Recent publications from TMPL

Increased flux through the mevalonate pathway mediates fibrotic repair without injury.
Larson-Casey JL, Vaid M, Gu L, He C, Cai GQ, Ding Q, Davis D, Berryhill TF, Wilson LS, Barnes S, Neighbors JD, Hohl RJ, Zimmerman KA, Yoder BK, Longhini ALF, Hanumanthu VS, Surolia R, Antony VB, Carter AB.
J Clin Invest. 2019 Oct 14. pii: 127959. [doi: 10.1172/JCI127959] [Epub ahead of print] [PMID: 31609245]

Quantitative Proteomics Uncovers the Interaction between a Virulence Factor and Mutanobactin Synthetases in Streptococcus mutans.
Rainey K, Wilson L, Barnes S, Wu H.
mSphere. 2019 Sep 25;4(5). pii: e00429-19. [doi: 10.1128/mSphere.00429-19] PMID: 31554721

Relative stabilities of wild type and mutant glial fibrillary acidic protein in patients with Alexander disease.
Heaven MR, Wilson L, Barnes S, Brenner M.
J Biol Chem. 2019 Sep 4. pii: jbc.RA119.009777. [doi: 10.1074/jbc.RA119.009777] [Epub ahead of print]

More Publications

Annual Workshop on Metabolomics

Annual Workshops on Metabolomics have been held from 2013-2018 on the campus of the University of Alabama at Birmingham, jointly sponsored by the National Institute of General Medical Sciences (NIGMS) as part of the NIH Common Fund Metabolomics Initiative, and the Departments of Chemistry and Pharmacology and Toxicology at UAB.

Materials (PDFs and videos) presented at these workshops can be found on the Metabolomics Workshop web page.

Due to the need to move the Targeted Metabolomics and Proteomics Laboratory to a new building during the summer of 2019, the summer metabolomics workshop will not be held this year. Future workshops will be refocused to give a full hands-on experience in metabolomics. Those interested in participating in these workshops should contact Dr. Stephen Barnes (

About TMPL

The Targeted Metabolomics and Proteomics Laboratory (TMPL) is organized to provide a variety of analytical and technical services using mass spectrometry to UAB investigators. The laboratory has three mass spectrometers, a triple quadrupole instrument (SCIEX 4000), a quadrupole-linear ion trap instrument (SCIEX 6500 Qtrap), and quadrupole-TOF (SCIEX 5600 TripleTOF). The SCIEX 5600 TripleTOF is particularly powerful for comprehensive and targeted proteomics, lipidomics and metabolomics. The combination of Eksigent microflow 200 LC and 415 nanoLC with the mass spectrometers provides the highest possible sensitivity for sample analysis. The SCIEX 6500 Qtrap is fitted with a SelexION™ interface for ion mobility studies.

Contact Dr. Matt Renfrow about use of hi-resolution Orbitrap Velos instrument (; 205 996-4681).

A Bruker MALDI TOF-TOF instrument is available in the Comprehensive Cancer Center Core Laboratory of Dr. Jim Mobley (; 205 996-6363).

New sample and data-handling arrangements in TMPL – investigators please note

Figure from J Chromatogr B Analyt
Technol Biomed Life Sci.
[ PMID: 20346741]