Tryptophan Metabolites MRM Method

Measured Analytes

  1. Nicotinic Acid
  2. 2-Picolinic Acid
  3. Quinolinic Acid
  4. Serotonin
  5. Kynurenine
  6. 5-OH Tryptophan
  7. Tryptophan
  8. Tryptamine
  9. Indole-3-lactate
  10. Indole-3-acetate


Liquid chromatography tandem mass spectrometry (LC-MS) was carried out with a Shimadzu Prominence HPLC system and SCIEX 4000 mass spectrometer. An aliquot of 10 µl of each sample was injected onto an T3 Atlantis 3µm C18 100 x 2.0 mm column at 40oC for gradient separation. Mobile phases were: A, ddH2O with 0.1% Formic Acid; and B, Acetonitrile with 0.1% Formic Acid. Gradient schedule started at 0%B for 0.5 min and was linearly increased to 60%B at 6 minute; then rapidly increased to 95%B at 8 minutes. At 8.25 minutes the gradient was returned to starting conditions and allowed to re-equilibrate until 12.0 minutes. Flow rate was 400µL/min.

Extraction Protocols:

Extraction protocols for sample processing are dependent on the matrices involved. Please see extraction methods section for more specific instructions.


Method derived from the following

[1] A mass spectrometric method for quantification of tryptophan-derived uremic solutes in human serum
Anqi Zhang, Keshab Rijal, Seng Kah Ng, Katya Ravid, and Vipul Chitalia,
JBiol Methods. 2017; 4(3): e75.
Published online 2017 Jul 31. doi: 10.14440/jbm.2017.182
PMID: 29707597


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