The Flow Cytometry Facility provides state-of-the-art cell sorting and analytical flow cytometry services to support research programs. Most services are provided at one of two convenient locations on campus. Training, consultation, assisted- and self-serve equipment usage is available.

Locations

cfarcoreBevill Biomedical Research Building Location

  • Newest Instrumentation
    • BD Accuri C6 Flow Cytometer
  • Cell sorting
    • BD FACS Aria
  • Cell analysis
    • BD LSR II GUAVA EasyCyte
  • Bioplex analysis
  • High throughput flow cytometry
    • Hypercyt autosampler
apcfcoreShelby Biomedical Building Location

  • Cell sorting
    • BD FACS Aria
  • Cell analysis
    • BD LSR II
    • FACS Calibur
    • FACS Scan
  • AutoMacs
    • MACS Sorter

 

Scheduling


First Time Users:
Contact Blake Glover Blake.glover@uabmc.edu to be set up as a user.

You will receive an email invitation with a link to the Priority Software online scheduling site. This link includes instructions for setting your password and logging in to the scheduler. 

You will have web access to the BBRB Flow Cytometry Facility or the Shelby Flow Cytometry Facility. Your login email address is:  blazerid@uab.edu

Questions:

Contact Blake Glover by email.

Contact the facilities for questions related to capabilities, training, equipment or scheduling.

Flow Cytometry Web Scheduling

Equipment


accuri overview The BD Accuri C6 Cytometer system is equipped with a blue and red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of FITC, PE, PerCP, and APC. A compact optical design, fixed alignment, and pre-optimized detector settings make the system easier to use. 

Location: BBRB 557

srv-hypercytautosampler
The HyperCytautosampler allows for high throughput flow cytometric analysis. Samples from 96- or 384 well plates are analyzed in a time-resolved manner. The HyperCytautosampler is currently connected to a FACSCalibur, which allows for 3 color analysis.

HTS flow cytometry ideally suited for:
  • Large sample sets from patient studies
  • Hybridoma identification
  • Drug screening
Location: BBRB 557
 
srv-facsaria The FACSAria is a state of the art cell 4-laser sorter; 4-way sorting of up to 14 parameters is possible. The two machines have different filter and lasers set-ups.
Location: BBRB 557 and SHEL 271
srv-bioplex
The BioPlex suspension array system offers protein and nucleic acid researchers a reliable multiplex assay solution that permits analysis of up to 30biomolecules in a single sample.
BBRB 557
srv-bdlsr2 The LSR II is a state of the art 4-laser flow cytometer. Analysis can be up to 18 parameters; quantum-dot enabled.
Location: BBRB 557 and SHEL 271*
* An HTS system is available in SHEL 271
 
srv-facscalibur

FACSCalibur is a 2-laser flow cytometer that can detect up to four colors such as FITC, PE, PE-Cy5, or APC. A carousel system is available for automatic sample collection.
Location: SHEL 271
srv-facscan

FACScan is a user-friendly flow cytometer that can detect up to three colors such, as FITC, PE, and PerCP or PE-Cy5.
Location: SHEL 271
srv-automacs The autoMACS™ Separator is a benchtop instrument for high-speed automated cell sorting (Miltenyi) using magnetic beads. Positive and negative selection possible.
Location: SHEL 271


Fees


All Assisted Services Fees: $75 / hour

Self-Service Equipment Costs:
LSR II: $50 / hour
FACScan: $35 / hour
FACSCalibur: $35 / hour
BioPlex analysis: $50 / analyzed plate
HTS fees are negotiated on a per-project basis

Training Fees: Not all training has associated costs. Costs depend on the proficiency of the user and the type of equipment.

Contact the facility directly for more information or to schedule training for specific equipment.

  Flow Cytometry Web Scheduling

Useful Links


A 12-minute video introduction to flow cytometry: A must-see for beginners :
http://probes.invitrogen.com/resources/education/tutorials/4Intro_Flow/player.html 

Flow Cytometry – A Basic Introduction by Michael G. Ormerod
http://flowbook.denovosoftware.com/ 

Practical Flow Cytometry --- Howard Shapiro
http://www.beckmancoulter.com/wsrportal/wsr/research-and-discovery/products-and-services/flow-cytometry/practical-flow-cytometry/index.htm 

A useful Java applet put together by Joe Trotter of BD Biosciences. You can view the excitation and emission spectra of many common fluorescent dyes for help in panel design.
http://www.bdbiosciences.com/research/multicolor/tools/index.jsp 

An applet similar to the above that has all of the Invitrogen reagents, as well as common fluorochromes
http://www.invitrogen.com/site/us/en/home/support/Research-Tools/Fluorescence-SpectraViewer.html

For FlowJo users: http://flowjo.typepad.com/ 

Excellent information on flow cytometry applications, especially intracellular staining
http://maeckerlab.typepad.com/maeckerlab_weblog/maeckerlab_protocols_and_tools/ 

Invitrogen: The current home of the legendary Molecular Probes materials and lots of other useful information:
http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-and-Tissue-Analysis/Flow-Cytometry.html 

Cell Signaling
http://www.cellsignal.com/technologies/flow.html 

Millipore GUAVA
http://www.millipore.com/flowcytometry/fc/fchome&open&cid=U0021 

Intellicyt --- HyperCyt autosampler for high throughput flow cytometry (available in CFAR Core)
http://www.intellicyt.com/instruments/hypercyt-sampler

Contact


BBRB Location
Director: Olaf Kutsch, PhD
Manager: Marion Spell, BS
E-mail: mlspell@uab.edu
Phone: (205) 975-3227

Bevill Biomedical Research Building
Room 557
845 19th Street South
Birmingham, Alabama, 35294
Shelby Location
Director: John D. Mountz, MD, PhD
Manager: 

E-mail: 
(205) 934-1362

Shelby Biomedical Research Building
Room 271
1825 University Blvd
Birmingham, Alabama, 35294