Skin Proteomics Core
Stephen Barnes, Ph. D., Director
The overall goals of the proteomics core are:
- To provide high-resolution two dimensional electrophoretic and/or chromatographic protein separations coupled with MALDI-TOF and other mass spectrometry analyses; and
- To identify differences in polypeptide expression and/or modifications that may be the basis for various diseases of the skin.
The use of proteomics to identify novel proteins within the skin has received little attention. Yet this powerful technique can yield important new insights into the biology and pathology of skin. Dr. Eric Huang has already used proteomics and high resolution spectrometry to identify several new proteins within the epidermis.
Specific aims of the Proteomics Core include:
- Optimization of protein recovery from cells and tissue specimens
- High resolution protein separation
- Isoelectric focusing/SDS-PAGE electrophoresis (on three different 2nd dimension gel sizes).
- Qualitative and quantitative gel image analysis via software (currently PDQuest [BioRad]) to identify differential protein expression and/or alterations in posttranslational modifications
- MALDI-TOF mass spectrometric identification of polypeptides based on database searching with tryptic mass fingerprints
- NanoLC-electrospray ionization mass spectrometry for peptide sequence analysis and analysis of covalent modifications.
- Training in the experimental design and application of proteomics
- Use of protein databases and bioinformatics