Helpful Links
Societies
The American Society for Mass Spectrometry (
http://www.asms.org) will celebrate its 57th National Meeting in Philadelphia, Pennsylvania, May 31 - June 4, 2009. The deadline for submitting abstracts is February 6, 2009.
Sites for Data Analysis
There are several sites on the Internet to get useful programs and information
for those doing protein mass spectrometry.
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Protein identification from tryptic peptide fingerprint data from a MALDI-TOF
analysis, or peptide mass and fragmention data from a triple quadrupole
instrument or a Q-tof, the best sites are:
http://www.matrixscience.com
(the MASCOT program)
http://donatello.ucsf.edu/
(ProteinProspector)
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A procedure for the in-gel digestion of proteins separated by 1D SDS-PAGE
analysis or 2D-IEF/SDS-PAGE is well described at http://donatello.ucsf.edu/ingel.html
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For those who use this procedure or have carried out digestion with proteolytic
enzymes in solution, the resulting peptides can be identified using UCSF MS
Fit
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Once a potential match has been made, MS Digest can be used to predict
all potential peptides including user-specified posttranslational modifications.
UCSF MS Digest
- 2D-proteomics databases
are becoming available for many cell types and organs/tissues from several
species as well as subcellular fractions. These are frequently annotated
with regard to spot identification. The EXPASY site contains links to
these databases as well as other useful programs and software for mass
spectrometry http://www.expasy.org/ch2d/
Journals
Other useful sites
