Chromatography

Liquid chromatography tandem mass spectrometry (LC-MS) was carried out with a Shimadzu Prominence HPLC system and SCIEX 4000 mass spectrometer. An aliquot of 10 µl of each sample was injected onto an Normal Phase Intrada Amino Acid column 50 × 3 mm; 3 μm at 35^oC for gradient separation. Mobile phases were: A) ddH₂O with 100 mM ammonium formate; and B) Acetonitrile:water:formic acid (v:v:v = 95:5:0.3). Gradient schedule is the following: 0–3.0 min, 92%–88% Solution B; 3.0–6.4 min, 88%–70% Solution B; 6.4–6.5 min, 70%–0% Solution B; 6.5–10 min, 0% Solution B; 10–10.1 min, 0–92% Solution B; 10.1–13 min, 92% Solution B, with a total run time of 13 min. The flow rate is 600µL/min.

Extraction Protocols:

Extraction protocols for sample processing are dependent on the matrices involved. Please see extraction methods section for more specific instructions.

References

Method derived from the following

[1] Liu, Z., Tu, M.-J., Zhang, C., Jilek, J. L., Zhang, Q.-Y., & Yu, A.-M. (2019).
A reliable LC-MS/MS method for the quantification of natural amino acids in mouse plasma: Method validation and application to a study on amino acid dynamics during hepatocellular carcinoma progression.
Journal of Chromatography B, 1124, 72-81. doi:10.1016/j.jchromb.2019.05.039

Resources

This tab might be populated with additional information like PowerPoint slides from presentations at conferences, class presentations, grant associated links, and other components that would be directly tied to the method.