Advanced Graduate Course 2009
(BMG 744)

The science of proteomics and mass spectrometry

Course will be held on Tuesdays and Fridays 9 - 11 am in the McCallum Building, 4th floor conference room (401)


Date Lecturer Topic
Jan 6, TuS. Barnes/H. Kim The world of biomolecules. The proteome, proteomics and other –omics and where to start [PDF File] [Questions]
Jan 9, FriM. Renfrow Mass spectrometry – gas phase transfer and instrumentation – include ETD [PDF File] [Questions]
Jan 13, Tu S. Barnes Methods for the identification of proteins: MALDI-TOF of proteins and peptide mass fingerprinting; LC analysis and peptide sequencing
[PDF File] [Questions]
Jan 16, Fri S. Barnes Ion fragmentation in mass spectrometry; application to proteomics
[PDF File] [Questions]
Jan 20, Tu J. Prasain Ion Fragmentation of small molecules [PDF File] [Questions]
Jan 23, Fri S. Barnes/
E Shonsey
Sample preparation for proteomics and mass spectrometry [PDF File] [Questions]
MS in Forensics [PDF File]
 Mid-term exam – take-home
Jan 27, TuA. SmithConnecting proteomics into bioinformatics; MUDPIT and SEQUEST; false discovery rates in complex systems
Jan 30, Fri C. Crasto The bioinformatics of the proteome [PDF File]
Feb 3, Tu S. Barnes/H. Kim Simplifying the proteome - techniques of protein purification; Protein separation by electrophoresis and other 2D-methods [PDF File] [Questions]
Feb 6, FriJ. Prasain The use of mass spectrometry in lipidomics [PDF File] [Questions]
Feb 10, TuS. BarnesMass spectrometry in qualitative and quantitative burrowing of the proteome [PDF File] [Questions]
Feb 13, FriJ. PrasainQualitative and quantitative analysis/method validation in metabolomics [PDF File] [Questions]
Feb 17, TuS. BarnesEnzymology and mass spectrometry [PDF File]
Feb 20, FriM. RenfrowAnalysis of protein-protein interactions by affinity purification and mass spectrometry [PDF File 1] [PDF File 2] [PDF File 3]
Feb 24, TuP. PreveligeMass Spectrometry as a Tool for Studying Protein Structure
Feb 27, FriP. PreveligeStudy of macromolecular structures – protein complexes
Mar 3, TuC-C. WangTissue and body fluid proteomics and mass spectrometry
Mar 6, FriK. ScheyApplications of MS to tissue imaging
Mar 10, TuM. RenfrowApplications of FT-ICR-MS
Mar 13, FriJ. Novak/
M. Renfrow
Mass spectrometry in glycomics research - Application to IgA nephropathy [PDF File]
Mar 17, TuH. KimUse of proteomics and MS methods in the study of the brain proteome and neurodegenerative diseases [PDF File]
Mar 20, FriS. BarnesIsotopes in mass spectrometry
 Final report due