All investigators are strongly encouraged to contact CMHAC prior to sample submission to ensure proper handling and preparation.

Mitochondrial Bioanalytical Services

  • mtDNA damage and haplotyping analysis
  • Mitochondrial Oxidative Phosphorylation Complex Activity Assays
  • Mitochondrial Protein Content Analysis (1D and/or 2D western blotting and Blue Native PAGE).
  • Mitochondrial Citrate Synthase Assay
  • Bioenergetics' Analysis of Tissues, Mitochondria, and Cells
  • Mitochondria Isolation and Preparation
  • Mitochondrial nuclear exchange (MNX) models
  • Mitochondrial isolation from tissues and cells.

Oxidative Stress Measurements

  • Ratios of Reduced to oxidized Glutathione (GSH:GSSG)
  • Determination of Oxidation of Thiols (as well as protein thiols)
  • Protein S-glutathionylation
  • F2-Isoprostane Measurements

Autophagy and Mitophagy Assessments

  • Ratio of LC3 I and II proteins by western blot.
  • Autophagic flux assays

Targeted Proteomics and Metabolomics

  • F2-Isoprostane Measurements
  • Krebs cycle and glycolysis intermediates
  • GSH:GSSG ratio measurements

Education and Training in the measures of mitochondrial health and quality control

  • Ratios of Reduced to oxidized Glutathione (GSH:GSSG)