Stephen Barnes, PhD

Stephen Barnes, PhD

(University of London, 1972, Biochemistry)
University of Alabama at Birmingham
Department of Pharmacology and Toxicology
BBRB 711 (UAB OneCard access required)
845 19th St S
Birmingham, AL 35205
Tel: (205) 934-7117
Fax: (205) 934-6944

Academic Appointments:

Professor of Pharmacology & Toxicology (1994-present)
Professor of Biochemistry & Molecular Genetics (1997-present)
Professor of Environmental Health Sciences (2000-present)
Professor of Genetics (2000-present)
Professor of Vision Science (2006-present)

Other appointments:

Director, Targeted Metabolomics and Proteomics Laboratory (2009-present)
Senior Scientist, Comprehensive Cancer Center
Senior Scientist, Center for Aging
Associate Director, Purdue-UAB Botanicals Center for Age-related Disease
Director, Center Nutrient-Gene Interaction in Cancer Prevention
Senior Scientist, Nephrology Research and Training Center (2008-present)
Senior Scientist, Center for Metabolic Bone Disease (2006-present)
Senior Scientist, Center for Free Radical Biology (1999-present)
Senior Scientist, Nutrition Obesity Research Center (2000-present)


2023 Publications
  1. Ekta Tiwary, Taylor F Berryhill, Landon Wilson, Stephen Barnes, Jeevan K Prasain, J Michael Wells
    LC-MS/MS method for proline-glycine-proline and acetylated proline-glycine-proline in human plasma
    J Chromatogr B Analyt Technol Biomed Life Sci. 2023 Jul 5;1228:123815. PMID: 37453387 doi: 10.1016/j.jchromb.2023.123815 Online ahead of print.
  2. Jordan SJ, Wilson L, Ren J, Gupta K, Barnes S, Geisler WM..
    Natural clearance of Chlamydia trachomatis infection is associated with distinct differences in cervicovaginal metabolites.
    J Infect Dis. 2023 May 11:jiad155. Online ahead of print.PMID: 37163744 doi: 10.1093/infdis/jiad155
  3. Livelo C, Guo Y, Abou Daya F, Rajasekaran V, Varshney S, Le HD, Barnes S, Panda S, Melkani GC.
    Time-restricted feeding promotes muscle function through purine cycle and AMPK signaling in Drosophila obesity models.
    Nat Commun. 2023 Feb 21;14(1):949. PMID: 36810287 PMCID: PMC9944249 doi: 10.1038/s41467-023-36474-4
2022 Publications
  1. Srivastava O, Wilson L, Barnes S, Srivastava K, Joseph R.
    αA and αB peptides from human cataractous lenses show antichaperone activity and enhance aggregation of lens proteins
    Mol Vis. 2022 Aug 6;28:147-164. eCollection 2022. PMID: 36540064
  2. Brothwell JA, Fortney KR, Gao H, Wilson LS, Andrews CF, Tran TM, Hu X, Batteiger TA, Barnes S, Liu Y, Spinola SM.
    Haemophilus ducreyi Infection Induces Oxidative Stress, Central Metabolic Changes, and a Mixed Pro- and Anti-inflammatory Environment in the Human Host.
    mBio. 2022 Dec 1:e0312522. [doi:10.1128/mbio.03125-22] Online ahead of print.PMID: 36453940
  3. Dianna Xing, Gloria A Benavides, Michelle S Johnson, Ran Tian, Stephen Barnes, Victor M Darley-Usmar
    Glutamine-dependent effects of nitric oxide on cancer cells subjected to hypoxia-reoxygenation
    Nitric Oxide. 2022 Nov 19;S1089-8603(22)00122-7. [doi:10.1016/j.niox.2022.11.003] PMID: 36414197
  4. Prasain JK, Wilson LS, Grubbs C, Barnes S.
    Gamma-ray Irradiation of Rodent Diets Alters the Urinary Metabolome in Rats with Chemically Induced Mammary Cancer.
    Metabolites. 2022 Oct 16;12(10):976. [doi:10.3390/metabo12100976] PMID: 36295878
  5. Carson TL, Buro AW, Miller D, Pena A, Ard JD, Lampe JW, Yi N, Lefkowitz E, William VP, Morrow C, Wilson L, Barnes S, Demark-Wahnefried W.
    Rationale and study protocol for a randomized controlled feeding study to determine the structural- and functional-level effects of diet-specific interventions on the gut microbiota of non-Hispanic black and white adults.
    Contemp Clin Trials. 2022 Oct 17:106968. [doi:10.1016/j.cct.2022.106968] Online ahead of print. PMID:36265810
  6. Chehade SB, Green GBH, Graham CD, Chakraborti A, Vashai B, Moon A, Williams MB, Vickers B, Berryhill T, Van Der Pol W, Wilson L, Powell ML, Smith DL Jr, Barnes S, Morrow C, Mukhtar MS, Kennedy GD, Bibb JA, Watts SA.
    A modified standard American diet induces physiological parameters associated with metabolic syndrome in C57BL/6J mice.
    Front Nutr. 2022 Aug 29;9:929446. [doi:10.3389/fnut.2022.929446] eCollection 2022. PMID: 36105576
  7. Mahmoudi Aliabadi P, Teuber R, Jani PK, Wilson L, Enghard P, Barnes S, Chiorazzi N, Radbruch A, Melchers F, Kubagawa H.
    Soluble Fc Receptor for IgM in Sera From Subsets of Patients With Chronic Lymphocytic Leukemia as Determined by a New Mouse Monoclonal Antibody.
    Front Immunol. 2022 Jun 16;13:863895. [doi: 10.3389/fimmu.2022.863895] eCollection 2022.PMID: 35784336
  8. Newman SD, Schnakenberg Martin AM, Raymond D, Cheng H, Wilson L, Barnes S, O'Donnell BF.
    The relationship between cannabis use and taurine: A MRS and metabolomics study.
    PLoS One. 2022 Jun 2;17(6):e0269280. [doi: 10.1371/journal.pone.0269280] eCollection 2022. PMID: 35653401
  9. Li J, Morrow C, Barnes S, Wilson LS, Womack ED, McLain A, Yarar-Fisher C.
    Gut Microbiome Composition and Serum Metabolome Profile Among Individuals With Spinal Cord Injury and Normal Glucose Tolerance or Prediabetes/Type 2 Diabetes.
    Arch Phys Med Rehabil. 2022 Apr;103(4):702-710. [doi: 10.1016/j.apmr.2021.03.043]
2021 Publications
  1. Khanal S, Bai Y, Ngo W, Nichols KK, Wilson L, Barnes S, Nichols JJ.
    Human meibum and tear film derived cholesteryl and wax esters in meibomian gland dysfunction and tear film structure.
    Ocul Surf. 2021 Nov 11;23:12-23. [doi: 10.1016/j.jtos.2021.10.009] Online ahead of print.PMID: 34774809
  2. Pak HH, Haws SA, Green CL, Koller M, Lavarias MT, Richardson NE, Yang SE, Dumas SN, Sonsalla M, Bray L, Johnson M, Barnes S, Darley-Usmar V, Zhang J, Yen CE, Denu JM, Lamming DW.
    Fasting drives the metabolic, molecular and geroprotective effects of a calorie-restricted diet in mice.
    Nature Metabolism. 2021 Oct 18. [doi: 10.1038/s42255-021-00466-9] Online ahead of print.PMID: 34663973
  3. Raines NH, Cheung MD, Wilson LS, Edberg JC, Erdmann NB, Schmaier AA, Berryhill TF, Manickas-Hill Z, Li JZ, Yu XG, Agarwal A, Barnes S, Parikh SM.
    NAD+ biosynthetic impairment and urinary metabolomic alterations observed in hospitalized adults with COVID-19-related acute kidney injury
    Kidney Int Rep. 2021 Sep 14. [doi: 10.1016/j.ekir.2021.09.001] Online ahead of print. PMID: 34541422
  4. Ziemanski JF, Wilson L, Barnes S, Nichols KK.
    Prostaglandin E2 and F Alter Expression of Select Cholesteryl Esters and Triacylglycerols Produced by Human Meibomian Gland Epithelial Cells
    Cornea. 2021 Sep 4. [doi: 10.1097/ICO.0000000000002835] Online ahead of print. PMID: 34483274.
  5. Jeevan K. Prasain, Stephen Barnes, J. Michael Wyss
    Kudzu isoflavone C-glycosides: Analysis, biological activities, and metabolism
    Food Frontiers. First published: 06 August 2021. [doi: 10.1002/fft2.105]
  6. Jia Li, Casey Morrow, Stephen Barnes, Landon Wilson, Erika D Womack, Amie McLain, Ceren Yarar-Fisher
    Gut microbiome composition and serum metabolome profile among individuals with spinal cord injury and normal glucose tolerance or prediabetes/type 2 diabetes
    Arch Phys Med Rehabil. 2021 Jun 11;S0003-9993(21)00438-X. [doi: 10.1016/j.apmr.2021.03.043] Online ahead of print.
  7. Safal Khanal, Yuqiang Bai, William Ngo, Kelly K Nichols, Landon Wilson, Stephen Barnes, Jason J Nichols
    Human Meibum and Tear Film Derived (O-Acyl)-Omega-Hydroxy Fatty Acids as Biomarkers of Tear Film Dynamics in Meibomian Gland Dysfunction and Dry Eye Disease
    Invest Ophthalmol Vis Sci. 2021 Jul 1;62(9):13. [doi: 10.1167/iovs.62.9.13] PMID: 34236383.
  8. Khanal S, Ngo W, Nichols KK, Wilson L, Barnes S, Nichols JJ.
    Human meibum and tear film derived (o-acyl)-omega-hydroxy fatty acids in meibomian gland dysfunction.
    Ocul Surf. 2021 May 27:S1542-0124(21)00048-3. [doi: 10.1016/j.jtos.2021.05.009] Online ahead of print. PMID: 34052415.
  9. Ayanabha Chakraborti, Christopher Graham, Sophie Chehade, Bijal Vashi, Alan Umfress, Pradeep Kurup, Benjamin Vickers, H. Alexander Chen, Rahul Telange, Taylor Berryhill, William Van Der Pol, Mickie Powell, Stephen Barnes, Casey Morrow, Daniel L. Smith Jr., M. Shahid Mukhtar, Stephen Watts, Gregory Kennedy and James Bibb
    High Fructose Corn Syrup-Moderate Fat Diet Potentiates Anxio-Depressive Behavior and Alters Ventral Striatal Neuronal Signaling
    Front. Neurosci., 26 May 2021. [doi: 10.3389/fnins.2021.669410]
  10. Jillian F Ziemanski, Landon Wilson, Stephen Barnes, Kelly K Nichols
    Triacylglycerol lipidome from human meibomian gland epithelial cells: Description, response to culture conditions, and perspective on function
    Exp Eye Res. 2021 Apr 10;108573. [doi: 10.1016/j.exer.2021.108573] Online ahead of print
  11. Lisa M Curtis, James George, Volker Vallon, Stephen Barnes, Victor M Darley-Usmar, Sucheta Mukul Vaingankar, Gary Cutter, Orlando Gutierrez, Michael Seifert, Joachim Ix, Ravindra Mehta, Paul W Sanders, Anupam Agarwal
    UAB-UCSD O'Brien Center for Acute Kidney Injury Research
    Am J Physiol Renal Physiol. 2021 Mar 29. [doi: 10.1152/ajprenal.00661.2020] [PMID: 33779316] Online ahead of print.
  12. Mark Messina, Sonia Blanco Mejia, Aedin Cassidy, Alison Duncan, Mindy Kurzer, Chisato Nagato, Martin Ronis, Ian Rowland, John Sievenpipe, Stephen Barnes
    Neither soyfoods nor isoflavones warrant classification as endocrine disruptors: a technical review of the observational and clinical data
    Crit Rev Food Sci Nutr. 2021 Mar 27;1-57. [doi: 10.1080/10408398.2021.1895054] [PMID: 33775173]
  13. Oak ASW, Shafi R, Elsayed M, Mishra B, Bae S, Barnes S, Kashyap MP, Slominski AT, Wilson LS, Athar M, Elmets CA
    Dietary table grape protects against UV photodamage in humans: 2. molecular biomarker studies.
    J Am Acad Dermatol. 2021 Jan 20:S0190-9622(21)00184-5. [doi: 10.1016/j.jaad.2021.01.036] Online ahead of print. [PMID: 33484768]
2020 Publications
  1. Jeevan K. Prasain, Stephen Barnes
    Cranberry polyphenols‐gut microbiota interactions and potential health benefits: An updated review
    Food Frontiers. Dec 30, 2020. [doi: 10.1002/fft2.56]

