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2D-Proteomics/Mass Spectrometry Questionnaire

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* Name:
* Department:
* Phone #:
* Email:

1. Have you used this shared facility:

i. In the past 12 months	Yes No
ii. In the past 5 years	Yes No
iii.Never	Yes No

2. Which of the following do you analyze/investigate (check each relevant box):

3. Which of the 1D- and 2D-electrophoresis methods below are used for your research? Indicate in the third box whether you need assistance/training in these technologies.

4. Sample preparation is essential for successful 2D-proteomics and any form of mass spectrometry. Which of the following apply:

Already have a working method for proteins that has produced good results	Yes No
The method(s) used in my lab always seems to identify keratins and structural proteins	Yes No
Never tried preparing samples for 2D-proteomics or mass spectrometry	Yes No
Interested in developing an optimal method	Yes No

5. Quantitative analysis is what I need for my research. I'm interested in the following:

6. Please indicate which of the following training workshops would be of most value to you. Rank each on a scale from 1-4, with 1 being the most important.

1D-SDS PAGE	Yes No	Training
1D-SDS-PAGE with tricine buffers	Yes No	Training
1D-native PAGE	Yes No	Training
1D-isoelectricfocusing	Yes No	Training
2D-isoelectric focusing/SDS-PAGE	Yes No	Training
2D-isoelectric focusing/SDS-PAGE with Cy-dyes	Yes No	Training
2D-blue native electrophoresis	Yes No	Training
2D-clear native electrophoresis	Yes No	Training

Sample preparation for 1D- and 2D-electrophoresis applications	1 2 3 4
Sample preparation for peptide mass fingerprinting and nanoLC-tandem mass spectrometry	1 2 3 4
Database searching (MASCOT and SEQUEST) for identifying proteins	1 2 3 4
Interpreting tandem mass spectra	1 2 3 4
Selecting peptide and fragment ions for quantitative analysis of peptides and proteins	1 2 3 4
Qualitative and quantitative analysis of small molecules (drugs, drug metabolites, xenobiotics, dietary factors, endogenous compounds)	1 2 3 4

Proteins (recombinant)	Yes No
Proteins (enzyme preps)	Yes No
Proteins (organs, e.g., liver, brain, muscle)	Yes No
Proteins (cells)	Yes No
Proteins (subcellular organelles)	Yes No
Proteins (complexes)	Yes No
Proteins (Western blotting)	Yes No
Proteins (affinity pulldowns)	Yes No
Proteins (phosphorylated)	Yes No
Proteins (other post-translationally modified)	Yes No
Proteins (body fluids –e.g., blood, sweat and tears)	Yes No
Drugs (prodrugs, drugs and metabolites)	Yes No
Xenobiotics (DES, phthalates, chlorinated compounds)	Yes No
Dietary components( bioflavonoids, vitamins)	Yes No
Endogenous (bile acids, steroids, lipids, peptides)	Yes No

Peptides by iCAT and LC-tandem mass spec	Yes No
Peptides by iTRAQ and LC-tandem mass spec	Yes No
Peptides by multiple reaction ion monitoring mass spectrometry	Yes No
Peptides by isotope dilution-mass spectrometry	Yes No
Cy-dye labeling of proteins	Yes No
Small molecule (drug, exogenous and endogenous) by multiple reaction ion monitoring mass spectrometry	Yes No

UAB Targeted Metabolomics and Proteomics Laboratory

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2D-Proteomics/Mass Spectrometry Questionnaire