Welcome to Targeted Metabolomics and Proteomics Laboratory

Teaching metabolomics: a UAB outreach to researchers in sub-Saharan Africa

by Jeff Hansen

Janusz Kabarowski and SteveBarnes

For the last 10 weeks, Stephen Barnes, Ph.D., University of Alabama at Birmingham, has taught a group of master’s degree students at a university in the land-locked African nation of Mali. His outreach — via two hours of video conferencing each day and a lot of class material preparation — came at the request of the National Institute of Allergy and Infectious Diseases, to help NIAID create a cadre of bioinformatics researchers at one of the ground zeros for the deadly infectious disease malaria.

Read more at UAB News.

Inhibition of autophagy with bafilomycin and chloroquine decreases mitochondrial quality and bioenergetic function in primary neurons.

Redmann M, Benavides GA, Berryhill TF, Wani WY, Ouyang X, Johnson MS, Ravi S, Barnes S, Darley-Usmar VM, Zhang J. Redox Biol. 2017 Apr;11:73-81. [doi: 10.1016/j.redox.2016.11.004] Epub 2016 Nov 18. PMID:27889640

See full publication list.

Lower Levels of Cervicovaginal Tryptophan are Associated with Natural Clearance of Chlamydia in Women.

Jordan SJ, Olson KM, Barnes S, Wilson LS, Berryhill TF, Bakshi R, Brown LT, Press CG, Geisler WM. J Infect Dis. 2017 May 17. doi: 10.1093/infdis/jix240. [Epub ahead of print] [doi: 10.1093/infdis/jix240] [PMID:28520912]

5th Annual Workshop on Metabolomics

July 16-21, 2017

The 5th Annual Workshop on Metabolomics will be held July 16-21, 2017 on the campus of the University of Alabama at Birmingham.

The course is jointly sponsored by the National Institute of General Medical Sciences (NIGMS) as part of the NIH Common Fund Metabolomics Initiative, and the Departments of Chemistry and Pharmacology and Toxicology at UAB.

Details on the workshop can be found on the Metabolomics Workshop web page.


MRM
Figure from J Chromatogr B Analyt
Technol Biomed Life Sci.
[PMID: 20346741]
TMPL Movie
PPT file

MRMPath

About TMPL

The Targeted Metabolomics and Proteomics Laboratory (TMPL) is organized to provide a variety of analytical and technical services using mass spectrometry to UAB investigators. The laboratory has three mass spectrometers, a triple quadrupole instrument (AB Sciex 4000), a quadrupole-linear ion trap instrument (AB Sciex 6500 Qtrap), and quadrupole-TOF (AB Sciex 5600 TripleTOF). The AB Sciex 5600 TripleTOF is particularly powerful for comprehensive and targeted proteomics, lipidomics and metabolomics. The combination of Eksigent microflow 200 LC and 415 nanoLC with the mass spectrometers provides the highest possible sensitivity for sample analysis.

Two fourier transform-ion cyclotron resonance instruments - supported by NCRR grants - are in the Biomedical FT-ICR Mass Spectrometry Laboratory (http://www.uab.edu/BiomedFTICR/). Contact Dr. Matt Renfrow about use of these instruments (renfrow@uab.edu; 205 996-4681).

A Bruker MALDI TOF-TOF instrument is available in the Comprehensive Cancer Center Core Laboratory of Dr. Jim Mobley (mobleyja@uab.edu; 205 996-6363).

New sample and data-handling arrangements in TMPL – investigators please note