IDDRC Neurobiology Imaging and Tissue Processing Core-C
Director: Lucas Pozzo-Miller, Ph.D.
The Developmental Neurobiology Imaging and Tissue Processing Core provides state-of-the-art equipment and technical support for experimental projects on the assembly and modulation of synaptic, neuronal, and glial structure and function. By sharing technical expertise, equipment, facilities, and professional staff, the Core facilitates cost-effective, cross-project collaborations.
The objective of the Neurobiology Tissue Processing and Imaging Core (Core C) is to provide state-of-the-art equipment and technical support for a broad range of experimental projects on intellectual and developmental disabilities (IDD). By sharing technical expertise, equipment, facilities, and professional staff, this Core will facilitate cross-project collaborations among the different projects of the proposed IDDRC. Core C represents a “truly enabling” core service equipped and staffed to offer cytological and histological processing of experimental tissues, and perform image acquisition and analysis in a regular and reliable fashion for many of the projects outlined in this P30 IDDRC application. For us, “truly enabling” means that Core C provides both equipment and experienced staff that will assist IDDRC Investigators with all aspects of their projects, from initial development and experimental design to data acquisition and analyses, including training if needed. Often times, investigators are faced with the need to perform experiments that are outside their scope of expertise. Time-lapse multiphoton excitation microscopy and electron microscopy come to mind as examples. Investigators who rely extensively on these techniques may wish to learn the use of the equipment offered under the auspices of our highly experienced staff; they will then conduct experiments largely on their own. On the other end of the spectrum are investigators who have only incidental need for a particular technique and are quite content to have our staff conduct the experiments for them. We are in a position to assist in either instance, enabling investigators to accomplish technically difficult experiments that they would otherwise be unable to do. In addition, we seek to offer services regarding cell and tissue sectioning and staining processing. These services, while being a convenience for investigators, will have the added benefit of providing a reference set of tissues, cells, and protocols that can be shared by numerous investigators. This allows for collaborations between investigators each studying the same cells or tissues, making comparative analyses of data more feasible.
- A costum-made 2-photon microscope based on an Olympus FV300 confocal, a Zeiss 510 Meta confocal microscope, and a Zeiss digital fluorescence microscope with StereoInvestigator image analysis software.
- All necessary equipment for tissue processing for EM.