Assay Core


Directors:
Jim Collawn, Ph.D.
Catherine Fuller, Ph.D.

Established: 1994

Mission

The mission of the Assay Core is to develop and assist with protocols intended to evaluate macroscopic CFTR function and subcellular localization.


Research Information

The Core provides Ussing chamber capabilities and expertise for testing vectoral anion transport in polarized airway epithelial monolayers, and florescent dye-based methods for evaluating CFTR activity in cells grown on coverslips. The Core also performs immunolocalization for proteins relevant to CF pathogenesis, including CFTR.


Reagents and assays are funded by resources available from the Cystic Fibrosis foundation. Core users are typically associated with the Cystic Fibrosis Research Center, but there is no limitation on who may access the Core.

Contact information

Core Directors: Jim Collawn, Ph.D. or Catherine Fuller, Ph.D.
Phone: (205) 934-1002 or (205) 934-6227