High-Field NMR Facility

Director

N. Rama Krishna, Ph.D. BMG, Chemistry & CCC
(205) 934-5695
Website: High Field NMR

Expertise

The UAB High-Field NMR facility offers expertise and assistance in the following research capabilities:

• Metabolite studies by in-vitro NMR on suspended cells, on biofluids (e.g., serum, blood plasma, urine etc), tissues, and cell and tissue extracts (homogenized extracts, perchloric acid extracts etc). We can implement a Metabonomics NMR capability if there is enough demand. This capability can complement imaging modalities.
• Quality control on synthetic and medicinal chemistry compounds by NMR of 1H and other nuclei (13C, 19F, 31P, 15N, 195Pt, etc.)
• Three-dimensional structure determinations of proteins and their complexes including complexes with low-molecular weight compounds
• STD-NMR screening of compound libraries to identify “hits” (an excellent complement to HTS screening method).
• Fragment-based discovery and design of new lead compounds
• Determination of binding epitopes of small molecules
• Structures of membrane proteins

Hardware

• Bruker Avance 700 MHz NMR system with a cryoprobe
• Bruker Avance 600 MHz NMR system with a cryoprobe
• Bruker Avance 500 MHz NMR system with a broadband probe

This Facility is also a Cancer Center Shared Facility