Classes

Advanced Graduate Course 2010
(BMG/PHR 744)

The science of proteomics and mass spectrometry

Course Description

Course will be held on Tuesdays and Fridays 9 - 11 am in the McCallum Building, 4th floor conference room (401)

Provisional schedule

Date Lecturer Topic
Jan 5, TuS. Barnes/H. Kim The world of biomolecules. The proteome, proteomics and other –omics and where to start
Jan 8, FriM. Renfrow Mass spectrometry – gas phase transfer and instrumentation – include ETD
Jan 12, Tu S. Barnes Methods for the identification of proteins: MALDI-TOF of proteins and peptide mass fingerprinting; LC analysis and peptide sequencing
Jan 15, Fri S. Barnes Ion fragmentation in mass spectrometry; application to proteomics
Jan 19, Tu S. Barnes Isotopes in mass spectrometry
Jan 22, Fri J. Prasain Ion fragmentation of small molecules; Lipidomics
Jan 26, Tu S. BarnesSample preparation for proteomics and mass spectrometry
Jan 29, Fri E. Shonsey MS in Forensics
Mid-term exam – take-home
Feb 2, Tu H. Kim Simplifying the proteome - techniques of protein purification; protein separation by electrophoresis and other 2D-methods
Feb 5, FriA. Smith IV Connecting proteomics into bioinformatics; MUDPIT and SEQUEST; false discovery rates in complex systems
Feb 9, TuC. CrastoThe bioinformatics of the proteome
Feb 12, FriJ. PrasainDesigning the proteomics and metabolomics experiment
Feb 16, TuS. BarnesMass spectrometry in qualitative and quantitative burrowing of the proteome
Feb 19, FriJ. PrasainQualitative and quantitative analysis/method validation in metabolomics
Feb 23, TuS. BarnesEnzymology and mass spectrometry
Feb 26, FriM. RenfrowAnalysis of protein-protein interactions by affinity purification and mass spectrometry
Mar 2, TuP. PreveligeMass spectrometry as a tool for studying protein structure
Mar 5, FriP. Prevelige Study of macromolecular structures - protein complexes
Mar 9, TuC-C. WangTissue and body fluid proteomics and mass spectrometry
Mar 12, Fri J. Mobley/
D. Stella
Applications of MS to tissue imaging
Mar 16, TuM. RenfrowApplications of FT-ICR-MS
Mar 19, Fri J. Novak/
M. Renfrow
Mass spectrometry in glycomics research - application to IgA nephropathy
Mar 23, Tu H. KimUse of proteomics and MS methods in the study of the brain proteome and neurodegenerative diseases
 Final report due