Please cite grant numbers:  NIH P30 AR048311 and NIH P30 AI27667

Click here


 

 Dr. John Mountz, Director of the CFCC at Shelby, discusses the equipment available in a video that can be accessed by clicking here.

 Instrument

 Year Acquired

Source

Type Excitation Wavelength (nm)

 No. of Fluorescence Detectors

FACSAria II 2010 BD BioSciences Sorter 405, 488, 561, 640 16
LSRII Analyzer  2006 BD BioSciences Analyzer  350, 405, 488, 532, 635 20
BD FACSCalibur Analyzer   BD BioSciences

Analyzer

488, 635

4

Attune Nxt 2016 Thermo Fisher

Analyzer

405, 488
561, 640
15
AutoMACS 2006 Miltenyi MACS Sorter Not Applicable Not Applicable

 

  • For flow cytometry panel design, the Core has uploaded the laser and configuration of each equipment to the FluoroFinder website (https://fluorofinder.com/). We encourage users to utilize this website to help them design their flow cytometry staining panels. Once you have registered at this website, you will find “CAMAC Flow Cytometry Core – Shelby Location” listed under the “University of Alabama of Birmingham (UAB)”. Once you select the Core, you can select the equipment under the “Choose a Cytometer” menu. You are then ready to design your panel.
  • For flow cytometry service fees, please click here.

 


 

Below are the specifications of the instruments available at the Wallace Tumor Institute (WTI), room 502

 

 Instrument

Type

Excitation Wavelength (nm)
Number of Fluorescence Detectors

Analysis Software

BD FacsSorter Analyzer 488nm
633nm
4 CellQuest
XP5FacScan Analyzer 488 nm
633 nm

5

FL1: FITC, GFP, CFSE
FL-2: PE, PI
FL-3: PerCP, PE-Cy5, PE-Cy5.5
FL-4: APC
FL-5: APC-Cy7

FlowJo Collector's Edition Acquisition Software