What types of samples do you accept?
We accept all kinds of samples. Some examples are: whole tissue, plasma, cell line, gel bands, purified protein, synthetic peptide, and formalin fixed tissue.
When shipping gel bands, what is the best solution for them to be in?
50mM AmBic.
What is the minimum amount of samples needed when doing a comparative study in order to receive a meaningful statistical analysis?
It is dependent upon whether you are using cell lines, animal models or human samples. Please contact Dr. Mobley, mobleyja@uab.edu , to get a more precise answer.
Who do I contact in order to find out who the samples need to be delivered to?
The lab manager, Brandon Young. His contact information is located at the bottom of the purchase order form.
What is the best way to bring over whole tissue?
The tissue should be snap frozen in liquid nitrogen.
When sending over gel bands, is there anything that should be brought along with it?
Yes. The two most important things are a picture of the gel before the bands were cut out and a blank gel band cut out from the gel to run alongside your samples.
Can you sequence proteins? What would be the cost? How long would this take?
Yes we can sequence proteins using the DeNovo sequencing approach. The cost can be found on our services webpage. For DeNovo sequencing it would take about a month for the whole process.
Regarding formalin fixed tissue, how many sections are needed and what approximate thickness should the tissue be?
We would need 2-4 sections at 5-8 micron thickness of similar size to have enough material from formalin fixed tissues.
Can you identify specific phosphorylated residues?