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Please cite grant numbers: NIH P30 AR048311 and NIH P30 AI27667
In 1986, the UAB Department of Medicine obtained institutional funds for the purchase of a FACStar four-parameter flow cytometer and cell sorter with a single laser. This instrument served as the basis for the CFCC at Shelby, which was established as a core within the UAB CAMAC in 1987. In 1988, an NIH Small Instrumentation Grant (S15CA46213), sponsored by the Dean of the School of Dentistry at UAB, provided partial funding for the purchase of a FACScan. The success of the CFCC at Shelby in rapidly integrating this technology into the research base is indicated by the support of seven different departments and divisions within the Schools of Medicine and Dentistry for the acquisition of a FACStarplus six parameter, dual laser cytometer/sorter in 1989. After a needs assessment in 1996, a successful NIH/NCRR Shared Instrumentation Grant (S10 RR11365) was submitted, and with a subsequent competitive, internal grant from the UAB Health Services Foundation General Endowment Fund, the CFCC at Shelby purchased new equipment in 1997, including a FACS Calibur, a FACS Vantage and two independent workstations. In September 2006 a successful NIH/NCRR Shared Instrumentation Grant (S10RR022468) led to the acquisition and installation of a BD Biosciences LSR II four laser, 20 detector analytic flow cytometer.
In August 2010, the CFCC at Shelby has officially established a partnership with the UAB Comprehensive Flow Cytometry Core at BBRB supported by the Center for AIDS Research (CFAR) through a consolidation grant supported by an ARRA award (3P30AI027767-22S1). This also lead to the purchase of a new 4-laser/14 parameter FACSAria II sorter for the CFCC at Shelby and an upgrade of the existing CFAR FACSAria sorter. This consolidated CFCC intends to reduce operational costs, extend services and hours offered, improve quality of services, and adapt the UAB flow cytometry core facilities to meet future challenges.
In 2014, the Core obtained support through the UAB Health Services Foundation General Endowment Fund to upgrade the LSRII analyser into a 5 laser/20 fluorochrome cytometer and the ARIAII into a 4 laser/16 fluorochrome sorter. In the same year, the Core collaborated with the VA Birmingham Medical Center and acquired a Fluidigm C1 single cell sorter and a BioMark™ HD gene expression analysis system to facilitate single cell genetic and genomic analyses capability at CFCC. To streamline flow cytometry analytical function, the Core further obtained funding through the UAB Health Services Foundation General Endowment Fund to obtain an Autosampler equipped 4 laser/15 fluorochrome Attune NxT acoustic-assisted hydrodynamic focusing flow cytometer.
The CFCC at Shelby is currently supported by the NIH P30 Rheumatic Diseases Core Center grant (P30 AR-048311), Birmingham VA Medical Center, UAB HSF-GEF, University-wide Interdisciplinary Research Center (UWIRC) program, and UAB Center for Clinical and Translational Science (CCTS).
Starting in 2000, the Core was successfully managed by a very dedicated Manager, Ms. Enid Keyser. Ms. Keyser has officially retired on Aug 31st, 2016. The CFCC at Shelby is currently operated by a new Manager, Mr. Vidya Sagar Hanumanthu (or Sagar). Dr. Shanrun Liu has been recruited to facilitate the single cell analysis component of the Core.