  2. Zengul AG, Demark-Wahnefried W, Barnes S, Morrow CD, Bertrand B, Berryhill TF, Frugé AD.
    Associations Between Dietary Fiber, the Fecal Microbiota and Estrogen Metabolism in Postmenopausal Women With Breast Cancer
    Nutr Cancer. 2020 Jun 26:1-10. [doi: 10.1080/01635581.2020.1784444] Online ahead of print.PMID: 32590914

  3. Manvi Sharma, Itika Arora, Matthew L. Stoll, Yuanyuan Li, Casey D. Morrow, Stephen Barnes, Taylor F. Berryhill, Shizhao Li, Trygve O. Tollefsbol
    Nutritional combinatorial impact on the gut microbiota and plasma short-chain fatty acids levels in the prevention of mammary cancer in Her2/neu estrogen receptor-negative transgenic mice
    [doi: 10.1101/2020.06.08.139865]

  4. Sharma NS, Vestal G, Wille K, Patel KK, Tipparaju S, Patel K, Menon S, Gaggar A, Xu X, Tousif S, Cheng F, Barnes S, Wilson LS, Lal CV, Dishaw L, Weill D, Dunning J, Hayes, Jr. D, Deshane JS, Liu R.
    Differences in airway microbiome and metabolome of single lung transplant recipients.
    J Clin Invest. 2019 Oct 14. pii: 127959. 2020 May 6;21(1):104. Respir Res. [PMC7201609] [doi:10.1186/s12931-020-01367-3 ]

  5. Hoffman JM, Poonawalla A, Icyuz M, Swindell WR, Wilson L, Barnes S, Sun LY.
    Transcriptomic and metabolomic profiling of long-lived growth hormone releasing hormone knock-out mice: evidence for altered mitochondrial function and amino acid metabolism.
    Aging (Albany NY). 2020 Feb 23;12. [doi: 10.18632/aging.102822] [Epub ahead of print] PMID:32091406

  6. Barnes S
    Overview of Experimental Methods and Study Design in Metabolomics, and Statistical and Pathway Considerations.
    Methods Mol Biol. 2020;2104:1-10. [doi: 10.1007/978-1-0716-0239-3_1] PMID:31953809

  7. Prasain, Jeevan K., Grubbs, Clinton, Barnes, Stephen.
    Cranberry anti-cancer compounds and their uptake and metabolism: An updated review.
    Journal of Berry Research, vol. 10, no. 1, pp. 1-10, 2020. [DOI: 10.3233/JBR-180370]

2019 Publications
  1. Larson-Casey JL, Vaid M, Gu L, He C, Cai GQ, Ding Q, Davis D, Berryhill TF, Wilson LS, Barnes S, Neighbors JD, Hohl RJ, Zimmerman KA, Yoder BK, Longhini ALF, Hanumanthu VS, Surolia R, Antony VB, Carter AB.
    Increased flux through the mevalonate pathway mediates fibrotic repair without injury.
    J Clin Invest. 2019 Nov 1;129(11):4962-4978. pii: 127959. [doi: 10.1172/JCI127959] [Epub ahead of print] [PMID: 31609245]

  2. Rainey K, Wilson L, Barnes S, Wu H.
    Quantitative Proteomics Uncovers the Interaction between a Virulence Factor and Mutanobactin Synthetases in Streptococcus mutans.
    mSphere. 2019 Sep 25;4(5). pii: e00429-19. [doi: 10.1128/mSphere.00429-19] [PMID: 31554721]

  3. Heaven MR, Wilson L, Barnes S, Brenner M.
    Relative stabilities of wild type and mutant glial fibrillary acidic protein in patients with Alexander disease.
    J Biol Chem. 2019 Sep 4. pii: jbc.RA119.009777. doi: 10.1074/jbc.RA119.009777. [Epub ahead of print] PMID: 31484723

  4. Brad Griesenauer, Tuan M. Tran, Kate R. Fortney, Diane M. Janowicz, Paula Johnson, Hongyu Gao, Stephen Barnes, Landon S. Wilson, Yunlong Liu, Stanley M. Spinola.
    Determination of an Interaction Network between an Extracellular Bacterial Pathogen and the Human Host.
    mBio. [doi: 10.1128/mBio.01193-19]

  5. Chen J, Nichols KK, Wilson L, Barnes S, Nichols JJ.
    Untargeted lipidomic analysis of human tears: A new approach for quantification of O-acyl-omega hydroxy fatty acids.
    Ocul Surf. 2019 Feb 25. pii: S1542-0124(18)30352-5. doi: 10.1016/j.jtos.2019.02.004. [Epub ahead of print] Review. [PMID: 30818035]
2018 Publications
  1. Lin VY, Fain MD, Jackson PL, Berryhill TF, Wilson LS, Mazur M, Barnes SJ, Blalock JE, Raju SV, Rowe SM.
    Vaporized E-Cigarette Liquids Induce Ion Transport Dysfunction in Airway Epithelia.
    Am J Respir Cell Mol Biol. 2018 Dec 21. [doi: 10.1165/rcmb.2017-0432OC] [Epub ahead of print] [PMID: 30576219]

  2. Chumley P, Zhou J, Mrug S, Chacko BK, Parant JM, Challa AK, Wilson LS, Berryhill TF, Barnes S, Kesterson RA, Bell PD, Darley-Usmar VM, Yoder BK, Mrug M.
    Truncating PKHD1 and PKD2 mutations alter energy metabolism.
    Am J Physiol Renal Physiol. 2018 Dec 19. [doi: 10.1152/ajprenal.00167.2018] [Epub ahead of print] [PMID: 30566001]

  3. Boohaker L, Halloran B, Wilson L, Berryhill T, Barnes S, Griffin R, Askenazi D.
    Absorbent materials to collect urine can affect proteomics and metabolomic biomarker concentrations.
    Clin Chem Lab Med. 2018 Oct 31. pii: /j/cclm.ahead-of-print/cclm-2018-0931/cclm-2018-0931.xml. [doi: 10.1515/cclm-2018-0931] [Epub ahead of print] No abstract available. [ PMID: 30375345]

  4. Yarar-Fisher C, Kulkarni A, Li J, Farley P, Renfro C, Aslam H, Bosarge P, Wilson L, Barnes S.
    Evaluation of a ketogenic diet for improvement of neurological recovery in individuals with acute spinal cord injury: a pilot, randomized safety and feasibility trial.
    Spinal Cord Ser Cases. 2018 Sep 21;4:88. doi: 10.1038/s41394-018-0121-4. eCollection 2018. [ PMID: 30275980]

  5. Lal CV, Kandasamy J, Dolma K, Ramani M, Kumar R, Wilson L, Aghai ZH, Barnes S, Blalock JE, Gaggar A, Bhandari V, Ambalavanan N.
    Early Airway Microbial Metagenomic and Metabolomic Signatures are Associated with Development of Severe Bronchopulmonary Dysplasia.
    Am J Physiol Lung Cell Mol Physiol. 2018 Aug 16. [doi: 10.1152/ajplung.00085.2018] [Epub ahead of print] [ PMID: 30113227]

  6. Hough KP, Wilson LS, Trevor JL, Strenkowski JG, Maina N, Kim YI, Spell ML, Wang Y, Chanda D, Dager JR, Sharma NS, Curtiss M, Antony VB, Dransfield MT, Chaplin DD, Steele C, Barnes S, Duncan SR, Prasain JK, Thannickal VJ, Deshane JS.
    Unique Lipid Signatures of Extracellular Vesicles from the Airways of Asthmatics.
    Sci Rep. 2018 Jul 9;8(1):10340. [doi: 10.1038/s41598-018-28655-9] [ PMID: 29985427]

  7. Redmann M, Benavides GA, Wani WY, Berryhill TF, Ouyang X, Johnson MS, Ravi S, Mitra K, Barnes S, Darley-Usmar VM, Zhang J.
    Methods for assessing mitochondrial quality control mechanisms and cellular consequences in cell culture.
    Redox Biol. 2018 Jul;17:59-69. doi: 10.1016/j.redox.2018.04.005. Epub 2018 Apr 10. [ PMID: 29677567

  8. Kain V, Ingle KA, Kabarowski J, Barnes S, Limdi NA, Prabhu SD, Halade GV.
    Genetic deletion of 12/15 lipoxygenase promotes effective resolution of inflammation following myocardial infarction.
    J Mol Cell Cardiol. 2018 May;118:70-80. [doi: 10.1016/j.yjmcc.2018.03.004] Epub 2018 Mar 8.

  9. Kim T, Nason S, Holleman C, Pepin M, Wilson L, Berryhill TF, Wende AR, Steele C, Young ME, Barnes S, Drucker DJ, Finan B, DiMarchi R, Perez-Tilve D, Tschoep M, Habegger KM.
    Glucagon-receptor Signaling Regulates Energy Metabolism Via Hepatic Farnesoid X Receptor and Fibroblast Growth Factor 21.
    Diabetes. 2018 Jun 20. pii: db171502. [doi: 10.2337/db17-1502] [Epub ahead of print] [ PMID: 29925501]
2017 Publications
  1. Paul B, Royston KJ, Li Y, Stoll ML, Skibola CF, Wilson LS, Barnes S, Morrow CD, Tollefsbol TO.
    Impact of genistein on the gut microbiome of humanized mice and its role in breast tumor inhibition.
    PLoS One. 2017 Dec 21;12(12):e0189756. [doi: 10.1371/journal.pone.0189756] eCollection 2017. [ PMID: 29267377]

  2. Ge J, Cui H, Xie N, Banerjee S, Guo S, Dubey S, Barnes S, Liu G.
    Glutaminolysis Promotes Collagen Translation and Stability via α-ketoglutarate Mediated mTOR Activation and Proline Hydroxylation.
    Am J Respir Cell Mol Biol. 2017 Oct 11. [ doi: 10.1165/rcmb.2017-0238OC] [Epub ahead of print] [PMID:29019707]

  3. Myers OD, Sumner SJ, Li S, Barnes S, Du X.
    Detailed Investigation and Comparison of the XCMS and MZmine 2 Chromatogram Construction and Chromatographic Peak Detection Methods for Preprocessing Mass Spectrometry Metabolomics Data.
    Anal Chem. 2017 Sep 5;89(17):8689-8695. [doi: 10.1021/acs.analchem.7b01069] Epub 2017 Aug 17. [PMID: 28752757]

  4. Myers OD, Sumner SJ, Li S, Barnes S, Du X.
    One Step Forward for Reducing False Positive and False Negative Compound Identifications from Mass Spectrometry Metabolomics Data: New Algorithms for Constructing Extracted Ion Chromatograms and Detecting Chromatographic Peaks.
    Anal Chem. 2017 Sep 5;89(17):8696-8703. doi: 10.1021/acs.analchem.7b00947] Epub 2017 Aug 17. [PMID: 28752754]

  5. Prasain JK, Wilson LS, Arabshahi A, Grubbs C, Barnes S.
    Mass spectrometric evidence for the modification of small molecules in a cobalt-60-irradiated rodent diet.
    J Mass Spectrom. 2017 Aug;52(8):507-516. [doi: 10.1002/jms.3950] Erratum in: J Mass Spectrom. 2017 Oct;52(10 ):707.
    [ PMID: 28544323]

  6. Redmann M, Benavides GA, Berryhill TF, Wani WY, Ouyang X, Johnson MS, Ravi S, Barnes S, Darley-Usmar VM, Zhang.
    Inhibition of autophagy with bafilomycin and chloroquine decreases mitochondrial quality and bioenergetic function in primary neurons.
    J. Redox Biol. 2017 Apr;11:73-81. [doi: 10.1016/j.redox.2016.11.004] Epub 2016 Nov 18. [PMID:27889640]

  7. Jordan SJ, Olson KM, Barnes S, Wilson LS, Berryhill TF, Bakshi R, Brown LT, Press CG, Geisler WM.
    Lower Levels of Cervicovaginal Tryptophan are Associated with Natural Clearance of Chlamydia in Women.
    J Infect Dis. 2017 May 17. [Epub ahead of print] [doi: 10.1093/infdis/jix240] [PMID:28520912]

