Welcome to Targeted Metabolomics and Proteomics Laboratory

Modification of platelet proteins by 4-hydroxynonenal: Potential Mechanisms for inhibition of aggregation and metabolism. [PMID: 26475426]

In this paper from the laboratory of Dr. Victor Darley-Usmar, the TMPL group was able to provide both information on glycolytic and Krebs cycle intermediates using targeted, multiple reaction ion monitoring LC-MS as well as on the modification of proteins in platelets by 4-hydroxynonenal using nanoLC-MS/MS. 4HNE is a factor in many diseases such as renal failure, septic shock and cardiopulmonary bypass surgery. The importance of the Krebs cycle and its intermediates in many other diseases including cancer is increasingly being appreciated.

Contact Taylor Berryhill (205 934–3462, berrytf@uab.edu) for more information on the the analysis of Krebs cycle and other intermediates
Contact Landon Wilson (205 934-3462, empy1977@uab.edu) for more information identification of sites of protein modifications

See full publication list.

3rd Annual Workshop on Metabolomics

June 14-18, 2015

The 3rd Annual Workshop on Metabolomics was held June 14-18 2015 on the campus of the University of Alabama at Birmingham.

The course is jointly sponsored by the National Institute of General Medical Sciences (NIGMS) as part of the NIH Common Fund Metabolomics Initiative, and the Department of Pharmacology and Toxicology at UAB. It featured special seminars by Dr. Richard Caprioli (Vanderbilt University), Art Edison (University of Florida) and David Wishart (University of Alberta). These seminars and the rest of the workshop will be made available over the next several months. Please revisit this site to view these materials.

Go to Metabolomics Workshop web page.

Figure from J Chromatogr B Analyt
Technol Biomed Life Sci.
[PMID: 20346741]
TMPL Movie
PPT file

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About TMPL

The Targeted Metabolomics and Proteomics Laboratory (TMPL) is organized to provide a variety of analytical and technical services using mass spectrometry to UAB investigators. The laboratory has three mass spectrometers, a triple quadrupole instrument (AB Sciex 4000), a quadrupole-linear ion trap instrument (AB Sciex 6500 Qtrap), and quadrupole-TOF (AB Sciex 5600 TripleTOF). The AB Sciex 5600 TripleTOF is particularly powerful for comprehensive and targeted proteomics, lipidomics and metabolomics. The combination of Eksigent microflow 200 LC and 415 nanoLC with the mass spectrometers provides the highest possible sensitivity for sample analysis.

Two fourier transform-ion cyclotron resonance instruments - supported by NCRR grants - are in the Biomedical FT-ICR Mass Spectrometry Laboratory (http://www.uab.edu/BiomedFTICR/). Contact Dr. Matt Renfrow about use of these instruments (renfrow@uab.edu; 205 996-4681).

A Bruker MALDI TOF-TOF instrument is available in the Comprehensive Cancer Center Core Laboratory of Dr. Jim Mobley (mobleyja@uab.edu; 205 996-6363).

New sample and data-handling arrangements in TMPL – investigators please note