barnesProfessor

Areas of Interest
Bile acids, polyphenols, metabolomics, NMR, mass spectrometry

PubMed

Biography

Dr. Barnes was trained in Chemistry and Biochemistry in the UK. He joined UAB in 1977 and pursued research on bile acids and latterly polyphenols, particularly soy isoflavones. He was promoted to Associate Professor (with tenure) in 1985 and Full Professor in 1994. He has received NIH support from several areas to pursue the conjugation of bile acids, the chemistry, biochemistry and physiology of polyphenols, the underlying chemistry of lens cataracts and the use of omics approaches in biomedical research. He was the Associate Director of the Purdue UAB Botanicals Center for Age Related Disease and the UAB Center for Nutrient Gene Interaction. He was an elected member of the National Advisory Council for the Center for Complementary and Alternate Medicine and the NIH Council of Councils. He is currently the Director of the NIH funded Workshop in Metabolomics. He received the 2012 Distinguished Faculty Award.

  • Research Interests

    I have had a longstanding interest in metabolism and enzymology and the enterohepatic circulation of endogenous and exogenous metabolites. Because of early training in hepatobiliary diseases, this led to studies on bile acid metabolism and purification of the enzymes catalyzing their formation. A second strong area of research has been the roles of polyphenols in health and disease, with a particular emphasis on soy isoflavones. Over the past decade, I have been pursuing the basis of lens cataract formation in the lens. An overarching theme for these research areas has been the pathways of metabolism and the utilization of mass spectrometry for their study. This had led to a strong interest in metabolomics and training in this re-emerging discipline.

  • Selected Publications

    Sweeny, D.J., S. Barnes, G.D. Heggie, R.B. Diasio.  Metabolism of 5-fluorouracil to an N-cholyl-2 fluoro beta alanine conjugate: Previously unrecognized role for bile acids in drug conjugation.  PNAS 84: 5439-5443, 1987.

    Johnson, M., S. Barnes, J. Kwakye, R.B. Diasio.  Purification of human liver cholyl CoA:amino acid N-acyltransferase.  J Biol Chem 266: 10227-10233, 1991

    Falany, C.N., Xie, X, Wheeler, J.B., Wang, J., Smith, M., He, D., Barnes, S.  Molecular cloning and expression of rat liver bile acid CoA ligase. J Lipid Res, 43: 2062-2071, 2002.

    Barnes, S., J.M. Geckle.  High resolution nuclear magnetic resonance spectroscopy of bile salts:  individual proton assignments for aqueous sodium cholate at 400 MHz.  J Lipid Res 23: 161-170, 1982.

    Waterhous, D. V., S. Barnes, D. D. Muccio.  Nuclear Magnetic Resonance Spectroscopy of Bile Acids.  Development of two dimensional NMR methods for the elucidation of proton resonance assignments for five common bile acids, and their parent bile acid, 5 beta-cholanoic acid.  J Lipid Res 26: 1068-1078, 1985

    Isom, A.I., Wilson, L., Kirk, M., Coward, L., DarleyUsmar, V.M. and Barnes, S. Modification of cytochrome c by 4 hydroxy-2 nonenal: evidence for histidine, lysine and arginine aldehyde adducts. J Amer Soc Mass Spectrom 15: 1136-1147, 2004

    McClure M, DeLucas LJ, Wilson L, Ray M, Rowe S, Wu X, Dai Q, Hong JS, Sorscher EJ, Kappes JC, Barnes S. Palmitoylation and other posttranslational modifications of CFTR with relevance to gating and processing. Protein Eng Des Sel. 2012 Jan;25(1):7-14. PMCID: PMC3276306

    Stella DR, Floyd KA, Grey AC, Renfrow MB, Schey KL, Barnes S. Tissue localization and solubilities of αA-crystallin and its numerous C-terminal truncation products in pre- and postcataractous ICR/f rat lenses. IOVS. 2010;51:5153-61. PMCID: PMC3066599.

    Barnes S, et al. Training in metabolomics research. I. Designing the experiment, collecting and extracting samples and generating metabolomics data.  Journal of Mass Spectrometry, 2016 July;51:461-475. 

    Barnes S, et al. Training in metabolomics research. II. Processing and statistical analysis of metabolomics data, metabolite identification, pathway analysis, applications of metabolomics and its future. Journal of Mass Spectrometry, 2016 August 4;51:535-548. 

Education

Ph.D., University of London (Imperial College), UK

Postdoctoral Fellowships
Royal Free Hospital, London, UK
Informatics, UAB

Contact

Office Location
BBRB 711

Phone
205-934-7117

Email
sbarnes@uab.edu