Igor Dimitriev, Ph.D.
Assistant Professor
Division of Human Gene Therapy
Department of General Surgery/Oncology

570 Biomedical Research Building II
901 19th Street South
Birmingham, AL 35294-2172, USA

Email: dmitriev@uab.edu
Phone: (205) 975-8733
Fax: (205) 975-7949


Research Interests

My research interests include gene delivery to cancer cells and tumor tissue. As a means to deliver therapeutic/protecting genes we use recombinant adenovirus. Our major efforts are concentrated on alteration of the virus tropism by employment of molecular adaptors and direct genetic modifications of viral capsid. The methods we use in our studies include molecular biology and gene engineering.

Select List of Publications

  1. Dmitriev, I. P., Khromykh, A. A., Ignatyev, G. M., Gainullina, M. N., Ageenko, V. A., Dryga, S. A., Vorobyeva, M. S., and Sandakhchiev, L. S. Immunization with recombinant vaccinia viruses expressing structural and part of the nonstructural region of tick-borne encephalitis virus cDNA protect mice against lethal encephalitis, J Biotechnol. 44: 97-103, 1996.

  2. Chepurnov, A. A., Ternovoi, V. A., Dadaeva, A. A., Dmitriev, I. P., Sizikova, L. P., Volchkov, V. E., Kudoiarova, N. M., Rudzevich, T. N., and Netesov, S. V. Immunobiological properties of vp24 protein of Ebola virus expressed by recombinant vaccinia virus, Vopr Virusol [Russian]. 42: 115-20, 1997.

  3. Dmitriev, I., Krasnykh, V., Miller, C. R., Wang, M., Kashentseva, E., Mikheeva, G., Belousova, N., and Curiel, D. T. An adenovirus vector with genetically modified fibers demonstrates expanded tropism via utilization of a coxsackievirus and adenovirus receptor-independent cell entry mechanism, J Virol. 72: 9706-13, 1998.

  4. Krasnykh, V., Dmitriev, I., Mikheeva, G., Miller, C. R., Belousova, N., and Curiel, D. T. Characterization of an adenovirus vector containing a heterologous peptide epitope in the HI loop of the fiber knob, J Virol. 72: 1844-52, 1998.

  5. Douglas, J. T., Miller, C. R., Kim, M., Dmitriev, I., Mikheeva, G., Krasnykh, V., and Curiel, D. T. A system for the propagation of adenoviral vectors with genetically modified receptor specificities, Nat Biotechnol. 17: 470-5, 1999.

  6. Kasono, K., Blackwell, J. L., Douglas, J. T., Dmitriev, I., Strong, T. V., Reynolds, P., Kropf, D. A., Carroll, W. R., Peters, G. E., Bucy, R. P., Curiel, D. T., and Krasnykh, V. Selective gene delivery to head and neck cancer cells via an integrin targeted adenoviral vector, Clin Cancer Res. 5: 2571-9, 1999.

  7. Reynolds, P., Dmitriev, I., and Curiel, D. Insertion of an RGD motif into the HI loop of adenovirus fiber protein alters the distribution of transgene expression of the systemically administered vector, Gene Ther. 6: 1336-9, 1999.

  8. Blackwell, J. L., Li, H., Gomez-Navarro, J., Dmitriev, I., Krasnykh, V., Richter, C. A., Shaw, D. R., Alvarez, R. D., Curiel, D. T., and Strong, T. V. Using a tropism-modified adenoviral vector to circumvent inhibitory factors in ascites fluid, Hum Gene Ther. 11: 1657-69., 2000.

  9. Dmitriev, I., Kashentseva, E., Rogers, B. E., Krasnykh, V., and Curiel, D. T. Ectodomain of coxsackievirus and adenovirus receptor genetically fused to epidermal growth factor mediates adenovirus targeting to epidermal growth factor receptor-positive cells, J Virol. 74: 6875-84, 2000.

  10. Krasnykh, V., Dmitriev, I., Navarro, J. G., Belousova, N., Kashentseva, E., Xiang, J., Douglas, J. T., and Curiel, D. T. Advanced generation adenoviral vectors possess augmented gene transfer efficiency based upon coxsackie adenovirus receptor-independent cellular entry capacity, Cancer Res. 60: 6784-7, 2000.

