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CFCC at Shelby Equipment

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Please cite grant numbers:  NIH P30 AR048311 and NIH P30 AI27667

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 Dr. John Mountz, Director of the CFCC at Shelby, discusses the equipment available in a video that can be accessed by clicking here.

 Instrument

 Year Acquired

Source

Type Excitation Wavelength (nm)

 No. of Fluorescence Detectors

FACSAria II 2010 BD BioSciences Sorter 405, 488, 561, 640 14
LSRII 2006 BD BioSciences Analyzer  405, 488, 532, 635 18
FACSCalibur   BD BioSciences

Analyzer

488, 635 

4

FACScan 1988 (updated in 1997) BD BioSciences

Analyzer

488 3
AutoMACS 2006 Miltenyi MACS Sorter Not Applicable Not Applicable

 

 

 Instrument

 Year Acquired

Source

Type Number of Tubes

 Program Any Panel

SamplePrep Assistant 2006 BD
BioSciences
Robotic Sample Prep Up to 40 B cell, T cell, sub-setting, etc
Lyse Wash Assistant 2006 BD
BioSciences
Robotic Sample Prep Up to 40 B cell, T cell, sub-setting, etc
High Throughput Sampler (HTS) option on LSRII 2009 BD
BioSciences
Robotic Acquisition of Samples from 96- and 384-well plates N.A. Supports Immuno-phenotyping assays


Below are the specifications of the instruments available at the Wallace Tumor Institute (WTI), room 502

 

 Instrument

Type

Excitation Wavelength (nm)
Number of Fluorescence Detectors

Analysis Software

BD FacsSorter Analyzer 488nm
633nm
4 CellQuest
XP5FacScan Analyzer 488 nm
633 nm

5

FL1: FITC, GFP, CFSE
FL-2: PE, PI
FL-3: PerCP, PE-Cy5, PE-Cy5.5
FL-4: APC
FL-5: APC-Cy7

FlowJo Collector's Edition Acquisition Software

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