State-of-the-art bioenergetics, oxidative stress analysis and metabolite analysis support for AKI research

Comprehensive resource that includes optimized protocols and technology for

• bioanalytical analysis of oxidative stress and cellular bioenergetics
• biomarkers of AKI
• post-translational modifications
• changes in small molecule biochemistry

Consultation, training and experimental design

Recovery of adequate samples that are suitable for analysis and assay performance

Techniques in

• Mitochondrial bioenergetics
• LC-mass spectrometry based assays for creatinine, F2-isoprostanes, oxidized fatty acids and citric acid cycle intermediates
  • Sample Requirements for Creatinine Assay
  • Sample Preparation for oxidized fatty acid LC-MS
• nano LC-MS for targeted and untargeted metabolomics for clinical and animal model sample.