UAB Metabolomics

4th Annual Workshop on Metabolomics

Sunday, July 17th - Thursday July 21st, 2016

Welcome to the 4th Annual Workshop on Metabolomics. The course is jointly sponsored by the National Institute of General Medical Sciences (NIGMS) as part of the NIH Common Fund Metabolomics Initiative, and the Departments of Chemistry and Pharmacology and Toxicology at UAB.


  • Overview & Agenda
  • Faculty
  • Lodging
  • Apply
  • Contact & Map
  • Acknowledgements

Overview

The themes in this fourth year of the workshop are:

  1. Design of a metabolomics experiment
  2. Sample stability and extraction methods
  3. Analytical systems (nuclear magnetic resonance and gas- and liquid chromatography-mass spectrometry)
    1. Targeted metabolomics
    2. Untargeted metabolomics
    3. Quantitative metabolomics
  4. Pre-processing of analytical data (Mzmine 2 and XCMSonline and Chenomx)
  5. Statistical analysis of the data (MetaboAnalyst, Simca, SAS)
  6. Metabolite databases (METLIN, HMDB, LIPIDMAPS, PubChem, ChemSpider)
  7. Identification of metabolites (MetaboSearch, MSMS analysis)
  8. Metabolite pathway analysis (Mummichog, KEGG, GeneGo, Ingenuity)
  9. Advanced elective sessions (Imaging mass spectrometry, isotope ratio analysis, Ion mobility, Command line and R programs)
  10. Electives will allow attendees to fine tune their training experience

Tentative Agenda - TBD


Workshop Faculty

Stephen Barnes, PhD

Dr. Barnes, Director of the Metabolomics Workshop, is Professor of Pharmacology and Toxicology and has secondary appointments in five other departments.  He started his research career in the laboratory of A. T. (Tony) James, co-inventor of gas-liquid chromatography, studying fatty metabolism in green algae and did his PhD on the carbohydrate metabolism of the acellular slime mold Physarum polycephalum at Imperial College, University of London under the tutelage of Sir Ernst Boris Chain, 1945 Nobel Laureate for the discovery of penicillin. He began his long-standing interest in bile acid metabolism at the Royal Free Hospital with Dame Sheila Sherlock and Barbara Billing. After the winter of 1975 in Alan Hofmann’s lab at the Mayo Clinic in Rochester, he took up an offer to come to the University of Alabama at Birmingham (UAB) in 1977 where he has remained ever since. In the early 1980s he applied high field NMR using superconducting magnets to unravel the proton NMR spectrum of bile acids as well as to use pulse sequences to greatly simplify the process. In 1992 he took over the development of LC-mass spectrometry in biomedical research at UAB and was the Director of the UAB Comprehensive Cancer Center Mass Spectrometry Shared Facility from 1992 to 2009. In 2009 he was appointed as the Director of the Targeted Metabolomics and Proteomics Laboratory (TMPL).  He also was the Associate Director of the Purdue-UAB Botanicals Center for Age-related Disease from 2000-2011 and the Director of the UAB Center for Nutrient-Gene Interaction from 2002-2010. These latter roles provided critical experience in experimental design, statistical analysis of –Omics data and the important role of the diet in determining the outcome of experimental models of chronic diseases.

More details about TMPL can be obtained at http://tmpl.uab.edu


Krista Casazza, PhD

Krista Casazza obtained her PhD from Florida International University in 2006, where her research focused on dietary and physical activity education interventions in adolescents. She received a postdoctoral fellowship on a T32 training grant at the University of Alabama at Birmingham (UAB) later that year under the mentorship of Drs. Jose Fernandez and Barbara Gower. During her training Dr. Casazza competed for and received an intramural pilot feasibility grant as well grant support from the Thrasher Research fund to investigate the effects of macronutrient modification (carbohydrate in particular) on reproductive hormones, insulin dynamics and body composition during a weight loss intervention in peri-pubertal African American girls. The data gathered from these studies were used in the submission of an NIH/NIDDK K99/R00 Transition to Independence Award which Dr. Casazza received in 2009. Working with the pediatric population, Dr. Casazza’s research interests have evolved into understanding the resource partitioning during critical periods of growth and development with primary focus on the bone fat interface. In addition to the R00 study, she is currently conducting an investigation of the contribution of bone marrow adipose tissue accrual to the bone-fat interface and the metabolic effects of this relationship in young children. During here four years as a postdoctoral fellow, Dr. Casazza has published 44 papers, 3 book chapters, presented numerous abstracts and invited talks at national and local meetings. She was promoted to Assistant Professor at UAB in February, 2011.

Website: http://www.uab.edu/shp/nutrition/about/faculty-and-staff/primary-faculty/25-krista-casazza


Jeevan Prasain, PhD

Dr. Prasain is an Assistant Professor of Pharmacology and Toxicology at UAB. He received his undergraduate and master’s training (Chemistry) at Tribhuvan University, Nepal and his Ph.D. at Toyama Medical & Pharmaceutical University, Japan. His expertise is in the use of NMR and LC-MS for the discovery and identification of natural products. He will be demonstrating the use of LC-MS methods for the analysis of metabolite classes.

