This 680 square foot facility utilizes the following integrated platform of systems for HTP nano-crystallization for aqueous and membrane proteins as well as the co-crystallization of drug protein complexes with a capacity to perform thousands of experiments a day in nano to microliter volumes. The facility also has several other novel techniques to optimize crystal growth in order to obtain the diffraction quality crystals necessary for structure determination.     

Equipment includes:

  • A Rigaku Phoenix RE Liquid Handling System for high throughput, low volume protein crystallization experimentation – the Rigaku Phoenix
  • An automated HTP liquid dispensing systems for submicroliter volumes - the Cartesian HoneyBee™ System
  • An automated microchip system for free interface diffusion crystallization along with an automated imaging station for microchips - the Fluidigm Topaz Microchip System™ 
  • An automated HTP capillary counter diffusion system - the HoneyComb by Genomic Solutions™
  • High-throughput lipidic (in cubo) protein nano-crystallization system for membrane proteins
  • A HTP automated system for optical recognition and scoring of crystals - Crystal Score™
  • Formulatrix Imaging Systems with SONICC
Use of a virtual screening program (utilizing predictive algorithms) for the optimization of crystallization conditions, thus reducing amount of sample and number of experiments needed to define high quality crystal growth.  This technology supports efforts to find new or optimize present conditions that improve crystal growth for both soluble and membrane proteins.