• UAB Zebrafish Facility Resource
    UAB Zebrafish Facility Resource

    Director: Dr. Susan C. Farmer and Dr. Stephen Watts
    Email: sfarmer@uab.edu or sawatts@uab.edu
    Phone: 934-5563 (Dr. Farmer) or 934-8313 (Dr.Watts)

    Contemporary biomedical research relies heavily on the use of experimental animal model systems that recapitulate mammalian biological processes. The zebrafish (Danio rerio) has become an important model organism for the study of vertebrate development and disease, organ function, behavior, toxicology, cancer, and drug discovery.  Zebrafish produce a large number of offspring at low cost, which is convenient for large genetic or molecular screens.  The zebrafish genome has been sequenced, their genetic manipulation is relatively easy, and protocols are established for transgene expression or morpholino knockdown in embryos.   The UAB Zebrafish Research Facility is the product of collaborative support from the UAB Research Community.  The 5000 net square feet main core resource is located in the Research Support Building (RSB) and includes recirculating aquaria systems with a central water conditioning/purification system supplying 27 racks (>2200 3 L tank capacity).  The in vitro manipulation laboratory includes four embryos injection stations, a pipette puller, a fluorescent scope and camera, a cell press, breeding equipment, and support supplies. There is an online system for reserving equipment in the in vitro manipulation laboratory.  The zebrafish quarantine facility is located in RSB but on a different floor to insure biosecurity. Animal husbandry (feeding, cleaning) is provided by the Animal Resources Program staff.  This resource is open to all UAB researchers using zebrafish as a research model. 

    Equipment
    Main facility: 25 rack recirculating water zebrafish husbandry system (2100 3 L tank capacity), 2 rack dark box recirculating water zebrafish husbandry system (144 3 L tank capacity), four embryo injection stations (micromanipulators, injectors, dissection scopes), one fluorescent microscope with camera, needle puller, cell press, 500 breeding tanks, zebrafihs enrichment equipment, live food production equipment (Artemia, rotifer)<br /> Quarantine facility:  Four stand alone zebrafish husbandry racks (288 3 L tank capacity), 100 breeding tanks, live food production equipment (Artemia)


    Center Affiliation
    Center for Clinical and Translational Science (CCTS)
    Comprehensive Cancer Center
    Gregory Fleming James Cystic Fibrosis Center
    Heflin Center for Genomic Science (HCGS)
    Nutrition Obesity Research Center

    Serves - Internal UAB Only