Equipment & Software

Thermo Fisher Orbitrap Velos Pro

Agilent 1260 Infinity nano-HPLC stack and & Auto-sampler


Thermo Fisher LTQ-XL

Dionex Ultimate 3000 UHPLC+ Focused Stack & Auto-sampler


Thermo Fisher LTQ-XL with ETD

Surveyor MS HPLC Stack & Auto-sampler


Bruker Ultraflex III MALDI ToF/ToF


Shimadzu ChIP-1000 (HD Chemical Printer for Imaging-MS)


GE Ettan™ IPGphor IEF System

Large Format BioRad Protean® Plus Dodeca Cell w/ Power Supply

Amersham Multitemp III w/ Circulating Pump


GE Ettan™ Spot Picker


BioTek Microplate Spectrophotometer

(Synergy 2 Multi-Mode UV/ VIS/ / NIR/ FL/ L Top& Bottom Reader)


UVP BioSpectrum® Imaging System (UV/ VIS/ IR/ FL/ L)


Shandon Cryotome™ FSE Cryostat w/ Histology Station


Sutton Nano Laser Quartz Tip Puller w/ column packing bomb


GE nanoCOLLECT System (LC-MALDI-MS Spotter)

Surveyor MS HPLC Stack & Auto-sampler


Shimadzu (analytical/ preparative) HPLC w-UV/Vis Detector

Gilson Fraction Collector


Cell Culture Room: Laminar Flow Hood, CO2 Incubator, & 2 Microscopes


Two -80oC Refrigerators (Long-Term Storage Available For MSP-SF Users)


Server Stack

Our facility houses nearly 30 computers that include 2 high-end workstations and 5 high-speed high capacity double firewalled servers(below) that contain 40TB of dynamic drive space and an additional 60TB of backup storage for archiving and automated nightly backups for all computers throughout the MSP-SF (including all MS instruments).


  • Primary fileserver: (30TB of drive space, 32 GB RAM, 2 . AMD 3.1 GHz 8 Core processors)
  • Unix application server: powered by an Oracle Database (64 GB RAM, 2 . AMD 2.6GHz 12 Core processors)
  • Primary domain controller: for our network (2 GB RAM, 1 . Intel 2.00 GHZ Dual Core processor)
  • Secondary fileserver: (4 GB RAM, 2 . Intel 2.00 GHz Dual Core Processors)
  • Application server: (32 GB RAM, 2 . AMD 2.2GHZ 12 Core processors)

The primary fileserver is also dynamically linked to a 2000 node Cluster - UAB Shared High Performance Computing Facility. (https://docs.uabgrid.uab.edu/wiki/Resources)


Software:

  • Search Engines: SEQUEST, MASCOT
  • Denovo Sequencing: MASCOT Distiller
  • 2D PAGE Analysis: Nonlinear Samespots
  • MS Peptide & PTM Grouping, Filtering, and Quantification Analysis: MASCOT Distiller Q+, Proteome Software Scaffold, Scaffold PTM/ Q+S
  • Bruker Daltronics Suite: LCWARP, Biotools, Rapid Denovo, Fleximaging
  • Statistical Analysis: Matlab, Statistica, SPSS, Graphpad, Origin
  • Systems Analysis: GeneGO-metaCore, Blast2Go-Pro
  • Data Sharing: MSP-SF LIMS, all data is retrievable via HTML

Additional Essential Equipment:

  • Small Molecule & Lipid Preparation Station (including two multi-well SPE devices w/ multi-well heated drying system – w/ argon & nitrogen)
  • Chemical Synthesis & Distillation Apparatus
  • Savant Explorer SpeedVac® Concentrator with multiple adapters including 96 deep well
  • Sorvall Legend XTR centrifuge with multiple adapters including a 96 deep well plate holder
  • Chemical Fume Hoods (2)
  • Refrigerated Cold Cabinets (2)
  • Freezers -20°C (2)
  • Multiple Centrifuges (cooled and RT)
  • Multiple Standard and Midi gel boxes for 1D PAGE & WB
  • 1D Gel Electro-Eluter
  • Thermocyclers (3)
  • High-Speed 96 Multi-Well Shaker
  • Heated Multi-Well Shaker
  • Large Water Bath Sonicator
  • Cell Disruptor