  8. Shamik Das, William P. Jackson, Jeevan K. Prasain, Ann Hanna, Sarah K. Bailey, J. Allan Tucker, Sejong Bae, Landon S. Wilson, Rajeev S. Samant, Stephen Barnes & Lalita A. Shevde
    Loss of Merlin induces metabolomic adaptation that engages dependence on Hedgehog signaling.
    Scientific Reports 7, Article number: 40773 [doi:10.1038/srep40773]

2016 Publications
  1. Redmann M, Benavides GA, Berryhill TF, Wani WY, Ouyang X, Johnson MS, Ravi S, Barnes S, Darley-Usmar VM, Zhang J.
    Inhibition of autophagy with bafilomycin and chloroquine decreases mitochondrial quality and bioenergetic function in primary neurons.
    Redox Biol. 2016 Nov 18;11:73-81. doi: 10.1016/j.redox.2016.11.004. [Epub ahead of print] [ PMID: 27889640]

  2. Singh A, Rahman T, Bartiss R, Arabshahi A, Prasain J, Barnes S, Musteata FM, Sellati TJ.
    Lipoxin A4, a 5-lipoxygenase pathway metabolite, modulates immune response during acute respiratory tularemia.
    J Leukoc Biol. 2016 Sep 14. pii: jlb.4A0815-365RR. [Epub ahead of print] [ PMID: 27630217]

  3. Jeevan K. Prasain, Rajani Rajbhandari, Adam B. Keeton, Gary A. Piazzab and Stephen Barnes.
    Metabolism and growth inhibitory activity of cranberry derived flavonoids in bladder cancer cells.
    Food Funct., 2016, Advance Article, [ doi: 10.1039/C6FO00499G]

  4. Raju SV, Lin VY, Liu L, McNicholas CM, Karki S, Sloane PA, Tang L, Jackson PL, Wang W, Wilson L, Macon KJ, Mazur M, Kappes J, DeLucas LJ, Barnes S, Kirk K, Tearney GT, Rowe SM.
    The CFTR Potentiator Ivacaftor Augments Mucociliary Clearance Abrogating CF
    TR Inhibition by Cigarette Smoke.
    Am J Respir Cell Mol Biol. 2016 Sep 1. [Epub ahead of print] [PMID:27585394]

  5. McClure ML, Barnes S, Brodsky JL, Sorscher EJ.
    Trafficking and Function of the Cystic Fibrosis Transmembrane Conductance Regulator: A Complex Network of Post-Translational Modifications.
    Am J Physiol Lung Cell Mol Physiol. 2016 Jul 29:ajplung.00431.2015. [doi: 10.1152/ajplung.00431.2015] [Epub ahead of print] [PMID: 27474090]

  6. Barnes S, Benton HP, Casazza K, Cooper SJ, Cui X, Du X, Engler J, Kabarowski JH, Li S, Pathmasiri W, Prasain JK, Renfrow MB, Tiwari HK.
    Training in metabolomics research. I. Designing the experiment, collecting and extracting samples and generating metabolomics data.
    J Mass Spectrom. 2016 Jul;51(7):461-75. [doi: 10.1002/jms.3782] [ PMID: 27434804]

  7. Chacko BK, Wall SB, Kramer PA, Ravi S, Mitchell T, Johnson MS, Wilson L, Barnes S, Landar A, Darley-Usmar VM.
    Pleiotropic effects of 4-hydroxynonenal on oxidative burst and phagocytosis in neutrophils.
    Redox Biol. 2016 Jun 23;9:57-66. [doi: 10.1016/j.redox.2016.06.003] [Epub ahead of print] [ PMID: 27393890]

  8. Heaven MR, Flint D, Randall SM, Sosunov AA, Wilson L, Barnes S, Goldman JE, Muddiman DC, Brenner M.
    Composition of Rosenthal Fibers, the Protein Aggregate Hallmark of Alexander Disease.
    J Proteome Res. 2016 Jul 1;15(7):2265-82. [doi: 10.1021/acs.jproteome.6b00316] Epub 2016 Jun 2. [ PMID: 27193225]

  9. Rao S, Walters KB, Wilson L, Chen B, Bolisetty S, Graves D, Barnes S, Agarwal A, Kabarowski JH.
    Early lipid changes in acute kidney injury using SWATH lipidomics coupled with MALDI tissue imaging.
    Am J Physiol Renal Physiol. 2016 Feb 24:ajprenal.00100.2016. doi: 10.1152/ajprenal.00100.2016. [Epub ahead of print] [PMID: 26911846]

  10. Xie X, Wang X, Mick GJ, Kabarowski JH, Shay Wilson L, Barnes S, Walcott GP, Luo X, McCormick K.
    Effect of n-3 and n-6 Polyunsaturated Fatty Acids on Microsomal P450 Steroidogenic Enzyme Activities and In Vitro Cortisol Production in Adrenal Tissue from Male Yorkshire Boars.
    Endocrinology. 2016 Feb 18:en20151831. [Epub ahead of print] [PMID: 26889941]

2015 Publications
  1. Pawlowski JW, Martin BR, McCabe GP, McCabe L, Jackson GS, Peacock M, Barnes S, Weaver CM.
    Impact of equol-producing capacity and soy-isoflavone profiles of supplements on bone calcium retention in postmenopausal women: a randomized crossover trial.
    Am J Clin Nutr. 2015 Sep;102(3):695-703. [doi: 10.3945/ajcn.114.093906]. Epub 2015 Aug 5. [ PMID: 26245807]

  2. Brossier NM1, Prechtl AM, Longo JF, Barnes S, Wilson LS, Byer SJ, Brosius SN, Carroll SL.
    Classic Ras Proteins Promote Proliferation and Survival via Distinct Phosphoproteome Alterations in Neurofibromin-Null Malignant Peripheral Nerve Sheath Tumor Cells.
    J Neuropathol Exp Neurol. 2015 Jun;74(6):568-86. [doi: 10.1097/NEN.0000000000000201] [PMID: 25946318]

  3. Lopez EL, Kabarowski JH, Ingle KA, Kain V, Barnes S, Crossman D, Lindsey ML, Halade GV.
    Obesity superimposed on aging magnifies inflammation and delays the resolving response after myocardial infarction.
    Am J Physiol Heart Circ Physiol. 2015 Feb 15;308(4):H269-80. [doi: 10.1152/ajpheart.00604.2014] Epub 2014 Dec 5. [ PMID: 25485899]

  4. Harper VM, Oh JY, Stapley R, Marques MB, Wilson L, Barnes S, Sun CW, Townes T, Patel RP.
    Peroxiredoxin-2 Recycling Is Inhibited During Erythrocyte Storage.
    Antioxid Redox Signal. 2015 Feb 1;22(4):294-307. [doi: 10.1089/ars.2014.5950] Epub 2014 Nov 10. [PMID: 25264713]

2014 Publications
  1. Lopez EL, Kabarowski JH, Ingle KA, Kain V, Barnes S, Crossman D, Lindsey ML, Halade GV.
    PUFA-induced obesity magnifies inflammation and delays the resolving response following myocardial infarction in aging.
    Accepted for publication in AJP-Heart and Circulatory.

  2. Kramer PA, Chacko BK, Ravi S, Johnson MS, Mitchell T, Barnes S, Arabshahi A, Dell'Italia LJ, George DJ, Steele C, George JF, Darley-Usmar VM, Melby SJ.
    Hemoglobin-associated oxidative stress in the pericardial compartment of postoperative cardiac surgery patients.
    Lab Invest. 2014 Dec 1. [doi: 10.1038/labinvest.2014.144] [Epub ahead of print] [PMID: 25437645]

  3. Nakatsu CH, Armstrong A, Clavijo AP, Martin BR, Barnes S, Weaver CM.
    Fecal bacterial community changes associated with isoflavone metabolites in postmenopausal women after soy bar consumption.
    PLoS One. 2014 Oct 1;9(10):e108924. [doi: 10.1371/journal.pone.0108924] eCollection 2014] [PMID: 25271941]

  4. Harper VM, Oh JY, Stapley R, Marques MB, Wilson L, Barnes S, Sun CW, Townes T, Patel RP.
    Peroxiredoxin-2 Recycling Is Inhibited During Erythrocyte Storage.
    Antioxid Redox Signal. 2014 Nov 10. [Epub ahead of print] [PMID: 25264713] [PubMed - as supplied by publisher]

  5. Wilson L, Arabshahi A, Simons B, Prasain JK, Barnes S.
    Improved high sensitivity analysis of polyphenols and their metabolites by nano-liquid chromatography-mass spectrometry.
    Arch Biochem Biophys. 2014 Oct 1;559:3-11. [doi: 10.1016/] [PMID: 24967696]

  6. Glinskii OV, Li F, Wilson LS, Barnes S, Rittenhouse-Olson K, Barchi JJ Jr, Pienta KJ, Glinsky VV.
    Endothelial integrin α3β1 stabilizes carbohydrate-mediated tumor/endothelial cell adhesion and induces macromolecular signaling complex formation at the endothelial cell membrane.
    Oncotarget. 2014 Mar 15;5(5):1382-9. [PMID: 24675526] [PMCID: PMC4012737]

  7. Legette LL, Prasain J, King J, Arabshani A, Barnes S, Weaver CM.
    Pharmacokinetics of Equol, a Soy Isoflavone Metabolite, Changes with Form of Equol (Dietary vs. Intestinal Production) in Ovariectomized Rats.
    J Agric Food Chem. 2014 Jan 21 [PMID: 24446705]

2013 Publications
  1. Cutts JK, Peavy TR, Moore DR, Prasain J, Barnes S, Kim H.
    Ovariectomy lowers urine levels of unconjugated (+)-catechin, (-)-epicatechin, and their methylated metabolites in rats fed grape seed extract.
    Horm Mol Biol Clin Investig. 2013 Dec;16(3):129-38. [doi: 10.1515/hmbci-2013-0044] [ PMID: 25436866]

  2. Whitehouse-Tedd K, Cave N, Ugarte C, Tucker L, Prasain JK, Arabshahi A, Barnes S, Hendriks W, Thomas D.
    Isoflavone metabolism in domestic cats (Felis catus): comparison of plasma metabolites detected after ingestion of two different dietary forms of genistein and daidzein.
    J Anim Sci. 2013 Mar;91(3):1295-306. [PMID: 23307849] [doi: 10.2527/jas.2011-4812]

  3. Viera L, Radmilovich M, Vargas MR, Dennys CN, Wilson L, Barnes S, Franco MC, Beckman JS, Estévez AG.
    Temporal patterns of tyrosine nitration in embryo heart development.
    Free Radic Biol Med. 2013 Feb;55:101-8. [PMID: 23195686]

  4. Franco MC, Ye Y, Refakis C, Feldman JL, Stokes AL, Basso M, Melero Fernández de Mera RM, Calingasan NY, Kiaei M, Barnes S, Beal MF, Beckman JS, Mehl R, Estévez AG.
    Nitration of Hsp90 induces cell death.
    PNAS, 110:E1102-11. [PMCID: PMC3607042]

  5. Bolisetty S, Traylor AM, Zarjou A, Johnson MS, Benavides GA, Ricart K, Boddu R, Moore RD, Landar A, Barnes S, Darley-Usmar VM, Agarwal A.
    Mitochondria-targeted heme oxygenase-1 decreases oxidative stress in renal epithelial cells.
    Am J Physiol Renal Physiol. 2013 305:F255-F264 [PMID: 23720344]

  6. Mitchell T, Johnson MS, Ouyang O, Chacko BK, Mitra K, Lei X, Gai Y, Moore DR, Barnes S, Zhang J, Koizumi A, Ramanadham S, Darley-Usmar. VM.
    Dysfunctional mitochondrial bioenergetics and oxidative stress in Akita+/Ins2-derived β-cells.
    Am J Physiol Endocrinol Metab. 2013 Jul 2. [Epub ahead of print] [PMID: 23820623]

  7. Ulasova E, Perez J, Hill BG, Dubuisson JG, Bradley WE, Garber DW, Landar A, Barnes S, Prasain J, Parks DA, Dell’Italia LJ, Darley-Usmar VM.
    Quercetin prevents left ventricular hypertrophy in the Apo E knockout mouse.
    Redox Biol. 2013 Jul 16;1(1):381-6. [PMID: 24024175]

  8. Wetta LA, Biggio JR, Cliver S, Abramovici A, Barnes S, Tita AT.
    Is Midtrimester Vitamin D Status Associated with Spontaneous Preterm Birth and Preeclampsia?
    Am J Perinatol. 2013 Sep 10. [Epub ahead of print] [PMID: 24022379]

