For information on our active clinical trials, please contact our office at:
(205) 934-7557 or (205) 934-7630.
The UAB Pulmonary Biospecimen Repository and P-TREC (Pulmonary Translational Research and Clinical Database)
The Lung Health Center's (LHC) Pulmonary Biospecimen Repository (PBR) (Dr. Joao deAndrade, Principal Investigator, Dr. Lisa Schwiebert, Director) offers LHC members access to biospecimen samples derived from multiple lung diseases. Specifically, the PBR contains fluids and cells isolated from the bronchoalveolar lavage, serum, and plasma of transplant, idiopathic pulmonary fibrosis, and COPD patients are available to LHC members and other investigators at UAB. Applications for pulmonary biospecimens include, but are not limited to, analyses of immune cell trafficking and signal transduction as well as inflammatory mediator expression. For further information and access to PBR resources, please contact Ms. Tonja Meadows, RN at (205) 934-7630 or fax the Collaboration Request Form to (205) 934-6229.
The core facility collects, stores, and assesses samples as described below:
Bronchoalveolar lavage (BAL) samples. Collected BAL samples are centrifuged to pellet the cellular fraction. Cell are counted and differentiated on cytospin preps using Diff-Quik stain. Upon request, cell differentials are then determined from at least 500 leukocytes using standard hematological criteria. Remaining cells are stored as cell pellets at -80oC for RNA, DNA, or protein analyses. Supernatants are stored at -80oC until distribution.
Serum and plasma samples. Collected blood samples are separated into serum and plasma. Each are ficolled and cells are harvested from buffy coat layers. Cells are then counted and differentiated on cytospin preps using Diff-Quik stain. Upon request, cell differentials are then determined from at least 500 leukocytes using standard hematological criteria. Remaining cells are stored as cell pellets at -80oC for RNA, DNA, or protein analyses. Serum and plasma fluid samples are stored at -80oC until distribution.
Sample organization and quality control. All samples are logged into the PBR using FreezerWorks, a state-of-the-art cataloging program.
designed for the organization of stored clinical samples. Cell and fluid samples are assessed routinely for viability. Specifically, cells are lysed and assayed for b-actin mRNA content via RT-PCR; fluids are examined for TNFa protein levels via ELISA.
Patient clinical history. Each sample is linked directly with the respective patient clinical history via P-TREC, a HIPPA compliant comprehensive clinical database developed by the Division of Pulmonary, Allergy and Critical Care Medicine and hosted by the Division of Preventive Medicine.