Research Information

Established in 1994, The Center’s Assay Core works to develop and assist with protocols intended to evaluate CFTR function and expression, channel gating and regulation, and subcellular localization. The Core provides Ussing chamber and high throughput ion transport capabilities, florescent dye-based methods for evaluating CFTR activity, and a variety of patch clamp and excised patch assays. The Core also performs CFTR mRNA expression by digital and conventional real-time PCR technologies and immunolocalization for proteins relevant to CF pathogenesis, including CFTR.

Activities fall under three Specific Aims:
-Provide Ussing chamber capabilities and expertise for testing vectoral anion transport in polarized airway epithelial monolayers.
-Provide florescent dye-based methods for evaluating CFTR activity in cells grown on coverslips.
-Perform immunodetection and immunolocalization for proteins relevant to CF pathogenesis, including CFTR.

Contact Information

Core users are associated both within and beyond the CF Research Center. There is no limitation on who may access the Core. 

For additional information regarding resources and services, please contact:
James Collawn.png
James F. Collawn, Ph.D.
Director, Assay Core