  9. Chiquito Crasto, Chandrahas Narne, Mikako Kawai, Landon Wilson and Stephen Barnes
    MRMPath and MRMutation, Facilitating Discovery of Mass Transitions for Proteotypic Peptides in Biological Pathways Using a Bioinformatics Approach
    Advances in Bioinformatics, vol. 2013, Article ID 527295, 10 pages, 2013. [doi: 10.1155/2013/527295]

  10. Jeevan K. Prasain, Alireza Arabshahi, Pam R. Taub, Scott Sweeney, Ray Moore, J. Daniel Sharer, Stephen Barnes.
    Simultaneous quantification of F2-isoprostanes and prostaglandins in human urine by liquid chromatography tandem-mass spectrometry
    Journal of Chromatography B. Volumes 913–914, 15 January 2013, Pages 161–168 [doi: 10.1016/j.jchromb.2012.12.009]
2012 Publications
  1. Cheng GC, Chen DT, Chen JJ, Soong SJ, Lamartiniere C, Barnes S.
    A chain reaction approach to modelling gene pathways.
    Transl Cancer Res. 2012 Aug 1;1(2):61-73. Epub 2012 May 31. [ PMID: 22943042]

  2. Higdon A, Landar A, Barnes S, Darley-Usmar VM.
    The Electrophile Responsive Proteome: Integrating Proteomics and Lipidomics with Cellular Function.
    Antioxidants & Redox Signaling, 2012 Dec 1;17(11):1580-9. Epub 2012 Apr 18. [PMID: 22352679]

  3. Wong WW, Taylor AA, E. Smith EO, Barnes S, Hachey DL.
    Effect of soy isoflavone supplementation on nitric oxide metabolism and blood pressure in menopausal women.
    American Journal of Clinical Nutrition. American Journal of Clinical Nutrition, 2012 Jun;95(6):1487-94. [PMID: 22552034]

  4. Li H, Cao, Z, Moore DR, Patricia L. Jackson PL, Barnes S, Lambeth JD, Thannickal VJ, Cheng G.
    Microbicidal Activity of VPO1 in Human Plasma via Generation of HOCl. Li H, Cao, Z, Moore DR, Patricia L. Jackson PL, Barnes S, Lambeth JD, Thannickal VJ, Cheng G. Microbicidal Activity of VPO1 in Human Plasma via Generation of HOCl.
    Infection and Immunity. 2012 Jul;80(7):2528-37. Epub 2012 Apr 23 [PMID: 22526679]

  5. McClure M, DeLucas LJ, Wilson L, Ray M, Rowe SM, Wu X, Dai Q, Hong JS, Sorscher EJ, Kappes JC, Barnes S.
    Purification of CFTR for mass spectrometry analysis: identification of palmitoylation and other post-translational modifications. Protein Eng Des Sel. 2012 Jan;25(1):7-14. Epub 2011 Nov 25. [PMID: 22119790] [PMCID: PMC3276306]
2011 Publications
  1. Whitehouse-Tedd KM, Cave NJ, Ugarte CE, Waldron LA, Prasain JK, Arabshahi A, Barnes S, Thomas DG.
    Dietary isoflavone absorption, excretion, and metabolism in captive cheetahs (Acinonyx jubatus).
    J Zoo Wildl Med. 2011 Dec;42(4):658-70. [PMID: 22204061] [doi:10.1638/2011-0060.1]

  2. Li FJ, Kubagawa Y, McCollum MK, Wilson L, Motohashi T, Bertoli LF, Barton JC, Barnes S, Davis RS, Kubagawa H.
    Enhanced levels of both membrane-bound and soluble forms of IgM Fc receptor in patients with chronic lymphocytic leukemia.
    Blood. 2011 Sep 9. [Epub ahead of print] [PMID: 21908424]

  3. Barnes S, Prasain J, D'Alessandro T, Arabshahi A, Botting N, Lila MA, Jackson G, Janle EM, Weaver CM.
    The metabolism and analysis of isoflavones and other dietary polyphenols in foods and biological systems.
    Food Funct. 2011 May 25;2(5):235-44. [PMID: 21779561]

  4. Li F, Glinskii OV, Zhou J, Wilson LS, Barnes S, Anthony DC, Glinsky VV.
    Identification and analysis of signaling networks potentially involved in breast carcinoma metastasis to the brain.
    PLoS One. 2011;6(7):e21977. [PMID: 21779361]

  5. Legette LL, Lee WH, Martin BR, Story JA, Arabshahi A, Barnes S, Weaver CM.
    Genistein, a phytoestrogen, improves total cholesterol, and Synergy, a prebiotic, improves calcium utilization, but there were no synergistic effects.
    Menopause. 2011 Jun 7. [Epub ahead of print] [PMID: 21659907]

  6. Frölich MA, Arabshahi A, Katholi C, Prasain J, Barnes S.
    Hemodynamic characteristics of midazolam, propofol, and dexmedetomidine in healthy volunteers.
    J Clin Anesth. 2011 May;23(3):218-23. [PMID: 21570617]

  7. Mirza S, Wilson L, Benjamin WH Jr, Novak J, Barnes S, Hollingshead SK, Briles DE.
    Serine protease PrtA from Streptococcus pneumoniae plays a role in the killing of S. pneumoniae by apolactoferrin.
    Infect Immun. 2011 Mar 21. [Epub ahead of print] [PMID:21422179]

  8. Anderson JT, Zeng M, Li Q, Stapley R, Moore DR 2nd, Chenna B, Fineberg N, Zmijewski J, Eltoum IE, Siegal GP, Gaggar A, Barnes S, Velu SE, Thannickal VJ, Abraham E, Patel RP, Lancaster JR Jr, Chaplin DD, Dransfield MT, Deshane JS.
    Elevated levels of NO are localized to distal airways in asthma.
    Free Radic Biol Med. 2011 Mar 16. [Epub ahead of print] [PMID:21419218]

  9. Rajbhandari, R, Peng, N, Moore, DR, Arabshahi, A, Wyss, JM, Barnes S, Jeevan K. Prasain JK.
    Determination of cranberry phenolic metabolites in rats by liquid chromatography-tandem mass spectrometry.
    J Agric Food Chem, 2011 [PMID: 21634376]

  10. Floyd KA, Stella DR, Wang CC, Laurentz S, McCabe GP, Srivastava OP, Barnes S.
    Genistein and genistein-containing dietary supplements accelerate the early stages of cataractogenesis in the male ICR/f rat.
    Exp Eye Res. 2011 Feb;92(2):120-7. [PMID: 21147102]

2010 Publications
  1. Barnes S, Kim H.
    Cautions and research needs identified at the equol, soy, and menopause research leadership conference.
    J Nutr. 2010 Jul;140(7):1390S-4S. [PMID: 20505015]

  2. Virk-Baker MK, Nagy TR, Barnes S.
    Role of Phytoestrogens in Cancer Therapy.
    Planta Med. 2010 Aug;76(11):1132-42. [PMID: 20597043]

  3. Sun D, Zhuang X, Xiang X, Liu Y, Zhang S, Liu C, Barnes S, Grizzle W, Miller D, Zhang HG.
    A Novel Nanoparticle Drug Delivery System: The Anti-inflammatory Activity of Curcumin Is Enhanced When Encapsulated in Exosomes.
    Mol Ther. 2010 Sep;18(9):1606-14. [PMID: 20571541]

  4. Raska M, Takahashi K, Czernekova L, Zachova K, Hall S, Moldoveanu Z, Elliott MC, Wilson L, Brown R, Jancova D, Barnes S, Vrbkova J, Tomana M, Smith PD, Mestecky J, Renfrow MB, Novak J.
    Glycosylation patterns of HIV-1 gp120 depend on the type of expressing cells and affect antibody recognition.
    J Biol Chem. 2010. 285(27):20860-9. [PMID: 20439465]

  5. Stella DR, Floyd KA, Grey AC, Renfrow MB, Schey KL, Barnes S.
    Tissue localization and solubilities of {alpha}A-crystallin and its numerous C-terminal truncation products in pre- and post-cataractous ICR/f rat lenses.
    Invest Ophthalmol Vis Sci. 2010 Oct;51(10):5153-61. [PMID: 20435586]

  6. Prasain JK, Arabshahi A, Moore DR 2nd, Greendale GA, Wyss JM, Barnes S.
    Simultaneous determination of 11 phytoestrogens in human serum using a 2 min liquid chromatography/tandem mass spectrometry method.
    J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Apr 15;878(13-14):994-1002. Epub 2010 Mar 6. [PMID: 20346741]

  7. Barnes S.
    The biochemistry, chemistry and physiology of the isoflavones in soybeans and their food products.
    Lymphat Res Biol. 2010. 8(1):89-98. [DOI:10.1089/lrb.2009.0030] [PMID: 20235891]

2009 Publications
  1. Pyle LC, Fulton JC, Sloane PA, Backer K, Mazur M, Prasain J, Barnes S, Clancy JP, Rowe SM.
    Activation of CFTR by the Flavonoid Quercetin: Potential Use as a Biomarker of ΔF508 CFTR Rescue
    Am J Respir Cell Mol Biol. 2009 Dec 30. [DOI:10.1165/rcmb.2009-0281OC] [PMID: 20042712]

  2. Peng N, Prasain JK, Dai Y, Moore R, Arabshahi A, Barnes S, Carlson S, Wyss JM.
    Chronic dietary kudzu isoflavones improve components of metabolic syndrome in stroke-prone spontaneously hypertensive rats.
    J Agric Food Chem. 26;57(16):7268-73, 2009.. [DOI:10.1021/jf901169y] [PMID: 19938872]

  3. Smith DL Jr, Nagy TR, Wilson LS, Dong S, Barnes S, Allison DB.
    The Effect of Mannan Oligosaccharide Supplementation on Body Weight Gain and Fat Accrual in C57Bl/6J Mice.
    Obesity. 2009 Oct 1. Epub 2009 Oct 1. [DOI: 10.1038/oby.2009.308] [PMID: 19798073]

  4. Wong WW, Lewis RD, Steinberg FM, Murray MJ, Cramer MA, Amato P, Young RL, Barnes S, Ellis KJ, Shypailo RJ, Fraley JK, Konzelmann KL, Fischer JG, Smith EO.
    Soy isoflavone supplementation and bone mineral density in menopausal women: a 2-y multicenter clinical trial.
    Am J Clin Nutr. 2009 Sep 16. Epub 2009 Sep 16. [DOI: 10.3945/ajcn.2009.28001] [PMID: 19759166]

  5. Legette LL, Martin BR, Shahnazari M, Lee WH, Helferich WG, Qian J, Waters DJ, Arabshahi A, Barnes S, Welch J, Bostwick DG, Weaver CM.
    Supplemental dietary racemic equol has modest benefits to bone but has mild uterotropic activity in ovariectomized rats.
    Journal of Nutrition,, 139(10):1908-13. [DOI: 10.3945/jn.109.108225] [PMID: 19710157]

  6. Cox KB, Liu J, Tian L, Barnes S, Yang Q, Wood PA.
    Cardiac hypertrophy in mice with long-chain acyl-CoA dehydrogenase or very long-chain acyl-CoA dehydrogenase deficiency.
    Laboratory Investigation, 2009 Sep 7. [ DOI: 10.1038/labinvest.2009.86] [PMID: 19736549]

  7. Weaver CM, Martin BR, Jackson GS, McCabe GP, Nolan JR, McCabe LD, Barnes S, Reinwald S, Boris ME, Peacock M.
    Antiresorptive Effects of Phytoestrogen Supplements Compared to Estradiol or Risedronate in Postmenopausal Women Using 41Ca Methodology.
    J Clin Endocrinol Metab. 2009 Jul 7 [DOI: 10.1210/jc.2009-0332] [PMID: 19584189]

  8. Prasain JK, Peng N, Dai Y, Moore R, Arabshahi A, Wilson L, Barnes S, Michael Wyss J, Kim H, Watts RL.
    Liquid chromatography tandem mass spectrometry identification of proanthocyanidins in rat plasma after oral administration of grape seed extract.
    Phytomedicine, 16(2-3):233-43, 2009. [DOI: 10.1016/j.phymed.2008.08.006] [PMID: 19095430]

  9. Prasain JK, Peng N, Moore R, Arabshahi A, Barnes S, Wyss JM.
    Tissue distribution of puerarin and its conjugated metabolites in rats assessed by liquid chromatography-tandem mass spectrometry.
    Phytomedicine, 16(1); 65-71, 2009. [DOI: 10.1016/j.phymed.2008.09.004] [PMID: 19027277]

  10. Weaver CM, Barnes S, Wyss JM, Kim H, Morré DM, Morré DJ, Simon JE, Lila MA, Janle EM, Ferruzzi MG.
    Research Highlights from the Purdue-UAB Botanicals Research Center for Age Related Diseases.
    Pharm Biol. 2009 47(8):768-773. [PMID: 19890436] [DOI: 10.1080/13880200902988603]
2008 Publications
  1. Prasain JK, Arabshahi A, Moore R, Barnes S, Carroll SL.
    A liquid chromatography mass spectrometry assay for determination of PD168393, a specific and irreversible inhibitor of erbB membrane tyrosine kinases, in rat serum.
    Journal of Chromatogr B. 876(2): 219-224, 2008

  2. Barnes, S.
    Nutritional Genomics, Polyphenols, Diets, and Their Impact on Dietetics.
    Journal of the American Dietetic Association, 108(11):1888-95, 2008.

