Welcome to Targeted Metabolomics and Proteomics Laboratory

Improved high sensitivity analysis of polyphenols and their metabolites by nano-liquid chromatography–mass spectrometry

Analysis of isoflavones and their metabolites by nanoLC-MS/MS on an AB Sciex 5600 TripleTOF both increases the sensitivity of the method allowing investigation of small amounts of starting material. This approach, where the mass windows are selected AFTER the collection of MS and MSMS data, can be applied to any xenobiotic (drug, food or environmental toxin) and/or their metabolite(s). In figure A, the signal for the isoflavone genistein in a urine extract is hard discern when the mass window is 0.7 m/z; however, narrowing of  the mass window to 0.05 m/z completely isolates the genistein peak.

Dr. Stephen Barnes and the TMPL team recently published this work in Archives of Biochemistry and Biophysics 2014;559:3-11. [doi: 10.1016/j.abb.2014.06.014]  [PMCID: PMC4143533]

See full publication list.

3rd Annual Workshop on Metabolomics

June 14-18, 2015

The 3rd Annual Workshop on Metabolomics will be held June 14-18 2015 on the campus of the University of Alabama at Birmingham.

The course is jointly sponsored by the National Institute of General Medical Sciences (NIGMS) as part of the NIH Common Fund Metabolomics Initiative, and the Department of Pharmacology and Toxicology at UAB.

Go to Metabolomics Workshop web page.


MRM
Figure from J Chromatogr B Analyt
Technol Biomed Life Sci.
[PMID: 20346741]
TMPL Movie
PPT file

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About TMPL

The Targeted Metabolomics and Proteomics Laboratory (TMPL) is organized to provide a variety of analytical and technical services using mass spectrometry to UAB investigators. The laboratory has three mass spectrometers, a triple quadrupole instrument (AB Sciex 4000), a quadrupole-linear ion trap instrument (AB Sciex 6500 Qtrap), and quadrupole-TOF (AB Sciex 5600 TripleTOF). The AB Sciex 5600 TripleTOF is particularly powerful for comprehensive and targeted proteomics, lipidomics and metabolomics. The combination of Eksigent microflow 200 LC and 415 nanoLC with the mass spectrometers provides the highest possible sensitivity for sample analysis.

Two fourier transform-ion cyclotron resonance instruments - supported by NCRR grants - are in the Biomedical FT-ICR Mass Spectrometry Laboratory (http://www.uab.edu/BiomedFTICR/). Contact Dr. Matt Renfrow about use of these instruments (renfrow@uab.edu; 205 996-4681).

A Bruker MALDI TOF-TOF instrument is available in the Comprehensive Cancer Center Core Laboratory of Dr. Jim Mobley (mobleyja@uab.edu; 205 996-6363).

New sample and data-handling arrangements in TMPL – investigators please note