UAB Diabetes Research Center BioAnalytical Redox Biology (BARB) Core

Research Administration Centers and Cores UAB Diabetes Research Center BioAnalytical Redox Biology (BARB) Core

UAB Diabetes Research Center BioAnalytical Redox Biology (BARB) Core

Directory Category: Core

The Bio-Analytical Redox Biology (BARB) Core promotes redox biology in diabetes-related research, emphasizing that diabetes is both a metabolic and a vascular disease. Because it provides a combination of services not available through any other UAB cores or regional universities, the BARB Core serves as a hub to facilitate and promote the development of multi-disciplinary research projects among independently funded research programs in diabetes. 

The BARB Core promotes research in areas common to the metabolic and vascular aspects of diabetes and cardiovascular disease. The core provides state-of-the-art services in mitochondrial damage, function, proteomics, and oxidative stress assessment support for investigators carrying out diabetes mellitus (DM) and cardiometabolic disease (CMD) research at UAB. 

Furthermore, as the UAB DRC promotes interactions between outstanding groups of investigators at UAB working in metabolism and in vascular biology to better understand the pathophysiology of diabetes, the BARB Core facilitates the study of mitochondrial dysfunction and oxidative stress, which are key molecular processes contributing to both metabolic and vascular pathology. Thus, the core allows investigators to interact around these common research themes.

The BARB Core provides a series of molecular assays that will measure selected markers of oxidant stress, mitochondrial bioanalytical services, and quantification of atherosclerotic lesions. The selected methods are carefully geared to the needs of the DRC investigators, which is handled through a two-way process involving both the investigator and the core. 
The following sections briefly describe these methodologies.

Interaction with Other Cores
The BARB Core interacts with other DRC cores as well as non-DRC cores at UAB. The interactions with other DRC cores are critical to providing seamless services when assistance is required from multiple cores around a single project. Specifically, the BARB Core interacts with the Animal Physiology DRC Core for tissue collection; the Human Biology Core (e.g., plasma biomarker assays correlating with mtDNA damage and haplotype analyses), and Metrics (epidemiology of disease risk factors, mtDNA damage and haplotype assessment). Important interactions with cores outside the DRC include the UAB Comprehensive Cancer Center (CCC) Proteomics/Mass Spectrometry Facility (Dr. Stephen Barnes). The BARB Core provides a unique portfolio of services that does not duplicate any other UAB core facility.