Welcome to Targeted Metabolomics and Proteomics Laboratory
GBSC 724 - The Metabolome and its Importance in Biomedical Research
This course will be held on Mondays, Wednesdays and Fridays from 9-10:30 am and starts on Monday, January 8, 2018.
Why study the metabolome? The metabolome is not just the integration of metabolic pathways generated by the products of our genes, but also all the chemicals that enter our bodies, and/or the models you use in the lab. It provides more information than genomics, transcriptomics and proteomics, and provides a rationale for the regulation of epigenetics.
This course provides understanding of where the chemical complexity of life comes from, how to study it in the context of the experiments you do, how to measure the metabolome, and how to make sense of the results. http://www.uab.edu/proteomics/massspec/classes/schedule.php
Recent publications from TMPL
Comprehensive profiling of prostaglandins in human ovarian follicular fluid using mass spectrometry. Pier B, Edmonds JW, Wilson L, Arabshahi A, Moore R, Bates GW, Prasain JK, Miller MA. Prostaglandins Other Lipid Mediat. 2017 Nov 9. [doi: 10.1016/j.prostaglandins.2017.11.001] [Epub ahead of print] [PMID: 29129796]
Glutaminolysis Promotes Collagen Translation and Stability via α-ketoglutarate Mediated mTOR Activation and Proline Hydroxylation. Ge J, Cui H, Xie N, Banerjee S, Guo S, Dubey S, Barnes S, Liu G. Am J Respir Cell Mol Biol. 2017 Oct 11. doi: 10.1165/rcmb.2017-0238OC. [Epub ahead of print] [PMID:29019707]
Detailed Investigation and Comparison of the XCMS and MZmine 2 Chromatogram Construction and Chromatographic Peak Detection Methods for Preprocessing Mass Spectrometry Metabolomics Data. Myers OD, Sumner SJ, Li S, Barnes S, Du X. Anal Chem. 2017 Sep 5;89(17):8689-8695. doi: 10.1021/acs.analchem.7b01069. Epub 2017 Aug 17. [PMID: 28752757]
|Figure from J Chromatogr B Analyt
Technol Biomed Life Sci.
The Targeted Metabolomics and Proteomics Laboratory (TMPL) is organized to provide a variety of analytical and technical services using mass spectrometry to UAB investigators. The laboratory has three mass spectrometers, a triple quadrupole instrument (AB Sciex 4000), a quadrupole-linear ion trap instrument (AB Sciex 6500 Qtrap), and quadrupole-TOF (AB Sciex 5600 TripleTOF). The AB Sciex 5600 TripleTOF is particularly powerful for comprehensive and targeted proteomics, lipidomics and metabolomics. The combination of Eksigent microflow 200 LC and 415 nanoLC with the mass spectrometers provides the highest possible sensitivity for sample analysis.