  3. Barnes, S., Shonsey, E.M., Eliuk, S.M., Stella, D., Mafia, K., Kim, H., Srivastava, O.P., Renfrow, M.B.
    High-resolution mass spectrometry analysis of protein oxidations and resultant loss of function.
    Biochemical Society Transactions, 36: 1037-1044, 2008.

  4. Kim, H., Cope, M., Herring, R., Robinson, G., Wilson, L., Page, G.P., Barnes, S.
    2D Difference Gel Electrophoresis of Prepubertal and Pubertal Rat Mammary Gland Proteomes.
    Journal of Proteome Research, 7 (11): 4638-4650, 2008.

  5. Prasain J.K., Jones, K., Moore, D.R., Barnes, S., Leahy, M., Roderick, R., Juliana, M.M. and Grubbs, C.J.
    Effect of cranberry juice concentrate on chemically-induced urinary bladder cancers.
    Oncology Reports 19: 1565-1570, 2008.

  6. Sarkar P, Sarkar S, Ramesh V, Kim H, Barnes S., Kulkarni A, Hall JC, Wilson BL, Thomas RL, Pellis NR, Ramesh GT.
    Proteomic Analysis of Mouse Hypothalamus under Simulated Microgravity.
    Neurochem Res. 33(11): 2335-41, 2008.

  7. Mafia K, Gupta R, Kirk M, Wilson L, Srivastava OP, Barnes S.
    UV-A-induced structural and functional changes in human lens deamidated alphaB-crystallin.
    Molecular Vision. 2008 14: 234-48, 2008.

  8. Barnes, S., Birt, D.F., Cassileth, B.R. Cefalu, W.T., Chilton, F.H., Farnsworth, N.R., Raskin, I., van Breemen, R.B., Weaver, C.M.
    Technologies and experimental approaches at the National Institutes of Health Botanical Research Centers.
    American Journal of Clinical Nutrition, 87(2): 476S-80S, 2008.

  9. Shonsey, E.M., Eliuk, S.M., Johnson, M.S., Barnes, S., Falany, C.N , Darley-Usmar, V.M. and Renfrow, M.B.
    Inactivation of human liver bile acid CoA:amino acid N-acyltransferase by the electrophilic lipid, 4-hydroxynonenal.
    Journal of Lipid Research, 49(2):282-94, 2008.

  10. Ashwell, M., Stone, E., Mathers, J., Barnes, S., Compston, J., Francis, R.M., Key, T., Cashman, K.D., Cooper, C., Khaw, K.T., Lanham-New, S., Macdonald, H., Prentice, A., Shearer, M. and Stephen, A.
    Nutrition and bone health projects funded by the UK Food Standards Agency: have they helped to inform public health policy?
    British Journal of Nutrition, 99: 198-205, 2008.

  11. Weaver, C.M., Barnes, S.,Wyss, J.M., Kim, H., Morré, D.M., Morré, D.J., Simon, J.E., Lila, M.A., Janle, E.M., Ferruzzi, M.G..
    Botanicals for age-related diseases: from field to practice.
    American Journal of Clinical Nutrition, 87(2): 493S-7S, 2008

Pre 2008 Publications


  1. Zhang, H-G., Kim, H., Liu, C., Yu, S., Wang, J, Zhang, L., Grizzle, W.E., Kimberly, R.P., and Barnes, S.
    Curcumin reverses breast tumor exosomes mediated immune suppression of NK cell tumor cytotoxicity.
    Biochimica et Biophysica Acta, 1773: 1116-1123, 2007.

  2. Kim H, Eliuk S, Deshane J, Meleth S, Sanderson T, Pinner A, Robinson G, Wilson L, Kirk M, Barnes S.
    2D gel proteomics: an approach to study age-related differences in protein abundance or isoform complexity in biological samples.
    Methods in Molecular Biology 371:349-391, 2007.

  3. Shannon M. Eliuk, Matthew B. Renfrow, Erin M. Shonsey, Stephen Barnes, and Helen Kim.
    Active Site Modifications of the Brain Isoform of Creatine Kinase by 4-Hydroxy-2-nonenal Correlate with Reduced Enzyme Activity: Mapping of Modified Sites by Fourier Transform-Ion Cyclotron Resonance Mass Spectrometry .
    Chemical Research in Toxicology, 20:1260-1268, 2007.

  4. Prasain, J.K., Barnes, S.
    Metabolism and bioavailability of flavonoids in chemoprevention: current analytical strategies and future prospectus.
    Molecular Pharmaceutics. 4 (6), 846-864, 2007.

  5. Cheong, J.M.K., Martin, B.R., Jackson, G.J., Elmore, D., McCabe, G.P., Nolan, J.R., Barnes, S., Peacock, M., and Weaver, C.M.
    Soy isoflavones do not affect bone resorption in postmenopausal women: A dose response study using a novel approach with 41Ca.
    Journal of Clinical Endocrinology & Medicine, 92: 577-582, 2007.

  6. Prasain, J., Peng, N., Acosta, E., Moore, D.R., Arabshahi, A., Meezan, E., Barnes, S., Wyss, J.M.
    Pharmacokinetic study of puerarin in rat serum by liquid chromatography tandem mass spectrometry.
    Biomedical Chromatography, 21(4): 410 - 414, 2007.

  7. Yaozu Ye, Celia Quijano, Kristine M. Robinson, Karina C. Ricart, Amy L. Strayer, Mary Anne Sahawneh, John J. Shacka, Marion Kirk, Stephen Barnes, Mary Ann Accavitti-Loper, Rafael Radi, Joseph S. Beckman, and Alvaro G. Estevez.
    Prevention of peroxynitrite-induced apoptosis of motor neurons and PC12 cells by tyrosine-containing peptides.
    Journal of Biological Chemistry, 282: 6324-6337, 2007.

  8. Chacko, B.K., Chandler, R.T., D¹Alessandro, T.L., Mundhekar, A., Khoo, N.K., Botting, N., Barnes, S., Patel, R.P.
    Anti-inflammatory effects of isoflavones are dependent on flow and human endothelial cell PPARg.
    Journal of Nutrition, 137: 351-356, 2007.

  9. Lee, S.A., Wen, W, Xiang, Y.B., Barnes, S., Liu, D.K., Cai, Q., Zheng, W., Shu, X.O.
    Assessment of dietary isoflavone intake among middle age Chinese men.
    Journal of Nutrition, 137: 1011-1016, 2007.

  10. Prasain, J.K., Reppert, A., Jones, K., Moore, D.R., II, Barnes, S., and Lila, M.A.
    Identification of isoflavone glycosides in Pueraria lobata cultures by tandem mass spectrometry.
    Phytochemical Analysis, Phytochemical Analysis 18:50-59, 2007.

  11. C. Sites, B. Cooper, M. Toth, A. Gastaldelli, A. Arabshahi, S. Barnes
    Effect of a daily supplement of soy protein on body composition and insulin secretion in postmenopausal women.
    Fertility and Sterility, 2007.

  12. Styles NA, Falany JL, Barnes S, Falany CN.
    Quantification and regulation of the subcellular distribution of bile acid coenzyme A:amino acid N-acyltransferase activity in rat liver.
    J Lipid Res. 48(6):1305-15, 2007


  1. Poonam Sarkar, Shubhashish Sarkar, Vani Ramesh, Barbara E. Hayes, Renard L. Thomas, Bobby L. Wilson, Helen Kim, Stephen Barnes, Anil Kulkarni, Neal Pellis, and Govindarajan T. Ramesh
    Proteomic Analysis of Mice Hippocampus in Simulated Microgravity Environment
    Journal of Proteome Research, 5, 548-553, 2006.

  2. Barnes, S., Prasain, J.K., Wang, C-C., Moore, D.R., II.
    Applications of LC-MS in the study of the uptake, distribution, metabolism and excretion of bioactive polyphenols from dietary supplements.
    Life Sciences, 78(18): 2054-9, 2006.

  3. Landar, A., Giles, N.M., Oh, J-Y., Foxwell, A., Kirk, M., Barnes, S., and Darley-Usmar, V.M.
    A sensitive method for the quantitative measurement of protein thiol modification in response to oxidative stress.
    Free Radical Biology and Medicine, 40, 459-468, 2006.

  4. Horn-Ross, P.L., Barnes, S., Lee, V.S., Collins, C.N., Reynolds, P., Lee, M.M., Stewart, S.L., Canchola, A.J., Wilson, L., Jones, K.
    Reliability and Validity of an Assessment of Usual Phytoestrogen Consumption.
    Cancer Causes and Control, 17:85-93, 2006.

  5. Helen Kim, Jessy Deshane, Stephen Barnes, Sreelatha Meleth
    Proteomics analysis of the actions of grape seed extract in rat brain: technological and biological implications for the study of the actions of psychoactive compounds.
    Life Sciences 78: 2060-2065, 2006.

  6. Helen Kim, Shannon Eliuk, and Jessy Deshane, Stephen Barnes, Sreelatha Meleth
    Nutriproteomics Approach to Understanding Dementia-Relevant Brain Protein Changes in Response to Grape Seed Extract, a Dietary Antioxidant
    Oxidative Stress and Age-Related Neurodegeneration edited by Yuan Luo
    CRC Press, 2006, Chapter 26, ISBN: 0849337259

  7. Huang, C.M., Wang, C.C., Kawai, M., Barnes, S., and Elmets, C.A.
    Surfactant sodium lauryl sulfate enhances skin vaccination: Molecular characterization via a novel technique using ultrafiltration capillaries and mass spectrometric proteomics.
    Molecular & Cellular Proteomics 5:523-532, 2006.

  8. Hsu, H.C., Zhou, T., Kim, H., Barnes, S., Yang, P., Wu, Q., Zhou, J., Freeman, B.A., Luo, M., Mountz, J.D.
    Production of a novel class of polyreactive pathogenic autoantibodies in BXD2 mice causes glomerulonephritis and arthritis.
    Arthritis & Rheumatism 54: 343-355, 2006.

  9. Huang, C.M., Wang, C-C., Kawai, M., Barnes, S., Elmets, C.A.
    In vivo protein sampling using capillary ultrafiltration semi-permeable hollow fiber and protein identification via mass spectrometry-based proteomics.
    J Chromatogr A, 1109: 144-151, 2006.

  10. Barnes, S.
    In the postgenomic era, can high-dimensional analyses deliver the answers we're looking for?
    Journal of Nutrition 136: 2709S-2710S, 2006.

  11. Huang CM, Ananthaswamy HN, Barnes S, Ma Y, Kawai M, Elmets CA.
    Mass spectrometric proteomics profiles of in vivo tumor secretomes: Capillary ultrafiltration sampling of regressive tumor masses.
    Proteomics 6: 6107-6116, 2006.

  12. Prasain, J.K., Reppert, A., Jones, K., Moore, D.R., II, Barnes, S., and Lila, M.A.
    Identification of Isoflavone Glycosides in Pueraria lobata Cultures by Tandem Mass Spectrometry.
    Phytochemical Analysis, 18: 50-59, 2006.

  13. Prasain, J.K., Xu, J., Kirk, M., Smith, M., Sfakianos, J., and Barnes, S.
    Differential biliary excretion of genistein metabolites following intraduodenal and intravenous infusion of genistin in female rats.
    Journal of Nutrition, 136:2975-2979, 2006.

  14. Huang CM, Wang CC, Barnes S, Elmets CA.
    In vivo detection of secreted proteins from wounded skin using capillary ultrafiltration probes and mass spectrometric proteomics.
    Proteomics. 6(21):5805-14, 2006.

