Overview

The Structural Biology Program supports UAB faculty, post-graduate, graduate and undergraduate students with cutting-edge technology to elucidate structure-function relationships of macromolecules. The SBP receives generous support for these technologies through the UAB School of Medicine and UAB Comprehensive Cancer Center.

UAB Shared Resources

technologyProviding the following technologies for elucidating 3D structural information:

  • High throughput robotic Nano-Crystallization and Imaging (X-ray)
  • State-of-the-art in house X-Ray Diffraction equipment for 3D crystal structure determination (X-ray)
  • Dedicated access to the synchrotron radiation facility through SERCAT beamline, Advanced Photon Source, Argonne National Laboratory (X-ray)
    • Cryo-Electron Microscopy
    • Nuclear Magnetic Resonance
    • Structural Mass Spectrometry (e.g. hydrogen deuterium exchange, ion mobility)

Additional Biophysical Technologies

  • Differential Scanning Calorimetry (DSC)
    • Evaluate the thermodynamics of thermal unfolding
  • Isothermal Titration Calorimetry (ITC)
    • Derive equilibrium constants and stoichiometry
  • Differential scanning fluorimetry (nano-DSF)
    • Evaluate protein stability and aggregation
  • Dynamic Light Scattering
    • Evaluate protein aggregation (second virial coefficient)
  • Biacore (SPR)
    • Perform kinetic analysis of macromolecular interactions
  • Circular Dichroism
    • Assessment of secondary structural elements