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Improvements in motor function and memory suggest human neurodevelopmental disorders may be amenable to treatment, even after onset of symptoms. According to a researcher, neurodevelopmental disorders with intellectual disability and autism may not need to last a lifetime.

lucas pozzo miller 2017Lucas Pozzo-Miller, Ph.D.After learning that a small-molecule drug improves breathing in a mouse model of the neurodevelopmental disorder Rett syndrome, University of Alabama at Birmingham researcher Lucas Pozzo-Miller, Ph.D., wondered if he could test it on other brain functions.

Pozzo-Miller has now found that the brain penetrant drug — a small-molecule mimetic of BDNF, or brain derived neurotrophic factor — is able to improve brain performance in Rett syndrome mice — specifically synaptic plasticity in the hippocampus and object location memory. The hippocampus is involved in learning and memory.

This finding, in collaboration with Frank Longo, M.D., of Stanford University, who had shown the drug’s improvement of breathing deficits in Rett mice in collaboration with David Katz, Ph.D., of Case Western Reserve University, adds to the growing realization that neurodevelopmental disorders that affect early brain development may be amenable to treatment, even after the onset of symptoms, says Pozzo-Miller, a professor of neurobiology in the UAB School of Medicine.

“Neurodevelopmental disorders with intellectual disability and autism may not need to last a lifetime,” Pozzo-Miller said. This offers hope to many patients and their families and caregivers.

In mouse experiments by Longo collaborating with other laboratories around the country, the drug LM22A-4 has also been shown to promote motor recovery after hypoxic-ischemic strokes, improve motor impairment in Huntington’s disease and enhance recovery of limb function after spinal cord injury in mice.

Rett syndrome affects about one of every 10,000 females worldwide. Infants develop typically until 6-18 months of age, when symptoms of intellectual disability, autistic features, deficits in motor control and sensory perception, breathing irregularities, and epilepsy start to appear. Most Rett syndrome individuals have a loss-of-function mutation in the gene for a transcriptional regulator, MeCP2.

This mutation reduces BDNF in the brains of Rett syndrome individuals and the brains of Rett-model mice. LM22A-4 is a mimetic of the BDNF loop domain, and it is a partial agonist of the BDNF receptor TrkB.

“Neurodevelopmental disorders with intellectual disability and autism may not need to last a lifetime.”

Pozzo-Miller, Longo and colleagues found that a four-week systemic treatment of female mice that have one mutant MeCP2 gene improved their ability to note that an object had been moved in the hippocampal-dependent, object location memory test and restored long-term potentiation in the hippocampus — a phenomenon underlying the plasticity of brain synapses. It also increased the distance mice traveled in an open field test, a measure of general locomotor activity, to normal levels.

The researchers dug deep into brain neurobiology to show that LM22A-4 improves spatial memory by subduing excitatory synaptic transmission and network activity in the hippocampus to levels that allow induction of synaptic plasticity and behavioral learning and memory.

Co-authors of the paper, “A small-molecule TrkB ligand restores hippocampal synaptic plasticity and object location memory in Rett syndrome mice,” published in Disease Models & Mechanisms, are Wei Li, Alba Bellot-Saez and Mary L. Phillips, UAB Department of Neurobiology and the UAB School of Medicine Civitan International Research Center; and Tao Yang, Stanford University School of Medicine Department of Neurology and Neurological Sciences.

Funding came from the Rett Syndrome Research Trust, Rettsyndrome.org, and National Institutes of Health grants NS-065027 and HD-074418.

Proposal Deadline:                        Wednesday, August 30, 2017
Pilot Grant Start Date:                   October 1, 2017
Guidelines - Subject Line:            Next-generation Proposal “Last Name”
Submit to:                                      Vicki Hixon  -   Email: vhixon@uab.edu

The Civitan International Research Center (CIRC) is requesting proposals for pilot research projects requiring whole-genome sequencing technologies to enhance our understanding of typical and atypical brain development, autism spectrum disorders, intellectual disabilities (e.g. Down, Fragile X, Rett syndromes), developmental disabilities, impaired cognitive development, and the effects of environmental toxins on the development of the human brain.

Eligible applicants include UAB faculty without NIH, NSF, or DoD support for genome-wide sequencing approaches. Investigators who need to introduce genome sequencing or related applications (RNA-seq, ChIP-seq, bisulfite sequencing, ATAC-seq, and single-cell sequencing) in their current research programs are strongly encouraged to apply. Applicants may request a budget of up to $10,000 in sequencing-related services, for use at the HudsonAlpha Genome Services Lab or the UAB Heflin Genomics Core. The award will be for 1 year with the option for a 2nd year based upon progress.

Proposals should consist of a single PDF document containing: 1) a 2-page description of the research project, with a detailed description of the experimental design and sequencing needs, and 2) the applicant’s CV with a complete list of current and pending funding.

The mission of the Civitan International Research Center is to improve the well-being and the quality of life of individuals and families affected by neurodevelopmental disabilities; to provide interdisciplinary clinical and research training in neurodevelopmental disabilities; to utilize this knowledge to develop and provide high quality exemplary services and programs; and to exchange information in a timely way with consumers, practitioners, scientists, and society.
In appreciation of donating his time and talents to the Civitan International Research Center, Ken Jackson was honored on June 25, 2017 at the Centennial Convention held in Birmingham, Alabama. For years, Mr. Jackson has provided his majestic voice as a narrator to the video as he highlighted the research being conducted at the Research Center.  
Ken jackson 20174
Alan Percy, MD, Interim Director Civitan International Research Center
Ken Jackson, Honoree
Duane Capps, Civitan International President