  15. Kim K, Page GP, Beasley TM, Barnes S, Scheirer KE, Allison DB.
    A proposed metric for assessing the measurement quality of individual microarrays.
    BMC Bioinformatics. 23;7:35 2006.

  16. Liu C, Yu S, Zinn K, Wang J, Zhang L, Jia Y, Kappes JC, Barnes S, Kimberly RP, Grizzle WE, Zhang HG.
    Murine mammary carcinoma exosomes promote tumor growth by suppression of NK cell function.
    The Journal of Immunology. 1;176(3):1375-85, 2006.


  1. Barnes, S., Prasain, J.K.
    Current Progress in the use of Traditional Medicines and Nutraceuticals.
    Current Opinions in Plant Biology, 8: 324-328, 2005.

  2. Allison, D.B., Cutter, G., Poehlman E.T., Moore, D.R. II, Barnes, S.
    Exactly which synephrine alkaloids does Citrus Aurantium (bitter orange) contain?
    International Journal of Obesity, 29:443-446, 2005.

  3. Cai, J., Zhao, Y., Glasier, J., Cullen, D., Barnes, S., Turner, C., and Weaver, C.
    Comparative effect of soy protein, soy isoflavones and 17β-estradiol on bone metabolism in adult ovariectomized rats.
    Journal of Bone and Mineral Research, 20: 828-839, 2005.

  4. Prasain JK, Moore DR, Barnes S, van Kuijk FJGM.
    Electrospray Tandem Mass Spectrometric Analysis of Carotenoids and their Oxidation Products.
    Journal of Mass Spectrometry, 40: 916-923, 2005.

  5. Hedlund, T.E., Maroni, P.D., Ferucci, P.G., Dayton, R., Barnes, S., Jones, R., Moore, D.R., Ogden, L.G., Wähälä, K., Sackett, H.M, and Gray, K.J.
    Long-term dietary habits influence soy isoflavone metabolism in caucasian men: selective accumulation of isoflavonoids within prostatic fluid.
    Journal of Nutrition, 135: 1400-1406, 2005.

  6. Uppala, P.T., Roy, S.K., Tousson, A., Barnes, S., Uppala, G.R., and Eastmond, D.A.
    Induction of cell proliferation and hyperploidy/polyploidy in the mammary cells of DDT- and DMBA-treated pubertal rats.
    Environmental and Molecular Mutagenesis, 46(1):43-52, 2005.

  7. Chacko, B.K., Robert Chandler, R.T., Mundhekar, A., Pruitt, H.M., Kucik, D.F., Kevil, C.G., Barnes, S., Patel, R.P.
    Revealing anti-inflammatory mechanisms of soy-isoflavones by flow: modulation of leukocyte-endothelial cell interactions.
    American Journal of Physiology, 289: H908-H915, 2005.

  8. Yang, X., Wang, J., Liu, C., Grizzle, W.E., Yu, S., Zhang, S., Barnes, S., Koopman, W.J., Mountz, J.D., Kimberly, R.P., and Zhang, H-G.
    Cleavage of p53-vimentin complex enhances tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis of rheumatoid arthritis synovial fibroblasts.
    American Journal of Pathology, 167: 705-719, 2005.

  9. Kaput, J., Ordovas, J.M., Ferguson, L., van Ommen, B, Rodriguez, R.L., Allen, L., Ames, B.N., Dawson, K., German, B., Krauss, R., Malyj, W., Archer, M.C., Barnes, S., Bartholomew, A., Birk, R., van Bladeren, P., Bradford, K.J., Brown, K.H., Caetano, R., Castle, D., Chadwick, R., Clarke, S., Clément, K., Corella. D., da Cruz, I.M., Daniel, H., Duster, T., Sven O.E. Ebbesson, S.O, Elliott, R, Fairweather-Tait, S., Felton, J., Fenech, M., Finley, J.W., Fogg-Johnson, N., Gill-Garrison, R., Gibney, M.J., Gillies, P.J., Gustafsson, J-A.,Hartman, J.L. IV, He, L., Hwang, J-K., Jang, Y., Joost, H., Junien, C., Kanter, M., Kibbe, W.A., Koletzko, B., Korf, B.R., Kornman, K., Krempin, D.W., Langin, D., Lauren, D.R., Lee, J.H., Leveille, G.A., Lin, S-J., Mathers, J., Mayne, M., McNabb, W., Milner, J.A., Morgan, P., Muller, M., Nikolsky, Y., van der Ouderaa, F., Park, T., Pensel, N., Perez-Jimenez, F., Jean-Philippe, Kaisa Poutanen, Roberts, M., Saris, W., Schuster, G., Shelling, A.N., Simopoulos, A.P., Southon, S., Tai, E.S., Towne, B, Trayhurn, P., Uauy, R., Willard J. Visek, W.J., Warden, C., Weiss, R., Wiencke, J., Winkler, J., Wolff, G.L., Zhao-Wilson, X., Zucker, J-D.
    The Case for Strategic International Alliances to Harness Nutritional Genomics for Public and Personal Health.
    British Journal of Nutrition, 94: 623-632, 2005.

  10. Meezan, E., Meezan, E.M., Jones, K., Moore II, D.R., Barnes, S., and Prasain, J.K.
    Contrasting effects of the isoflavones glucosides puerarin and daidzein on glucose metabolism in mice.
    Journal of Agricultural and Food Chemistry. 53(22):8760-7, 2005.

  11. Zakharkin S.O., Kim, K., Mehta, T., Chen, L., Barnes, S., Scheirer, K.E., Parrish, R.S., Allison, D.B., and Page, G.P.
    Sources of variation in Affymetrix microarray experiments.
    BMC Bioinformatics, 6: 214, 2005.

  12. Huang, C.M., Wang, C.C., Kawai, M., Barnes, S., and Elmets, C.A.
    Surfactant sodium lauryl sulfate enhances skin vaccination: Molecular characterization via a novel technique using ultrafiltration capillaries and mass spectrometric proteomics.
    Mass Spectrometry Reviews, 24: 647-660, 2005.

  13. Huang, C.M., Elmets, C.A., van Kampen, K.R., DeSilva, T.S., Barnes, S., Kim, H., and Tang, D.C.
    Prospective highlights of functional skin proteomics.
    Mass Spectrometry Reviews, 24: 647-660, 2005.
        This is an invited review on the application of proteomics and mass spectrometry techniques to explore the proteome of the skin in disease.

  14. Shonsey EM, Sfakianos M, Johnson M, He D, Falany CN, Falany J, Merkler DJ, Barnes S.
    Bile acid coenzyme A: amino acid N-acyltransferase in the amino acid conjugation of bile acids.
    Methods Enzymol. 400:374-94, 2005.

  15. Shonsey EM, Wheeler J, Johnson M, He D, Falany CN, Falany J, Barnes S.
    Synthesis of bile acid coenzyme a thioesters in the amino acid conjugation of bile acids.
    Methods Enzymol. 400:360-73, 2005.

  16. D'Alessandro TL, Boersma-Maland BJ, Peterson TG, Sfakianos J, Prasain JK, Patel RP, Darley-Usmar VM, Botting NP, Barnes S.
    Metabolism of phytoestrogen conjugates.
    Methods Enzymol. 2005;400:316-42, 2005.


  1. Barnes S.
    Soy isoflavones--phytoestrogens and what else?
    J Nutr. 134(5):1225S-1228S, 2004.

  2. Schuler, A.M., Barnes, S., Gower, B.A., and Wood, P.A.
    Dietary Phytoestrogens Increase Metabolic Resistance (Cold Tolerance) in Long-Chain Acyl-CoA Dehydrogenase-Deficient Mice
    Journal of Nutrition, 134:1028-1031, May 2004.

  3. Stephen Barnes and Helen Kim
    Nutriproteomics: Identifying the Molecular Targets of Nutritive and Non-nutritive Components of the Diet.
    Journal of Biochemistry and Molecular Biology, 37: 59-74, 2004.
        This review concerns how to apply proteomics and mass spectrometry to the discovery of the biologically active components of the diet.

  4. Helen Kim, PhD, Grier P. Page, PhD, and Stephen Barnes, PhD
    Proteomics and Mass Spectrometry in Nutrition Research.
    Nutrition, 20:155-165, 2004.
        This paper reviews the technologies of 2D-gel proteomics and mass spectrometry (MALDI and nanoLC-tandem mass spectrometry) as they can be applied to modern nutrition research with particular reference to experimental design. It is part of a special issue of Nutrition on nutrigenomics.

  5. Prasain, J.K., Jones, K., Brissie, N., Moore, D.R. II, Wyss, J.M., Barnes, S.
    Identification of puerarin and its metabolites in rats by liquid chromatography-tandem mass spectrometry.
    Journal of Agricultural Food Chemistry, 52: 3708-3712, 2004.
        This piece of work established a quantitative L C-MRM-MS assay for the measurement of puerarin. It was applied to studies in rats of the uptake and metabolism of this Kudzu isoflavone. It was shown that puerarin was absorbed intact and rapidly excreted in urine. It underwent minor metabolism to mono- and dihydroxylated forms as well as to daidzein and equol after 2-3 days.

  6. Foxwell, A.I., Wilson, L., Kirk, M., Coward, L., Darley-Usmar, V.M. and Barnes, S.
    Modification of cytochrome c by 4-hydroxy-2-nonenal: evidence for histidine, lysine and arginine-aldehyde adducts.
    Journal of American Society of Mass Spectrometry 15: 1136-1147, 2004.
        The reaction of cytochrome c with the reactive aldehyde 4HNE formed from oxidation of unsaturated fatty acid was studied using MALDI-TOF-MS and nanoLC-tandem mass spectrometry. Formation of up to four 4HNE adducts was discernable, three of which were fully characterized. This is the first demonstration of an arginine adduct.

  7. Prasain, J., Wang, C-C., and Barnes, S.
    Mass spectrometric methods for the determination of flavonoids in biological samples.
    Free Radical Biology and Medicine, 37: 1324-1350, 2004.
        This invited paper is an extensive review of the use of mass spectrometry for the analysis of flavonoids. It includes GC-MS, LC-MS and capillary electrophoresis-MS and MALDI-TOF, ESI, ion trap and FT-ICR techniques.

  8. Deshane, J., Chaves, L., Sarikonda, K.V, Wilson, L., Isbell, S., Kirk, M.C., Grubbs, C, Barnes, S., Meleth, S., and Kim, H.
    Proteomics Analysis of Rat Brain Protein Modulations by Grape Seed Extract
    Journal of Agricultural Food Chemistry, 52: 7872-7883, 2004.

  9. Kim, H., Hall, P., Smith, M., Kirk, M., Prasain, J.K., Barnes, S., Grubbs, C.
    Chemoprevention by grapeseed extract and genistein in carcinogen-induced mammary cancer in rats is diet-dependent.
    Journal of Nutrition, 134: 3445S-3552S, 2004.

  10. Allison DB, Barnes S, Garvey WT.
    Individual differences in the response to ingested nutrients.
    Nutrition. 20(1):1, 2004.


  1. Page G.P., Edwards J.W., Barnes S., and Allison, D.B.
    A Design & Statistical Perspective on Microarray Gene.
    Expression Studies in Nutrition - the need for playful creativity and scientific hard-mindedness.

    Nutrition, 19: 997-1000, 2003.

  2. Boersma, B.J., Patel, R.P., Benton, M.R., Kirk, M., Wilson, L.S., Botting, N.P., Barnes, S., and Darley-Usmar, V.M.
    Neutrophil myeloperoxidase chlorinates soy isoflavones and enhances their antioxidant properties.
    Free Radical Biology and Medicine, 35:1417-1430, 2003.

  3. D'Alessandro, T., Prasain, J., Botting, N.P, Moore, R., Darley-Usmar, V.M., Patel, R.P., and Barnes, S.
    Polyphenols, Inflammatory Response, and Cancer Prevention: Chlorination of Isoflavones by Human Neutrophils.
    J Nutr,133:3773S-3777S, 2003.

  4. He, D., Barnes, S, Falany, C.N.
    Rat Liver Bile Acid CoA: Amino Acid N-acyltransferase: Expression, Characterization and Peroxisomal Localization
    Journal of Lipid Research, on line September 1, 2003.

  5. Carballal, S., Radi, R., Kirk, M.C., Barnes, S., Freeman, B.A.and Alvarez, B.
    Sulfenic acid formation in human serum albumin by hydrogen peroxide and peroxynitrite.
    Biochemistry, 42: 9906-9914, 2003.

