With the increasingly widespread use of digital media to capture scientific images and data, the possibilities for making alterations to images has expanded enormously. This module is about deciding which alterations are acceptable and which are not. The presentation is a lecture by Douglas Cromey, a microscopist who has been involved nationally in setting standards. The lecture is about 45 minutes and includes PowerPoint slides. The video and written case studies can be done online or used in the classroom or lab meeting. In the Useful Links section are image manipulation guides and a video demonstration of them in Photoshop, further video cases, and more.