Jianzhong Chen, PhD

Jianzhong Chen, PhD

Assistant Professor
University of Alabama at Birmingham
Department of Vision Sciences
525 Henry Peters Building
1716 University Boulevard
Tel: (205) 934-8230
E-mail: jzchen@uab.edu
Website


Education

Postdoctoral – University of Michigan-Ann Arbor, – Washington State University
Ph.D. – University of Nebraska-Lincoln
B.S. – University of Science & Technology of China

Research/Clinical Interest

  1. Establish a sensitive, high throughput and reliable approach for analysis of lipid, protein and metabolite components of human tear film

    I am interested in developing a sensitive approach for analyzing meibum (i.e., meibomian gland secretions) and tears collected in microcapillaries, a collection method with negligible contamination from other sources. Particularly, for protein analysis, I will utilize MS and MS/MS analysis of intact proteins (i.e., the top-down approach) for more accurate detection of protein isoforms including proteins with truncations and different modifications (Chait BT, Mass spectrometry: Bottom-up or Top-down? Science, 2006, 314: 65-66), which will be helpful for more reliable disease diagnosis and/or treatment. After tear film components have been identified and characterized, the tear compositions of proteins and lipids will be integrated for continued study of eye diseases and thus providing a comprehensive understanding of the etiology of eye diseases.

  2. Develop a multi-stage mass spectrometry top-down approach for identification and characterization of proteins

    Top-down proteomics is a relatively new approach for identification and characterization of proteins whereby intact proteins and their fragments are directly analyzed by mass spectrometry and contrasts with traditional bottom-up proteomic approaches in which, only protein fragments resulted from enzymatic digestion are analyzed. Therefore, the top-down approach has the potential to improve the accuracy of identifying protein post-translational modifications. However, this approach is not yet mature and many challenges and pitfalls still exist that prevent this approach from being routinely used. To this end I will strive to develop a high efficiency and high throughput top-down approach, which will be applied to tear film related studies.

Recent Publications

For a detailed list of publications, please click the following links: Google Scholar  ResearchGate

  1. JZ Chen*, KB Green, KK Nichols, “Quantitative profiling of major neutral lipid classes in human meibum by direct infusion electrospray ionization mass spectrometry”, Invest. Ophthalmol. Vis. Sci., 2013, 54(8), 5730-5753.

  2. JZ Chen*, P Shiyanov, JJ Schlager, KB Green, “A pseudo MS3 approach for identification of disulfide-bonded proteins: Uncommon product ions and database search”, J. Am. Soc. Mass Spectrom., 2012, 23(2), 225-243.

  3. JZ Chen, KB Green-Church, KK Nichols*, “Author response: On the presence of (O-acyl)-ω-hydroxy fatty acids and their esters in human meibomian gland secretions”, Invest. Ophthalmol. Vis. Sci., 2011, 52(3), 1894-1895.

  4. JZ Chen, KB Green-Church, KK Nichols*, “Author response: On the presence and role of polar lipids in meibum”, Invest. Ophthalmol. Vis. Sci., 2010, 51(12), 6910-6911.

  5. JZ Chen, KB Green-Church, KK Nichols*, “Shotgun lipidomic analysis of human meibomian gland secretions with electrospray ionization tandem mass spectrometry”, Invest. Ophthalmol. Vis. Sci., 2010, 51(12), 6220-6231.

  6. JZ Chen*, P Shiyanov, LW Zhang, JJ Schlager, KB Green-Church, “Top-down characterization of a native highly intra-linked protein: concurrent cleavages of disulfide and protein backbone bonds”, Anal. Chem., 2010, 82(14), 6079-6089.

  7. JZ Chen, M Anderson, DE Misek, DM Simeone, DM Lubman*, “Characterization of apolipoprotein and apolipoprotein precursors in pancreatic cancer serum samples via two-dimensional liquid chromatography and mass spectrometry”, J. Chromatogr. A., 2007, 1162(2), 117-125.