Grants
Recent awards involving the Mass Spectrometry/Proteomics Shared Facility
- The National Center for Research Resources (NCRR) in 2010 awarded a $469,212 Shared Instrumentation grant to Dr. Stephen Barnes, Director of the Mass Spectrometry Shared Facility, for a 5500 Q-trap Mass Spectrometer S10RR027822-01
- The UAB Health Services General Endowment Fund award $363,350 to Dr. Stephen
Barnes, Director of the Mass Spectrometry Shared Facility towards the purchase
of ABI-Sciex 4000 and ABI-Sciex 3200 triple quadrupole mass spectrometers.
These replace trhe API-3 triple quadrupole instrument purchased in 1993.
- The UAB Health Services General Endowment Fund awarded $200,000 in 2006 to
Dr. Stephen Barnes, Director of the Mass Spectrometry Shared Facility, towards
the purchase of a MALDI-TOF-TOF mass spectrometer for high throughput peptide
tandem mass spectrometry and for tissue imaging mass spectrometry. Contact
Dr. Jim Mobley (205-934-1470; mobleyja@uab.edu) for information about the use of this instrument.
- The National Center for Research Resources (NCRR) in 2004 awarded a $465,000 Shared Instrumentation grant to Dr. Stephen Barnes, Director of the Mass Spectrometry Shared Facility, for a triple quadrupole mass spectrometer. An ABI-Sciex 4000 Qtrap was purchased with these funds.
- The National Center for Research Resources (NCRR) in 2002 awarded a $1,511,500
Shared Instrumentation grant to Dr. Stephen Barnes, Director of the Mass Spectrometry
Shared Facility, for the purchase of a high field FT-ICR mass spectrometer.
These funds were used to purchase two Thermofinnigan LTQ-FT-ICR-MS instruments.
These are part of the UAB Laboratory for Biomedical FT-ICR-MS. Contact Dr.
Matthew Renfrow (205-996-4681; renfrow@uab.edu)
for information about the use of these instruments.
- The National Center for Research Resources (NCRR) in 2002 awarded a $446,320 Shared Instrumentation grant to Dr. Helen Kim, Director of the 2D-Proteomics Laboratory, for the purchase of an integrated and robotic system for high throughput image analysis of 2D-gels, robotic gel spot excision, and in-gel digestion and spotting onto mass spectrometry target plates. Genomic Solution robots were purchased and are utilized with three types of scanners (BioRad densitometer, Perkin-Elmer ProExpress for fluorescence and Odyssey for infra-red analysis) and two image analysis systems (NonLinear Dynamics Progenesis and GE Healthcare DeCyder).
Acknowledgements
When writing manuscripts or reports, please make sure to acknowledge the origins of grant support for the Mass Spectrometry Shared Facility and its instruments. Purchase of the mass spectrometers in the Targeted Metabolomics and Proteomics Laboratory came from funds provided by the following NCRR grants:
- Perceptive Biosystems DE-Pro MALDI-TOF - S10 RR13795 plus UAB Health Services Foundation General Endowment Fund
- Micromass Q-tof - S10 RR13795 plus UAB Health Services Foundation General Endowment Fund
- Thermo Finnigan LTQ FT-ICR S10 RR17261
- ABI-Sciex 4000 Qtrap S10 RR19231
Funds for the operation of the Targeted Metabolomics and Proteomics Laboratory come in part from the UAB Center for Nutrient-Gene Interaction (U54 CA 100949), the Purdue-UAB Botanicals Center for Age-Related Disease (P50 AT00477), the UAB O'Brien Acute Kidney Injury Center (P30 DK079337), the UAB Skin Disease Research Center (P30 AR50948), and the UAB Lung Health Center. We also acknowledge NIH ARRA funds to Dr. Moon Nahm that were used to upgrade the performance of the DE-Pro in 2009.