Dept. of Biology
School of Natural Sciences & Mathematics
PhD, Biological Sciences, 1986
Albert Einstein College of Medicine, Bronx, NY
Postdoctoral Fellow 1986-90
Our laboratory is focused on understanding the physiological and energetic role of hydrogen sulfide (H2S), recently determined to have cell signaling functions in mammalian cells and tissues. H2S is analogous to nitric oxide (NO) and may even mediate NO signaling activity in the vascular and other systems. H2S may also play a role in a number of human pathologies. We investigate the role of H2S using methods to determine cell status and operation such as high resolution respirometry, optical spectrophotometry of intracellular endogenous and exogenously added optical probes, myography of intact blood vessels, luminometry of cellular ATP status, digital video microscopy with subarray scanning, and HPLC and enzymatic assays of metabolites. To establish the role of a labile molecule such as H2S in multiple cellular processes, it is critical to accurately determine the concentrations of H2S under both physiological and pathological conditions. To do this, we combine our newly developed polarographic hydrogen sulfide sensor (PHSS) with other methods to simultaneously follow real time kinetic changes in H2S and define the cellular processes that regulate H2S levels.
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