Pulmonary Proteomics Laboratory
Co-directors: Drs. Stephen Barnes and Helen Kim
Contact: Dr. J. Edwin Blalock, LHC Scientific Director (205-934-6439) or Dr. Patricia L. Jackson, LHC Liaison to PPL (205-934-3032)
- Provides LHC members access to the most up-to-date spectrometry/proteomics/metabolomics capabilities for applications to the study of chronic lung diseases.
- Applications to pulmonary biospecimens include, but are not limited to, biomarker discovery and quantitation, protein sequencing, analysis of protein abundance and/or modification differences between biospecimens, drug metabolism, lipidomics, metabolomics and peptidomics.
- The facility contains areas and computers for data processing by investigators and for teaching and consulting on issues involving mass spectrometry.
- The mass spectrometry component of PPL has instruments well suited for the analysis of post-translationally modified peptides and small molecules in biological fluids, tissues and complex mixtures. The 2D-proteomics component of the PPL offers multiple approaches for resolving and analyzing complex mixtures of proteins including conventional 2D-electrophoresis (IEF/SDS-PAGE), as well as difference gel electrophoresis (DIGE) using cydye-labeled proteins among others.
- Information about the applications of proteomics and mass spectrometry to biological research at UAB can be obtained at http://www.uab.edu/proteomics.