 2014 Jul;58(7):3727-36
PMID number: 24752262
PMCID number: PMC4068557

Huijbregts RP, Michel KG, Hel Z. Effect of progestins on immunity: medroxyprogesterone but not norethisterone or levonorgestrel suppresses the function of T cells and pDCs.
Contraception. 2014 Aug;90(2):123-9
PMID number: 24674041
PMCID number: in process

Kahan SM, Zajac AJ. A stay of execution: type I interferons pardon T cells from death by natural killers.
Immunity. 2014 Jun 19;40(6):861-2
PMID number: 24950208
PMCID number: in process

León B, Ballesteros-Tato A, Randall TD, Lund FE. Prolonged antigen presentation by immune complex-binding dendritic cells programs the proliferative capacity of memory CD8 T cells. J Exp Med. 2014 Jul 28;211(8):1637-55
PMID number: 25002751
PMCID number: PMC4113940

León B, Bradley JE, Lund FE, Randall TD, Ballesteros-Tato A. FoxP3+ regulatory T cells promote influenza-specific Tfh responses by controlling IL-2 availability. Nat Commun. 2014 Mar 17;5:3495
PMID number: 24633065
PMCID number: PMC4013682

León B, Ballesteros-Tato A, Lund FE. Dendritic cells and B cells: unexpected partners in Th2 development.
J Immunol. 2014 Aug 15;193(4):1531-7
PMID number: 25086176
PMCID number: PMC4233146

Li H, Goepfert P, Reeves RK. Short communication: Plasmacytoid dendritic cells from HIV-1 Elite Controllers maintain a gut-homing phenotype associated with immune activation.
AIDS Res Hum Retroviruses. 2014 Dec;30(12):1213-5
PMID number: 25387330
PMCID number: in process

Li H, Hsu HC, Wu Q, Yang P, Li J, Luo B, Oukka M, Steele CH 3rd, Cua DJ, Grizzle WE, Mountz JD. IL-23 promotes TCR-mediated negative selection of thymocytes through the upregulation of IL-23 receptor and RORγt. Nat Commun. 2014 Jul 8;5:4259
PMID number: 25001511
PMCID number: PMC4136447

Li J, Hsu HC, Ding Y, Li H, Wu Q, Yang P, Luo B, Rowse AL, Spalding DM, Bridges SL Jr, Mountz JD.
Inhibition of fucosylation reshapes inflammatory macrophages and suppresses type II collagen-induced arthritis. Arthritis Rheumatol. 2014 Sep;66(9):2368-79.
PMID number:
PMCID number:

Liu HP, Cao AT, Feng T, Li Q, Zhang W, Yao S, Dann SM, Elson CO, Cong Y. TGF-β converts Th1 cells into Th17 cells through stimulation of Runx1 expression. Eur J Immunol. 2015 Jan 20.
PMID number: 25605286
PMCID number: in process

Overton ET, Sterrett S, Westfall AO, Kahan SM, Burkholder G, Zajac AJ, Goepfert PA, Bansal A.

AIDS. 2014 Nov 13;28(17):2627-31 Effects of atorvastatin and pravastatin on immune activation and T-cell function in antiretroviral therapy-suppressed HIV-1-infected patients.

PMID number: 25569444

PMCID number: in process

Raska M, Czernekova L, Moldoveanu Z, Zachova K, Elliott MC, Novak Z, Hall S, Hoelscher M, Maboko L, Brown R, Smith PD, Mestecky J, Novak J. Differential glycosylation of envelope gp120 is associated with differential recognition of HIV-1 by virus-specific antibodies and cell infection. AIDS Res Ther. 2014 Aug 1;11:23

PMID number: 25120578

PMCID number: PMC4130436

Shen R, Kappes JC, Smythies LE, Richter HE, Novak L, Smith PD. Vaginal myeloid dendritic cells transmit founder HIV-1. J Virol. 2014 Jul;88(13):7683-8

PMID number: 24741097

PMCID number: PMC4054437

Shen R, Raska M, Bimczok D, Novak J, Smith PD. HIV-1 envelope glycan moieties modulate HIV-1 transmission. J Virol. 2014 Dec;88(24):14258-67

PMID number: 25275130

PMCID number: PMC4249159

Shen R, Richter HE, Smith PD. Interactions between HIV-1 and mucosal cells in the female reproductive tract.

Am J Reprod Immunol. 2014 Jun;71(6):608-17

PMID number: 24689653

PMCID number: PMC4073589

Wang H, Geng J, Wen X, Bi E, Kossenkov AV, Wolf AI, Tas J, Choi YS, Takata H, Day TJ, Chang LY, Sprout SL, Becker EK, Willen J, Tian L, Wang X, Xiao C, Jiang P, Crotty S, Victora GD, Showe LC, Tucker HO, Erikson J, Hu H. The transcription factor Foxp1 is a critical negative regulator of the differentiation of follicular helper T cells. Nat Immunol. 2014 Jul;15(7):667-75

PMID number: 24859450

PMCID number: PMC4142638

Wang Y, Godec J, Ben-Aissa K, Cui K, Zhao K, Pucsek AB, Lee YK, Weaver CT, Yagi R, Lazarevic V. The transcription factors T-bet and Runx are required for the ontogeny of pathogenic interferon-γ-producing T helper 17 cells. Immunity. 2014 Mar 20;40(3):355-66.

PMID number: 24530058

PMCID number: PMC3965587

Williams LD, Amatya N, Bansal A, Sabbaj S, Heath SL, Sereti I, Goepfert PA. Immune activation is associated with CD8 T cell interleukin-21 production in HIV-1-infected individuals.

J Virol. 2014 Sep 1;88(17)

PMID number: 24942568

PMCID number: PMC4136345

Zajac AJ, Harrington LE. Tissue-resident T cells lose their S1P1 exit visas.

Cell Mol Immunol. 2014 May;11(3):221-3

PMID number: 24561454

PMCID number: PMC4085496

Useful Links

A 12 minute video introduction to flow cytometry: A must see for beginners :

Flow Cytometry – A Basic Introduction by Michael G. Ormerod

Practical Flow Cytometry --- Howard Shapiro

A useful Java applet put together by Joe Trotter of BD Biosciences. You can view the excitation and emission spectra of many common fluorescent dyes for help in panel design.

An applet similar to the above that has all of the Invtrogen reagents, as well as common fluorchromes

For FlowJo users: http://flowjo.typepad.com/ 

Excellent information on flow cytometry applications, especially intracellular staining

Invitrogen: The current home of the legendary Molecular Probes materials and lots of other useful information:

Cell Signaling

Millipore GUAVA

Intellicyt --- HyperCyt autosampler for high throughput flow cytometry (available in CFAR Core)

Contact Information

BBRB Location:

Director: Olaf Kutsch, PhD
Manager: Marion Spell, BS
E-mail: mlspell@uab.edu
Phone: (205) 975-3227

Bevill Basic Research Building
Room 557
845 19th Street South
Birmingham, Alabama, 35294

Shelby Location

Director: John D. Mountz, MD, PhD
Manager: Enid F. Keyser, BS
E-mail: efk@uab.edu
(205) 934-1362

Shelby Biomedical Research Building
Room 271
1825 University Blvd
Birmingham, Alabama, 35294