Services provided

Aim 1: To characterize mouse models of HRFD in vivo.

Aim 2: To establish mTERT and hTERT immortalized cell lines

Aim 3: Physiological and molecular characterization of HFRD cell lines.

Currently Available Resources

Characterize Mouse and Rat models of ARPKD

  • Telemetry blood pressure
  • Chronic GFR measurements
  • MRI of liver and kidney cystic progression
  • Immunofluorescence\immunohistochemistry\histology services
  • In vivo multiphoton confocal imaging of cell pathways including calcium and cAMP using biosensors

Generate Cell lines from kidney and liver

  • Human
  • Mouse
  • Rat

Characterization of HRFD Cells lines

  • Cell pathway analysis using a variety of approaches over time
  • Renal tubular microperfusion and patch clamp
  • RNA seq analysis
  • Proteomic analysis
  • Genetic and proteomic consultation

Request Form