  6. Prasain, J.K., Boersma, B.J., Kirk, M., Wilson, L., Patel, R., Darley-Usmar, V.M., Botting, N. and Barnes, S.
    Mass spectrometric methods for the analysis of chlorinated and nitrated isoflavonoids: a novel class of biological metabolites..
    Journal of Mass Spectrometry, 38: 764-771, 2003.

  7. Prasain, J.K., Jones, K., Kirk, M., Wilson, L., Smith-Johnson, M., Weaver, C.M., Barnes, S.
    Profiling and quantification of isoflavonoids in kudzu dietary supplements by high-performance liquid chromatography and electrospray ionization tandem mass spectrometry.
    Journal of Agricultural Food Chemistry, 51:4213-4218, 2003.

  8. Aslan, M, Barnes, S., Kirk, M, Rosenfeld, S, Townes, T, Ryan, T and BA Freeman.
    Nitric oxide-dependent generation of reactive species in sickle cell disease: renal and hepatocellular actin tyrosine nitration.
    Journal of Biological Chemistry, 278: 4194-4204, 2003.

  9. Lanman, J., Lam, T., Barnes, S., Sakalian, M., Emmett, M.R., Marshall, A.G., and Prevelige, P.E., Jr.
    Identification of novel interactions in HIV-1 capsid protein assembly by high resolution mass spectrometry.
    Journal of Molecular Biology, 325:759-772, 2003.

  10. Belenky M, Prasain J, Kim H, Barnes S.
    DING, a genistein target in human breast cancer: a protein without a gene.
    J Nutr. 133(7 Suppl):2497S-2501S, 2003.

  11. Barnes S.
    Phyto-oestrogens and osteoporosis: what is a safe dose?
    Br J Nutr. 89 Suppl 1:S101-8, 2003.


  1. Sfakianos, M., Wilson, L., Sakalian, M., Falany, C.N., and Barnes, S.
    Conserved Residues in the Putative Catalytic Triad of Human Bile Acid Coenzyme A: Amino Acid N-acyltransferase.
    Journal of Biological Chemistry, 277: 47270-47275, 2002.

  2. Falany, C.N., Xie, X, Wheeler, J.B., Wang, J., Smith, M., He, D., and Barnes, S.
    Molecular cloning and expression of rat liver bile acid CoA ligase.
    Journal of Lipid Research, 43: 2062-2071, 2002.

  3. Kim, H., Chaves, L., Mills, R., Oh, J-Y., DeSilva T., Coward, L., Kirk, M. and Barnes, S.
    Effects of soy on proteins implicated in dementias and other neurodegenerative conditions: the use of proteomics technology to assess protein changes.
    In: Soy and Health, 2002: Clinical Evidence and Dietetic Applications, Ed. Descheemaeker, K., Debruyne, I., Garant Publishers, Antwerp-Apeldoorn, pp 155-166, 2002.

  4. Barnes, S.
    New technologies in soy research. What can they bring?
    In: Soy and Health 2002: Clinical Evidence and Dietetic Applications, Ed. Descheemaeker, K., Debruyne, I., Garant Publishers, Antwerp-Apeldoorn, pp 39-50, 2002.

  5. Prasain, J., Wang, C-C., and Barnes, S.
    Mass spectrometry in the analysis of phytoestrogens in biological samples.
    In: Phytoestrogens and Health, Gilani, G.S. and Anderson, J.J., editors, AOCS Press, Champaign, IL, pp 147-177, 2002.

  6. Wang, C-C., Prasain, J., and Barnes, S.
    Review of the methods used in the determination of phytoestrogens.
    Journal of Chromatography, 777: 3-28, 2002.

  7. Barnes, S. and Messina, M.
    Introduction and Satellite Session at the Fourth International Symposium on the Role of Soy in Preventing and Treating Chronic Disease.
    Journal of Nutrition, 132: 545-546, 2002.

  8. Persky, V.W., Turyk, M.E., Wang, L., Freels, S., Chatterton, R., Jr, Barnes, S., Erdman, J., Jr, Sepkovic, D.W., Bradlow, H.L. and Potter.S.
    Effect of soy protein on endogenous hormones in postmenopausal women.
    American Journal of Clinical Nutrition. 75: 145-153, 2002.

  9. Brookes, P.S., Pinner, A., Ramachandran, A., Coward, L., Barnes, S, Kim, H., Darley-Usmar, V.M..
    High throughput two-dimensional blue-native electrophoresis: A tool for functional proteomics of mitochondria and signaling complexes.
    Proteomics 2, 969-977, 2002.

  10. Barnes S, Wang CC, Kirk M, Smith-Johnson M, Coward L, Barnes NC, Vance G, Boersma B.
    HPLC-mass spectrometry of isoflavonoids in soy and the American groundnut, Apios americana.
    Adv Exp Med Biol. 505:77-88, 2002.

  11. Lim DG, Bloodsworth A, JP Eiserich, CR White, PH Chumley, S Sweeney, M Kirk, S Barnes, VB O'Donnell, and BA Freeman.
    Nitrolinoleate – A lipid signaling molecule generated from nitric oxide and reactive oxygen species: Synthesis, characterization and biological activity.
    Proc Nat Acad Sci, 99(25):15941-15946, 2002 [doi: 10.1073/pnas.232409599]


  1. Kim H, Xu J, Su Y, Xia H, Li L, Peterson G, Murphy-Ullrich J, Barnes S
    Actions of the soy phytoestrogen genistein in models of human chronic disease: potential involvement of transforming growth factor beta.
    Biochem Soc Trans. 29(Pt 2):216-22, 2001.

  2. Barnes S.
    Oestrogens and their promiscuous receptors: confronting reality.
    Biochem Soc Trans. 29(Pt 2):231-6, 20001. (Review)

  3. Barnes S.
    Role of phytochemicals in prevention and treatment of prostate cancer.
    Epidemiol Rev. 23(1):102-5, 2001. (Review)

  4. Patel, R.P., Boersma, B., Crawford, J.H., Hogg, N., Kirk, M., Kalyanaraman, B., Parks, D., Barnes, S. and Darley-Usmar, V.
    Antioxidant mechanisms of isoflavones in lipid systems: Paradoxical effects of peroxyl radical scavenging.
    Free Radical Biology and Medicine, 31:1570-1581, 2001.

  5. Whitaker, B.D., Schmidt, W.F., Kirk, M.C. and Barnes, S.
    Novel Fatty-Acid Esters of p-Coumaryl Alcohol in Epicuticular Wax of Apple Fruit.
    Journal of Agricultural Food Chemistry, 49; 3787-3792, 2001.

  6. Boersma, B., S. Barnes, M. Kirk, C-C. Wang, M. Smith, H. Kim, J. Xu, R. Patel and V.M. Darley-Usmar.
    Soy isoflavonoids and cancer - metabolism at the target site.
    Mutation Research, 480: 121-127, 2001.

  7. Zhang, C., Eiserich, J., Reiter, C, Parks, D.A., Darley-Usmar, V., Beckman, J.S., Barnes, S., Kirk, M., and White, C.R.
    L-Arginine chlorination products inhibit endothelial nitric oxide production.
    Journal of Biological Chemistry, 276: 27159-27165, 2001.

  8. Binsack, R., Boersma, B.J., Patel,, R.P., Kirk, M.C., White, C.R., Darley-Usmar, V.M., Barnes, S., Zhou, F., and Parks, D.A..
    Enhanced antioxidant activity following chlorination of quercetin by hypochlorous acid.
    Alcoholism: Clinical and Experimental Research, 25: 434-443, 2001.

  9. Tuma, R., Coward, L. Kirk, M.C., Barnes, S., and Prevelige, P.E., Jr.
    Hydrogen-deuterium Exchange as a Probe of Folding and Assembly in Viral Capsids
    Journal of Molecular Biology, 306: 389-396, 2001.

  10. Urban, D., Irwin, W., Kirk, M., Markiewicz, M.A., Myers, R., Smith, M., Weiss, H., Grizzle, W.E. and Barnes, S.
    The effect of isolated soy protein on plasma biomarkers in elderly men with elevated serum prostate specific antigen.
    Journal of Urology 165:294-300, 2001.

  11. Boersma BJ, Patel RP, Botting N, White CR, Parks D, Barnes S, Darley-Usmar VM.
    Formation of novel bioactive metabolites from the reactions of pro-inflammatory oxidants with polyphenolics.
    Biofactors.15(2-4):79-81, 2001. (Review)


  1. Novak, J., Tomana, M., Kilian, M., Coward, L., Kulhavy, R., Barnes, S. and Mestecky, J.
    Heterogeneity of O-glycosylation in the hinge region of human IgA1.
    Molecular Immunology, 37: 1047-1056, 2000.

  2. Krull, R.E., Chen, P., Novak, J., Kirk, M., Barnes, S., Baker, J., Krishna, N.R. and Caufield, P.W.
    Biochemical structural analysis of the lantibiotic mutacin II.
    Journal of Biological Chemistry 275:15845-15850, 2000.

  3. Rubbo, H., Radi, R., Anselmi, D., Kirk, M., Barnes, S., Butler, J., Eiserich, J.P., and Freeman, B.A.
    Nitric oxide reaction with lipid peroxyl radicals spares -tocopherol during lipid peroxidation. Greater oxidant activity from the pair nitric oxide/ -tocopherol than -tocopherol/ascorbate.
    Journal of Biological Chemistry 275: 10812-10818, 2000.

  4. King, L. III, S. Barnes, U. Glufke, M.E. Henz, M. Kirk, K.A. Merkler, J.C. Vederas, B.J. Wilcoz, and D.J. Merkler.
    The enzymatic formation of novel bile acid primary amides. A possible class of new mammalian hormones.
    Archives of Biochemistry and Biophysics, 374: 107-117, 2000.

  5. Barnes S, Boersma B, Patel R, Kirk M, Darley-Usmar VM, Kim H, Xu J.
    Isoflavonoids and chronic disease: mechanisms of action.
    Biofactors. 12(1-4):209-15, 2000. (Review)

  6. Barnes S, Kim H, Darley-Usmar V, Patel R, Xu J, Boersma B, Luo M.
    Beyond ERalpha and ERbeta: estrogen receptor binding is only part of the isoflavone story.
    J Nutr. 130(3):656S-7S, 2000. (Review)


  1. Boersma, B.J., Patel, R.P., Kirk, M., Darley-Usmar, V.M. and S.Barnes.
    Chlorination and Nitration of Soy Isoflavones.
    Archives of Biochemistry and Biophysics, 368: 265-275,1999.

  2. O'Donnell VB, Eiserich JP, Bloodsworth A, Chumley PH, Kirk M, Barnes S, Darley-Usmar VM, Freeman BA.
    Nitration of unsaturated fatty acids by nitric oxide-derived reactive species.
    Methods Enzymol. 301:454-70, 1999. (Review)

  3. O'Donnell VB, Eiserich JP, Chumley PH, Jablonsky MJ, Krishna NR, Kirk M, Barnes S, Darley-Usmar VM, Freeman BA.
    Nitration of unsaturated fatty acids by nitric oxide-derived reactive nitrogen species peroxynitrite, nitrous acid, nitrogen dioxide, and nitronium ion.
    Chem Res Toxicol. 12(1):83-92, 1999.


  1. Barnes S.
    Phytoestrogens and breast cancer.
    Baillieres Clin Endocrinol Metab. 12(4):559-79, 1998. (Review)

  2. Peterson TG, Ji GP, Kirk M, Coward L, Falany CN, Barnes S.
    Metabolism of the isoflavones genistein and biochanin A in human breast cancer cell lines.
    Am J Clin Nutr. 68(6 Suppl):1505S-1511S, 1998.

  3. Coward L, Smith M, Kirk M, Barnes S.
    Chemical modification of isoflavones in soyfoods during cooking and processing.
    Am J Clin Nutr. 68(6 Suppl):1486S-1491S, 1998.

  4. Kim H, Peterson TG, Barnes S.
    Mechanisms of action of the soy isoflavone genistein: emerging role for its effects via transforming growth factor beta signaling pathways.
    Am J Clin Nutr. 68(6 Suppl):1418S-1425S, 1998. (Review)

  5. Williams JP, Jordan SE, Barnes S, Blair HC.
    Tyrosine kinase inhibitor effects on avian osteoclastic acid transport.
    Am J Clin Nutr. 68(6 Suppl):1369S-1374S, 1998.