Website: http://www.uab.edu/proteomics/massspec/personnel/prasain.php


Hemant Tiwari, PhD

Dr. Tiwari is the Co-Director of the Metabolomics Workshop. He received his Ph.D. in mathematics from the University of Notre Dame, South Bend, Indiana. While being a faculty at the University of Maine, he got interested in statistical genetics, and completed a post-doctoral fellowship in Statistical Genetics under Prof. Robert Elston in the Department of Biometry and Genetics at Louisiana State University Medical Center, New Orleans and at the Case Western Reserve University in Cleveland. Subsequently, he worked as a faculty member in the Department of Epidemiology and Biostatistics at Case Western Reserve University. In January 2002, he joined UAB as a faculty in the Department of Biostatistics (Section on Statistical Genetics). His research interests include Genetic Linkage Analysis, Disequilibrium Mapping, Population Genetics, Molecular Evolution, Bioinformatics, and Genetics of Infectious Diseases. Currently, he is involved in gene mapping studies of epilepsy, SLE, phonological disorders, and dental traits. He is the Head of the Section on Statistical Genetics, the William “Student” Sealy Gosset Professor, Director of the Biostatistics Pre-Doctoral NHLBI Training Program and Director of the Post-Doctoral NHLBI Training Program in Statistical Genetics. He also is the Director of the NIGMS-funded National Short Course in Statistical Genetics and Genomics.

Website: http://www.soph.uab.edu/ssg/people/tiwari

Lodging

Hotel options will be distributed to attendees once they have been selected to attend the workshop.

Additional Lodging Options:

Apply (2016 Workshop)

To apply, please perform the following tasks.

  1. Complete on-line application form at http://goo.gl/forms/DHLelzRnyO. Only fully completed application form will be considered.
  2. Send your current curriculum vitae to mammadu@uab.edu.
  3. Send letters of recommendation (minimum of two) to mammadu@uab.edu.
  4. Send a proposal to mammadu@uab.edu. Using a one-page proposal, describe why learning about metabolomics and its methodology would help your current research and/or influence your research career plans. If you have questions about the proposal, please contact Dr. Stephen Barnes – sbarnes@uab.edu

Attendance is limited to 40 for this course. Please apply prior to Friday, April 29, 2016. Accepted applicants will be notified by Friday, May 12th, with registration fee due by May 26th.

Women, members of under-represented minority groups, and individuals with disabilities are strongly encouraged to apply.

There will be 20 fellowships for graduate students and postdoctoral fellows attending the workshop. The fellowships will provide up to $1200 of travel and hotel expenses. Selection will be based on information provided by the applicants.

Upon Acceptance, the fee for attendees is:
$300 – for graduate students and postdoctoral fellows
$750 – for faculty at academic, government, and non-profit organizations
$1,500 – for-profit organizations

The fee includes course tuition and syllabus, breakfast, lunch and refreshment breaks and the Workshop dinner. Hotel accommodation and travel will be the attendees’ responsibility.

The fee will be payable upon acceptance into the course.

Need more information? Contact Lynn Waddell at 205 934-4579/mammadu@uab.edu

Refund and Cancellation Policy: Attendee substitutions are allowed, but notification must be made in writing by Friday, May 26th and sent to mammadu@uab.edu or by fax at 205.934.8240. After this date no substitutions will be granted. UAB reserves the right to cancel this conference, in which case a full refund of your registration fee will be provided. We are unable to refund any travel costs (flight, hotel, etc.) in the case of UAB’s cancellation.

Recording and Photography Clause: UAB reserves exclusive rights to record (audio and video) and/or photograph all conference proceedings for use in marketing materials, presentations and course content sales.

The fee will be payable upon acceptance into the course.

Need more information?  Contact Lynn Waddell at 205 934-4579/mammadu@uab.edu

For enquiries please contact

Stephen Barnes
205-934-7117
sbarnes@uab.edu
Lynn Waddell
205-934-4579
mammadu@uab.edu
 

Map

Volker Hall - 1670 University Blvd‎ (UAB Campus Map)


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Chemistry Building - 901 14th Street South (UAB Campus Map)


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McCallum Basic Health Sciences Bldg - 1918 University Blvd (UAB Campus Map)


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LRC - 1714 9th Avenue South (UAB Campus Map)



Bevill Biomedical Ssciences - 845 19th Street South (UAB Campus Map)

Acknowledgements

Many thanks to

  1. NIH Common Fund R25 GM103798-03
  2. Mary-Ann Bjornsti, PhD (Chair, Pharmacology & Toxicology)
  3. Richard A Dluhy, PhD (Chair Department of Chemistry)
  4. UAB School of Medicine
  5. Comprehensive Cancer Center
  6. Office of the UAB Vice-President for Research
  7. UAB-UCSD O’Brien Acute Kidney Injury Center
  8. Diabetes Research Center & Comprehensive Diabetes Center
  9. Center for Free Radical Biology
  10. Agilent, Sciex and Waters