Classes

Advanced Graduate Course 2011
(BMG/PHR 744)

The science of proteomics and mass spectrometry

Course Description

Course will be held on Mondays, Wednesdays and Fridays 9 – 10:30 am in the McCallum Building, 4th floor conference room (401)

Schedule

Date Lecturer Topic
  Basics Course
Jan 5, Wed S. Barnes/H. Kim The world of biomolecules. The proteome, proteomics and other –omics and where to start [PDF File] [Questions]
Jan 7, Fri M. Renfrow Mass spectrometry – gas phase transfer and instrumentation – include ETD [PDF File]
Youtube Movies[Quadrupole, FT-ICR MS, Triple Quad, Synapt G2 (ion mobility)]
Jan 12, Wed M. Renfrow Methods for the identification of proteins: MALDI-TOF of proteins and peptide mass fingerprinting; LC analysis and peptide sequencing [PDF File]
Jan 14, Fri M. Renfrow Ion fragmentation in mass spectrometry; application to proteomics [PDF File]
Jan 17, Mon S. Barnes Isotopes in mass spectrometry [PDF File]
Jan 18, Tue,
8-9:30 am
(Re-scheduled)
H. Kim Simplifying the proteome - techniques of protein purification [PDF File]
Jan 19, Wed J. Prasain Ion fragmentation of small molecules; lipidomics [PDF File]
Jan 21, Fri S. Asmellash Sample preparation for proteomics and mass spectrometry [PDF File]
Jan 24, Mon S. Barnes Mass spectrometry in qualitative and quantitative burrowing of the proteome [PDF File]
Jan 26, Wed J. Mobley Connecting proteomics into bioinformatics; MUDPIT and SEQUEST; false discovery rates in complex systems
Jan 28, FriC. Crasto The bioinformatics of the proteome; web tools; MRMPath [PDF File]
Jan 31, Mon  Exam
  Applications Course
Feb 2, Wed S. Barnes Course introduction [PDF File]
Feb 4, Fri J. Prasain Designing the metabolomics experiment [PDF File]
Feb 7, Mon J. Prasain Qualitative and quantitative analysis/method validation in metabolomics [PDF File]
Feb 9, Wed H. Kim Protein separation by electrophoresis and other 2D-methods
Feb 11, Fri S. Barnes Enzymology and mass spectrometry [PDF File]
Feb 14, Mon  Student presentations
Feb 16, Wed M. Renfrow Analysis of protein-protein interactions by affinity purification and mass spectrometry
Feb 18, Fri P. Prevelige Mass Spectrometry as a Tool for Studying Protein Structure
Feb 21, Mon P. Prevelige Study of macromolecular structures – protein complexes
Feb 25, Fri D. Stella Applications of MS to tissue imaging [PDF File] [PPT File]
Feb 28, Mon   Student presentations
Mar 2, Wed J. Mobley Tissue and body fluid proteomics and mass spectrometry [PDF File] [PPT File]
Mar 4, Fri M. Renfrow Applications of FT-ICR-MS
Mar 7, Mon J. Novak/
M. Renfrow
Mass spectrometry in glycomics research - Application to IgA nephropathy
Mar 9, Wed H. Kim Use of proteomics and MS methods in the study of the brain proteome and neurodegenerative diseases [PDF File]
Mar 14, Mon H. Kim/S. Barnes Putting it all together – by-passing pyruvate kinase
Mar 16, Wed E. Shonsey MS in Forensics [PDF File] [PPT File]
Mar 25, fri   Final report due