Transgenic Mouse Facility (TMF)

Director: Bob Kesterson, PhD

Coordinator: Larry Johnson

Department/Center Association: Genetics/Comprehensive Cancer Center, UAB Arthritis and Musculoskeletal Center, Recessive Polycystic Disease Core Center

Established: 1991

Mission

The Transgenic Mouse Facility (TMF) provides a centralized facility for the efficient production of genetically modified or transgenic mouse models.

Facility Description

Located in the Kaul Building, TMF services include the production of mouse models using DNA microinjection and embryonic stem (ES) cell transfer techniques. More than 5,000 founder animals (transgenic and ES cell germ-line chimeras) have been produced by the facility since its inception. Additional services include: complete mouse ES cell gene targeting experiments; embryo cryopreservation; rederivation of pathogen-free rodents; and assisted reproduction techniques such as in vitro fertilization, superovulation, rederivation and embryo transfer.

Research Information

Fees for research services are as follows:

· production of transgenic mice by oocyte microinjection ($1900)

· gene targeting of ES cells ($3,300 – investigator responsible for screening)

· gene targeting of ES cells ($3,800 – TMF responsible for screening)

· blastocyst microinjection of ES cells and transfer to pseudopregnant females ($1800/day or 40 blastocysts).

Charges for other services will be prearranged by the Director and will be formulated on a cost recovery basis.

Contact Information

Coordinator: Larry Johnson

Email: lwj@uab.edu

Phone: 205-934-2998

Web Site: http://www.genetics.uab.edu/Transgenics/index.htm

Approved by: Bob Kesterson, PhD, Director

Date: April 15, 2008

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