Irwin 2
Karleigh Irwin, an incoming junior high-school student in the Alabama School of Math and Science in Mobile, has done her summer internship in the Parpura laboratory under the direct guidance of Vladimir Grubišić, a graduate student in Neurobiology program. Karleigh has investigated the morphological appearance of astrocytes based on their dual expression of two genetically encoded and spectrally compatible
fluorescent probes, one that is membrane bound (green; Lck1-26-EGFP) and the other that assumes the cytoplasmic location (red; mRFP) (see overlay picture).   In order to achieve this goal, Karleigh has learned how to amplify cDNA, transfect primary astrocytes in culture and image live cells using fluorescence microscopy. In addition to this, Karleigh also monitored the intracellular calcium dynamics in astrocytes and C2C12 cells using the genetically encoded calcium indicator, R-Camp1h. Karleigh presented her results in the talk entitled “Morphology of astrocytes, calcium buffering” on July 24, 2013. The work and talk were very well received by the audience, consisting of the Parpura  research group and guests.