  6. Skinner KA, White CR, Patel R, Tan S, Barnes S, Kirk M, Darley-Usmar V, Parks DA.
    Nitrosation of uric acid by peroxynitrite. Formation of a vasoactive nitric oxide donor.
    J Biol Chem. 18;273(38):24491-7, 1998.

  7. Chen P, Novak J, Kirk M, Barnes S, Qi F, Caufield PW.
    Structure-activity study of the lantibiotic mutacin II from Streptococcus mutans T8 by a gene replacement strategy.
    Appl Environ Microbiol. 64(7):2335-40, 1998.

  8. Barnes S.
    Evolution of the health benefits of soy isoflavones.
    Proc Soc Exp Biol Med. 217(3):386-92, 1998.

  9. Lamartiniere CA, Murrill WB, Manzolillo PA, Zhang JX, Barnes S, Zhang X, Wei H, Brown NM.
    Genistein alters the ontogeny of mammary gland development and protects against chemically-induced mammary cancer in rats.
    Proc Soc Exp Biol Med. 217(3):358-64, 1998.

  10. Barnes S, Coward L, Kirk M, Sfakianos J.
    HPLC-mass spectrometry analysis of isoflavones.
    Proc Soc Exp Biol Med. 217(3):254-62, 1998.


  1. Barnes S.
    The chemopreventive properties of soy isoflavonoids in animal models of breast cancer.
    Breast Cancer Res Treat. 46(2-3):169-79, 1997. (Review)

  2. Crow JP, Ye YZ, Strong M, Kirk M, Barnes S, Beckman JS.
    Superoxide dismutase catalyzes nitration of tyrosines by peroxynitrite in the rod and head domains of neurofilament-L.
    J Neurochem. 69(5):1945-53, 1997.

  3. White CR, Moellering D, Patel RP, Kirk M, Barnes S, Darley-Usmar VM
    Formation of the NO donors glyceryl mononitrate and glyceryl mononitrite from the reaction of peroxynitrite with glycerol.
    Biochem J. 328 ( Pt 2):517-24, 1997.

  4. Wheeler JB, Shaw DR, Barnes S.
    Purification and characterization of a rat liver bile acid coenzyme A ligase from rat liver microsomes.
    Arch Biochem Biophys. 1348(1):15-24, 1997.

  5. Sfakianos J, Coward L, Kirk M, Barnes S.
    Intestinal uptake and biliary excretion of the isoflavone genistein in rats.
    J Nutr. 127(7):1260-8, 1997.

  6. Falany CN, Fortinberry H, Leiter EH, Barnes S.
    Cloning, expression, and chromosomal localization of mouse liver bile acid CoA:amino acid N-acyltransferase.
    J Lipid Res. 38(6):1139-48, 1997.


  1. Peterson G, Barnes S.
    Genistein inhibits both estrogen and growth factor-stimulated proliferation of human breast cancer cells.
    Cell Growth Differ. 7(10):1345-51, 1996.

  2. Peterson TG, Coward L, Kirk M, Falany CN, Barnes S.
    The role of metabolism in mammary epithelial cell growth inhibition by the isoflavones genistein and biochanin A.
    Carcinogenesis. 17(9):1861-9, 1996.

  3. Murrill WB, Brown NM, Zhang JX, Manzolillo PA, Barnes S, Lamartiniere CA.
    Prepubertal genistein exposure suppresses mammary cancer and enhances gland differentiation in rats.
    Carcinogenesis. 1996 Jul;17(7):1451-7, 1996.

  4. Blair HC, Jordan SE, Peterson TG, Barnes S.
    Variable effects of tyrosine kinase inhibitors on avian osteoclastic activity and reduction of bone loss in ovariectomized rats.
    J Cell Biochem. 15;61(4):629-37, 1996.

  5. Novák J, Kirk M, Caufield PW, Barnes S, Morrison K, Baker J.
    Detection of modified amino acids in lantibiotic peptide mutacin II by chemical derivation and electrospray ionization-mass spectroscopic analysis.
    Anal Biochem. 1;236(2):358-60, 1996.

  6. Coward L, Kirk M, Albin N, Barnes S.
    Analysis of plasma isoflavones by reversed-phase HPLC-multiple reaction ion monitoring-mass spectrometry.
    Clin Chim Acta. 247(1-2):121-42, 1996.

  7. Greis KD, Hayes BK, Comer FI, Kirk M, Barnes S, Lowary TL, Hart GW.
    Selective detection and site-analysis of O-GlcNAc-modified glycopeptides by beta-elimination and tandem electrospray mass spectrometry.
    Anal Biochem. 234(1):38-49, 1996.

  8. Barnes S, Sfakianos J, Coward L, Kirk M.
    Soy isoflavonoids and cancer prevention. Underlying biochemical and pharmacological issues.
    Adv Exp Med Biol. 401:87-100, 1996.


  1. Rubbo H, Parthasarathy S, Barnes S, Kirk M, Kalyanaraman B, Freeman BA.
    Nitric oxide inhibition of lipoxygenase-dependent liposome and low-density lipoprotein oxidation: termination of radical chain propagation reactions and formation of nitrogen-containing oxidized lipid derivatives.
    Arch Biochem Biophys. 324(1):15-25, 1995.

  2. Lamartiniere CA, Moore JB, Brown NM, Thompson R, Hardin MJ, Barnes S.
    Genistein suppresses mammary cancer in rats.
    Carcinogenesis. 16(11):2833-40, 1995.

  3. Barnes S.
    Effect of genistein on in vitro and in vivo models of cancer.
    J Nutr. 125(3 Suppl):777S-783S, 1995. (Review)

  4. Barnes S, Peterson TG, Coward L.
    Rationale for the use of genistein-containing soy matrices in chemoprevention trials for breast and prostate cancer.
    J Cell Biochem Suppl. 22:181-7, 1995.

  5. Wei H, Bowen R, Cai Q, Barnes S, Wang Y.
    Antioxidant and antipromotional effects of the soybean isoflavone genistein.
    Proc Soc Exp Biol Med. 208(1):124-30, 1995.

  6. Lamartiniere CA, Moore J, Holland M, Barnes S.
    Neonatal genistein chemoprevents mammary cancer.
    Proc Soc Exp Biol Med. 208(1):120-3, 1995.

  7. Barnes S, Peterson TG.
    Biochemical targets of the isoflavone genistein in tumor cell lines.
    Proc Soc Exp Biol Med. 208(1):103-8, 1995. (Review)


  1. Falany CN, Johnson MR, Barnes S, Diasio RB.
    Glycine and taurine conjugation of bile acids by a single enzyme. Molecular cloning and expression of human liver bile acid CoA:amino acid N-acyltransferase.
    J Biol Chem. 269(30):19375-9, 1994.

  2. Barnes S, Peterson G, Grubbs C, Setchell K.
    Potential role of dietary isoflavones in the prevention of cancer.
    Adv Exp Med Biol. 354:135-47, 1994.


  1. Lin A, Wallace RW, Barnes S.
    Purification and properties of a human platelet inositol 1,4,5-trisphosphate 3-kinase.
    Arch Biochem Biophys. 303(2):412-20, 1993.

  2. Peterson G, Barnes S.
    Genistein and biochanin A inhibit the growth of human prostate cancer cells but not epidermal growth factor receptor tyrosine autophosphorylation.
    Prostate. 22(4):335-45, 1993.

  3. Kwakye JB, Barnes S, Diasio RB.
    Identification of bile acid coenzyme A synthetase in rat kidney.
    J Lipid Res. 1993 Jan;34(1):95-9.

  4. Wei H, Wei L, Frenkel K, Bowen R, Barnes S.
    Inhibition of tumor promoter-induced hydrogen peroxide formation in vitro and in vivo by genistein.
    Nutr Cancer. 20(1):1-12, 1993.


  1. Zhang R, Barnes S, Diasio RB.
    Differential intestinal deconjugation of taurine and glycine bile acid N-acyl amidates in rats.
    Am J Physiol. 262(2 Pt 1):G351-8, 1992.

  2. Zhang RW, Soong SJ, Liu TP, Barnes S, Diasio SB.
    Pharmacokinetics and tissue distribution of 2-fluoro-beta-alanine in rats. Potential relevance to toxicity pattern of 5-fluorouracil.
    Drug Metab Dispos. 20(1):113-9, 1992.

  3. Falany CN, Wheeler J, Coward L, Keehan D, Falany JL, Barnes S.
    Bioactivation of 7-hydroxymethyl-12-methylbenz[a]anthracene by rat liver bile acid sulfotransferase I.
    J Biochem Toxicol. 7(4):241-8, 1992.


  1. Kwakye JB, Johnson MR, Barnes S, Grizzle WE, Diasio RB.
    Identification of bile acid-CoA: amino acid N-acyltransferase in rat kidney.
    Biochem J. 280 ( Pt 3):821-4, 1991.

  2. Peterson G, Barnes S.
    Genistein inhibition of the growth of human breast cancer cells: independence from estrogen receptors and the multi-drug resistance gene.
    Biochem Biophys Res Commun. 179(1):661-7, 1991.

  3. Johnson MR, Barnes S, Kwakye JB, Diasio RB.
    Purification and characterization of bile acid-CoA:amino acid N-acyltransferase from human liver.
    J Biol Chem. 266(16):10227-33, 1991.

  4. Sweeny DJ, Barnes S, Diasio RB.
    Bile acid conjugation pattern in the isolated perfused rat liver during infusion of an amino acid formulation.
    JPEN J Parenter Enteral Nutr. 15(3):303-6, 1991.

  5. Zhang RW, Barnes S, Diasio RB.
    Disposition and metabolism of 2-fluoro-beta-alanine conjugates of bile acids following secretion into bile.
    Biochim Biophys Acta. 1991 Apr 15;1096(3):179-86.

  6. Kwakye JB, Johnson MR, Barnes S, Diasio RB.
    A comparative study of bile acid CoA:amino acid:N-acyltransferase (BAT) from four mammalian species.
    Comp Biochem Physiol B. 100(1):131-6, 1991.


  1. Johnson MR, Barnes S, Sweeny DJ, Diasio RB.
    2-Fluoro-beta-alanine, a previously unrecognized substrate for bile acid coenzyme A:amino acid:N-acyltransferase from human liver.
    Biochem Pharmacol. 40(6):1241-6, 1990.

  2. Lin AN, Barnes S, Wallace RW.
    Phosphorylation by protein kinase C inactivates an inositol 1,4,5-trisphosphate 3-kinase purified from human platelets.
    Biochem Biophys Res Commun. 170(3):1371-6, 1990.

  3. Sweeny DJ, Daher G, Barnes S, Diasio RB.
    Biological properties of the 2-fluoro-beta-alanine conjugates of cholic acid and chenodeoxycholic acid in the isolated perfused rat liver.
    Biochim Biophys Acta. 1054(1):21-5, 1990.

  4. Barnes S, Grubbs C, Setchell KD, Carlson J.
    Soybeans inhibit mammary tumors in models of breast cancer.
    Prog Clin Biol Res. 239-53, 1990.


  1. Johnson MR, Barnes S, Diasio RB.
    Radioassay of bile acid coenzyme A:glycine/taurine: N-acyltransferase using an n-butanol solvent extraction procedure.
    Anal Biochem. 182(2):360-5, 1989.

  2. Barnes S, Buchina ES, King RJ, McBurnett T, Taylor KB.
    Bile acid sulfotransferase I from rat liver sulfates bile acids and 3-hydroxy steroids: purification, N-terminal amino acid sequence, and kinetic properties.
    J Lipid Res. 1989 Apr;30(4):529-40.


  1. Sweeny DJ, Barnes S, Diasio RB.
    Formation of conjugates of 2-fluoro-beta-alanine and bile acids during the metabolism of 5-fluorouracil and 5-fluoro-2-deoxyuridine in the isolated perfused rat liver.
    Cancer Res. 48(8):2010-4, 1988.


  1. Mukidjam E, Elgavish GA, Barnes S.
    Structure of the dysprosium-glycocholate complex in submicellar aqueous solution: paramagnetic mapping by proton nuclear magnetic resonance spectroscopy. An approximation for the intrinsic "bound" relaxation rates in the case of nondilute paramagnetic systems.
    Biochemistry. 26(21):6785-92, 1987.

  2. Hayes SM, Liu RH, Tsang WS, Legendre MG, Berni RJ, Pillion DJ, Barnes S, Ho MH.
    Enantiomeric composition analysis of amphetamine and methamphetamine by chiral phase high-performance liquid chromatography-mass spectrometry.
    J Chromatogr. 398:239-46, 1987.