Prostaglandin MRM Method

Measured Analytes

  1. 6-keto PGF1 alpha
  2. 8-iso-PGF2 alpha
  3. PGF2 alpha
  4. PGE2
  5. PGD2


Liquid chromatography tandem mass spectrometry (LC-MS) was carried out with a Shimadzu Prominence HPLC system and SCIEX 6500 mass spectrometer. An aliquot of 20 µl of each sample was injected onto an Synergi 3 µm C18 250 x 2.1 mm column at 50oC for gradient separation. Mobile phases were: A, ddH2O with 0.1% Formic Acid; and B, Acetonitrile with 0.1% Formic Acid. Gradient schedule starts at 10% B and is linearly increased to 80% B at 11 minutes, then 100% B at 14 minutes, and then returned to 10% B at 16 at minutes and re-equilibration until 20 minutes. Time per sample injection was 20 minutes.

Extraction Protocols:

Extraction protocols for sample processing are dependent on the matrices involved. Please see extraction methods section for more specific instructions.


Method derived from the following

[1] PLoS Genet. 2013 Jan; 9(1): e1003271.
Published online 2013 Jan 31. doi: 10.1371/journal.pgen.1003271
A Heterogeneous Mixture of F-Series Prostaglandins Promotes Sperm Guidance in the Caenorhabditis elegans Reproductive Tract
Hieu D. Hoang,  Jeevan K. Prasain,  Dixon Dorand,  and Michael A. Miller, *


This tab might be populated with additional information like PowerPoint slides from presentations at conferences, class presentations, grant associated links, and other components that would be directly tied to the method.