• CFTR Cell Model and Assay Core
    CFTR Cell Model and Assay Core

    Director: Kevin L. Kirk, Ph.D.
    Email: GFJCFRC@uab.edu
    Phone: 934-3122

    Website:  http://www.uab.edu/cfcenter

    The Core provides epithelial cell lines expressing CFTR; assists with patch clamp and single channel analysis relevant to ion channel gating; histochemical and Ussing Chamber analysis of CFTR .  Core users are typically associated with the Cystic Fibrosis Research Center, but there is no limitation on who may access the Core. 

    If you have any questions please contact us at:  GFJCFRC@uab.edu

    Core capabilities include:

    • Macroscopic assays of CFTR function
    • SPQ
    • Ussing chamber
    • Histochemical assays
    • RT-PCR
    • Mouse primary airway epithelial cell culture
    • Human airway epithelial cell line culture
    • Lentiviral cell line derivation
    • Zinc finger endonuclease based cell technologies
    • Whole cell membrane patch analysis
    • Single channel patch analysis
    • Macropatch analysis