  11. Hemminki, A., Dmitriev, I., Liu, B., Desmond, R. A., Alemany, R., and Curiel, D. T. Targeting oncolytic adenoviral agents to the epidermal growth factor pathway with a secretory fusion molecule, Cancer Res. 61: 6377-81, 2001.

  12. Wesseling, J. G., Bosma, P. J., Krasnykh, V., Kashentseva, E. A., Blackwell, J. L., Reynolds, P. N., Li, H., Parameshwar, M., Vickers, S. M., Jaffee, E. M., Huibregtse, K., Curiel, D. T., and Dmitriev, I. Improved gene transfer efficiency to primary and established human pancreatic carcinoma target cells via epidermal growth factor receptor and integrin-targeted adenoviral vectors, Gene Ther. 8: 969-76., 2001.

  13. Bauerschmitz, G. J., Lam, J. T., Kanerva, A., Suzuki, K., Nettelbeck, D. M., Dmitriev, I., Krasnykh, V., Mikheeva, G. V., Barnes, M. N., Alvarez, R. D., Dall, P., Alemany, R., Curiel, D. T., and Hemminki, A. Treatment of ovarian cancer with a tropism modified oncolytic adenovirus, Cancer Res. 62: 1266-70, 2002.

  14. Bilbao, G., Contreras, J. L., Dmitriev, I., Smyth, C. A., Jenkins, S., Eckhoff, D., Thomas, F., Thomas, J., and Curiel, D. T. Genetically modified adenovirus vector containing an RGD peptide in the HI loop of the fiber knob improves gene transfer to nonhuman primate isolated pancreatic islets, Am J Transplant. 2: 237-43., 2002.

  15. Dmitriev, I. P., Kashentseva, E. A., and Curiel, D. T. Engineering of adenovirus vectors containing heterologous peptide sequences in the C terminus of capsid protein IX, J Virol. 76: 6893-9., 2002.

  16. Haviv, Y. S., Blackwell, J. L., Kanerva, A., Nagi, P., Krasnykh, V., Dmitriev, I., Wang, M., Naito, S., Lei, X., Hemminki, A., Carey, D., and Curiel, D. T. Adenoviral gene therapy for renal cancer requires retargeting to alternative cellular receptors, Cancer Res. 62: 4273-81., 2002.

  17. Kashentseva, E. A., Seki, T., Curiel, D. T., and Dmitriev, I. P. Adenovirus targeting to c-erbB-2 oncoprotein by single-chain antibody fused to trimeric form of adenovirus receptor ectodomain, Cancer Res. 62: 609-16, 2002.

  18. Pereboev, A. V., Asiedu, C. K., Kawakami, Y., Dong, S. S., Blackwell, J. L., Kashentseva, E. A., Triozzi, P. L., Aldrich, W. A., Curiel, D. T., Thomas, J. M., and Dmitriev, I. P. Coxsackievirus-adenovirus receptor genetically fused to anti-human CD40 scFv enhances adenoviral transduction of dendritic cells, Gene Ther. 9: 1189-93., 2002.

  19. Seki, T., Dmitriev, I., Kashentseva, E., Takayama, K., Rots, M., Suzuki, K., and Curiel, D. T. Artificial extension of the adenovirus fiber shaft inhibits infectivity in coxsackievirus and adenovirus receptor-positive cell lines, J Virol. 76: 1100-8, 2002.

  20. Seki, T., Dmitriev, I., Suzuki, K., Kashentseva, E., Takayama, K., Rots, M., Uil, T., Wu, H., Wang, M., and Curiel, D. T. Fiber shaft extension in combination with HI loop ligands augments infectivity for CAR-negative tumor targets but does not enhance hepatotropism in vivo, Gene Ther. 9: 1101-8., 2002.

  21. Wu, H., Dmitriev, I., Kashentseva, E., Seki, T., Wang, M., and Curiel, D. T. Construction and characterization of adenovirus serotype 5 packaged by serotype 3 hexon, J Virol. 76: 12775-82., 2002.

