A1R Confocal

Features:

  • Nikon TE2000 inverted microscope
  • Prior Scientific motorized XY stage
  • Multiple region time lapse with z-stack for 3D imaging. Timelapse and Spectral detection can be done on the A1 as well as Fluorescence Recovery After Photobleaching (FRAP) experiments. With the addition of a resonance scanner the A1 can image frames at over 300fps!  It can easily be switched from standard galvo scanning to Resonance or Spectral mode within the Nis Elements software.
  • Nikon Perfect Focus
  • Photo-manipulation (photo-bleaching/photo-activation) experiments can be easily conducted with multiple user-defined regions of interest
  • Tandem galvano and resonant scanner up to 30 512X512 images/sec
  • Advanced Detection: 4 PMT detectors and a transmitted light detector The detection system in the A1 Scanner consists of a highly sensitive array of PMT's which are capable of integrating line scans at a faster rate than previous confocals were able to accomplish making the system ideal for imaging large amounts of data.

Objectives:

  • Plan Apo λ 4x dry
  • Plan Fluor 10x DIC L N1 dry 20X 0.75 NA
  • Plan Apo VC 20x DIC N2 dry
  • Plan Fluor 40x Oil DIC H N2
  • Plan Apo 60x Oil λS DIC N2
  • Plan Fluor 100X 1.49 NA, Oil, DIC

Laser Lines(nm):

404, 457, 476, 488, 514, 568, and 638

Filter Cubes(eyes):

DAPI, FITC, TRITC, CFP, YFP, RGB

Accessories:

  • Tokai Hit incubation stage chamber at 37ºC, optional 5% CO2
  • Motorized x-y stage
  • Z-stacks
  • Nikon Perfect Focus System

Acquisition Software:

The Nis Elements 5.0 Imaging Software contains a cutting-edge analysis package of modules for doing quantification and densitometry of fluorescence in 2D and 3D.  Nis Elements can also be used to count cells and track cells on time-lapse movies.



A1R Confocal Image 2 no text
The HRIF houses three High-Resolution confocal microscopes that can image live and fixed cells and tissue. 


BPAE Cells 60x zoom


Polen3 Deconvolved TypeBlind Cross section Movie gif 001