  22. Zhang, H. G., Xie, J., Dmitriev, I., Kashentseva, E., Curiel, D. T., Hsu, H. C., and Mountz, J. D. Addition of six-His-tagged peptide to the C terminus of adeno- associated virus VP3 does not affect viral tropism or production, J Virol. 76: 12023-31., 2002.
  23. Barker, S. D., Dmitriev, I. P., Nettelbeck, D. M., Liu, B., Rivera, A. A., Alvarez, R. D., Curiel, D. T., and Hemminki, A. Combined transcriptional and transductional targeting improves the specificity and efficacy of adenoviral gene delivery to ovarian carcinoma, Gene Ther. 10: 1198-204, 2003.

  24. Smeets, R. L., van de Loo, F. A., Joosten, L. A., Arntz, O. J., Bennink, M. B., Loesberg, W. A., Dmitriev, I. P., Curiel, D. T., Martin, M. U., and van den Berg, W. B. Effectiveness of the soluble form of the interleukin-1 receptor accessory protein as an inhibitor of interleukin-1 in collagen-induced arthritis, Arthritis Rheum. 48: 2949-58, 2003.

  25. Uil, T. G., Seki, T., Dmitriev, I., Kashentseva, E., Douglas, J. T., Rots, M. G., Middeldorp, J. M., and Curiel, D. T. Generation of an adenoviral vector containing an addition of a heterologous ligand to the serotype 3 fiber knob, Cancer Gene Ther. 10: 121-4., 2003.

  26. Le, L. P., Everts, M., Dmitriev, I. P., Davydova, J. G., Yamamoto, M., and Curiel, D. T. Fluorescently labeled adenovirus with pIX-EGFP for vector detection, Mol Imaging. 3: 105-16, 2004.

  27. Liang, Q., Dmitriev, I., Kashentseva, E., Curiel, D. T., and Herschman, H. R. Noninvasive of adenovirus tumor retargeting in living subjects by a soluble adenovirus receptor-epidermal growth factor (sCAR-EGF) fusion protein, Mol Imaging Biol. 6: 385-94, 2004.

  28. Wu, H., Han, T., Lam, J. T., Leath, C. A., Dmitriev, I., Kashentseva, E., Barnes, M. N., Alvarez, R. D., and Curiel, D. T. Preclinical evaluation of a class of infectivity-enhanced adenoviral vectors in ovarian cancer gene therapy, Gene Ther. 11: 874-8, 2004.

  29. Hosokawa, M., Kadota, R., Shichijo, S., Itoh, K., Dmitriev, I., Krasnykh, V., Curiel, D. T., Takue, Y., Wakasugi, H., Takashima, S., and Heike, Y. Cell cycle arrest and apoptosis induced by SART-1 gene transduction, Anticancer Res. 25: 1983-90, 2005.

  30. Le, L. P., Le, H. N., Dmitriev, I. P., Davydova, J. G., Gavrikova, T., Yamamoto, S., Curiel, D. T., and Yamamoto, M. Dynamic monitoring of oncolytic adenovirus in vivo by genetic capsid labeling. J Natl Cancer Inst, 98: 203-214, 2006


Biosketch

Positions

2003 - Present Assistant Professor. University of Alabama at Birmingham, Departments of Medicine, Obstetrics and Gynecology, Pathology and Surgery, Division of Human Gene Therapy
2003 - Present Associate Scientist. University of Alabama at Birmingham Comprehensive Cancer Center
2004 - Present Associate Scientist. University of Alabama at Birmingham Gene Therapy Center

Previous Positions

2000 - 2003 Research Associate UAB; Departments of Medicine, Pathology and Surgery, Division of Human Gene Therapy
1996 - 2000 Postdoctoral Fellow UAB; Departments of Medicine, Pathology and Surgery, Gene Therapy Center
1986 - 1994 Junior Research Scientist, Laboratory of Recombinant Vaccines, Institute of Molecular Biology, SPA "VECTOR", Koltsovo, Novosibirsk region, Russia
1983 - 1985 Research Trainee, Institute of Molecular Biology, Scientific and Production Association (SPA) "VECTOR", Koltsovo, Novosibirsk region, Russia

Honors

1998John R. Durant Award for Excellence in Cancer Research


Awards

2003Junior Faculty Career Development Award


Academic Degrees

1994PhD in Molecular Biology. Institute of Molecular Biology, Scientific and Production Association (SPA) "VECTOR", Koltsovo, Novosibirsk region, Russia